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CENPF C5.1 gRNA

Plasmid

#90616

Purpose

3rd generation lentiviral gRNA plasmid targeting human CENPF

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CENPF (Guide Designation C5.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-SARS-CoV-2 SL1 alone-Luciferase

Plasmid

#191482

Purpose

Luciferase reporter for SARS-CoV-2 5' UTR SL1 alone

Depositor

Hao Wu

Article

Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

Insert

SARS-CoV-2 5' UTR SL1 alone

Use

Lentiviral and Luciferase

Mutation

Contains only the stem-loop1 region of SARS-CoV-2…

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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ANAPC1 A2.3 gRNA

Plasmid

#90529

Purpose

3rd generation lentiviral gRNA plasmid targeting human ANAPC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ANAPC1 (Guide Designation A2.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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ANAPC1 A1.3 gRNA

Plasmid

#90528

Purpose

3rd generation lentiviral gRNA plasmid targeting human ANAPC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ANAPC1 (Guide Designation A1.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pD765 HMGCR 3'UTR

Plasmid

#105766

Purpose

Encodes the 3'UTR of relevant gene for validation of miRNAs targeting the 3'UTR in a dual luciferase assay

Depositor

Joseph Ziegelbauer

Article

Ramalingam et al Sci Rep. 2017 Jan 19;7:40813. doi: 10.10

Insert

HMGCR 3'UTR (HMGCR Human)

Use

Luciferase

Mutation

None

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pRB113.2

Plasmid

#181948

Purpose

aTc-inducible expression of NarL with C-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

NarL-mNG; mCherry; tetR (AX04_RS23485, narL Synthetic, narL - E. coli)

Tags

mNeonGreen

Expression

Bacterial

Promoter

NarL-mNG-PLtetO-1; mCherry-PdcuS; tetR-J23106

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

Plasmid

#115140

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA3 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

Plasmid

#115138

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA2 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pIS1 DICER1 long-mut-all

Plasmid

#21653

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated let-7 and miR103/107 microRNA binding sit…

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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UBE2C H5.2 gRNA

Plasmid

#90934

Purpose

3rd generation lentiviral gRNA plasmid targeting human UBE2C

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

UBE2C (Guide Designation H5.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pIS1 DICER1 long-mut-miR103/107

Plasmid

#21652

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant miR103/107 microRNA binding sites

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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NIPBL D8.4 gRNA

Plasmid

#90803

Purpose

3rd generation lentiviral gRNA plasmid targeting human NIPBL

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NIPBL (Guide Designation D8.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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NIPBL D7.4 gRNA

Plasmid

#90802

Purpose

3rd generation lentiviral gRNA plasmid targeting human NIPBL

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NIPBL (Guide Designation D7.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-p160(1-200)

Plasmid

#43

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pSUPER.retro BRIT1

Plasmid

#16206

Depositor

Shiaw-Yih Lin

Article

Rai et al Cancer Cell. 2006 Aug . 10(2):145-57.

Insert

shBRIT1 (MCPH1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA GAL4 CREB 1-341 M1

Plasmid

#22973

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

Insert

CREB (Creb1 Rat, GAL4-fusion)

Tags

GAL4 DNA-binding domain

Expression

Mammalian

Mutation

Changed Serine 133 to Alanine of CREB

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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ANLN A1.2 gRNA

Plasmid

#90530

Purpose

3rd generation lentiviral gRNA plasmid targeting human ANLN

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ANLN (Guide Designation A1.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_C2516b

Plasmid

#91085

Purpose

Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, (+ BaeI site for GT donor cloning), Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

At7SL

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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TUBG1 F7.4 gRNA

Plasmid

#90930

Purpose

3rd generation lentiviral gRNA plasmid targeting human TUBG1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TUBG1 (Guide Designation F7.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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NUDC D9.4 gRNA

Plasmid

#90806

Purpose

3rd generation lentiviral gRNA plasmid targeting human NUDC

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NUDC (Guide Designation D9.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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