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pIF1-S39E-GFP

Plasmid

#206937

Purpose

Plasmid expressing the mutant S39E of human IF1 fused in C-terminal with GFP

Depositor

José Cuezva

Article

Romero-Carraminana et al Commun Biol. 2023 Aug 12;6(1):836. doi:

Insert

S39E mutant of human IF1 (ATPase inhibitory factor 1)

Tags

GFP

Expression

Mammalian

Mutation

Changed serine 39 for a glutamic acid

Promoter

CMV

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pAM94

Plasmid

#226686

Purpose

GGG-TitinV13PV15P-23-K-9-C-25-intein-CBD

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM99

Plasmid

#226691

Purpose

TitinV15P-23-K-9-C-1

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM97

Plasmid

#226689

Purpose

GGG-Titinv15P-23-K-9-K-9-K-8-C-25

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM100

Plasmid

#226692

Purpose

TitinV15P-21-C-2-K-1

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM98

Plasmid

#226690

Purpose

TitinV15P-23-K-9-C-15

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM95

Plasmid

#226687

Purpose

GGG-TitinV15P-43-K-8-C-25-intein-CBD

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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PB-TO-hNIL

Plasmid

#172113

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

hNGN2 (NEUROG2 Human)
hISL1 (ISL1 Human)
hLHX3 (LHX3 Human)
puroR-T2A-mycNLS-mTagBFP2

Tags

T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and TRE3G

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-beta1-EGFP

Plasmid

#160978

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta1 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta1 (MOG Human)

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-BRAT1

Plasmid

#161920

Purpose

Expresses human BRAT1 with a N-terminal GFP-FLAG tag. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

BRCA1-associated ATM activator 1 (BRAT1 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-alpha2-EGFP

Plasmid

#160976

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform alpha2 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant alpha2 (MOG Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-alpha3-EGFP

Plasmid

#160977

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform alpha3 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant alpha3 (MOG Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-beta3-EGFP

Plasmid

#160980

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta3 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta3 (MOG Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5M-ADAM10

Plasmid

#31717

Depositor

Rik Derynck

Article

Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

Insert

ADAM10 (ADAM10 Human)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pTRIP CMV VHHG4

Plasmid

#182236

Purpose

Expresses GFP nanobody for stable expression in mammalian cells

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Insert

anti-GFP nanobody

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-Ago1

Plasmid

#21533

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago1 (AGO1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
JW617

Plasmid

#64809

Purpose

CUC3 expression in yeast

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC3 (CUC3 Mustard Weed)

Tags

GAL4 DNA binding domain and Myc

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pGWB21_TaNAM-A1

Plasmid

#124572

Purpose

Expresses full-length TaNAM-A1 with an N-terminal 10x Myc tag under the 35s promoter.

Depositor

Cristobal Uauy

Article

Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

Insert

TaNAM-A1

Tags

10xMyc

Expression

Plant

Promoter

35s

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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P3-dCas9-Tet1-GFP-Puro

Plasmid

#190728

Purpose

Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

Depositor

Maja Jagodic

Article

Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

Insert

dCas9-tet1

Use

CRISPR

Tags

3XFLAG and SV40 NLS

Expression

Mammalian

Mutation

Changed Pro434 codon from CCG to CCC and Gly468 f…

Promoter

pCAG

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCX-TRI-2A

Plasmid

#74674

Purpose

mammalian expression of TRI-2A (hHO1/F2A/hCD73/F2A/hCD39)

Depositor

Roberto Giovannoni

Article

Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

Inserts

HO1 (HMOX1 Human)
CD73 (NT5E Human)
CD39 (ENTPD1 Human)

Tags

F2A

Expression

Mammalian

Promoter

pCAGGS

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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