165,947 results

1. pAAV-hSyn-GRAB_eCB2.0

   Plasmid

   #164604

   Purpose

   Express the endocannabinoid sensor GRAB_eCB2.0 in neurons

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid sensor GRAB_eCB2.0
   

   Use

   AAV

   Mutation

   S383T

   Promoter

   hSyn

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-p18-FLAG

   Plasmid

   #42331

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

   Insert

   p18 (LAMTOR1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q109

   Plasmid

   #111730

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q109 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-AviTag-DogCatcher-MBP

   Plasmid

   #171928

   Purpose

   Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   AviTag-DogCatcher-MBP
   

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   DogCatcher added to N-terminus of MBP via a GSGES…

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-4T-1-3xFLAG-HSP27

   Plasmid

   #129229

   Purpose

   Encodes FLAG tagged human HSP27 for purification by GST affinity

   Depositor

   Kevin Janes

   Article

   Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

   Insert

   HSP27 (HSPB1 Human)
   

   Tags

   FLAG and GST

   Expression

   Bacterial

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEvol-MjaYRS

   Plasmid

   #153557

   Purpose

   Amber suppression for incorporating 3-aminotyrosine to proteins in E. coli

   Depositor

   Huiwang Ai

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038

   Inserts

   MJaYRS (first copy)
   MJaYRS (second copy)
   amber suppression tRNA under proK promoter
   

   Expression

   Bacterial

   Promoter

   glnS and pBAD

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. 6xHis-SNAP-PI Probe

   Plasmid

   #211507

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteria

   Depositor

   Hannes Maib, David Murray

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   BcPI-PLC-ANH
   

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDY1513 AAVS1 sgRNA

   Plasmid

   #234832

   Purpose

   sgRNA for AAVS1 STITCHR insertion

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

   Insert

   AAVS1 sgRNA
   

   Use

   CRISPR

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF06R

   Plasmid

   #42940

   Depositor

   Eline Luning Prak

   Article

   Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

   Insert

   Human L1 element (L1RE1 Human)
   

   Tags

   EGFP (opposite strand)

   Expression

   Mammalian

   Promoter

   CMV MIE, L1 5'UTR

   Available Since

   April 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-C1-Nir1-LNS2

    Plasmid

    #220084

    Purpose

    high affinity PA biosensor with red fluorophore (also known as mCherry-PILS-Nir1)

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    PITPNM3 (PITPNM3 Human)
    

    Tags

    mCherry-GGSGGM

    Expression

    Mammalian

    Mutation

    amino acids 613-897

    Promoter

    CMV

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWALIUM10-V5-TurboID-GW

    Plasmid

    #196285

    Purpose

    Gateway destination vector to express N-terminal TurboID fusion under UAS promoter, for making transgenic flies

    Depositor

    Norbert Perrimon

    Type

    Empty backbone

    Use

    Gateway destination vector

    Expression

    Insect

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICE-HA-NLS-I-PpoI

    Plasmid

    #46963

    Purpose

    For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    NLS-I-PpoI
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV-TET

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. Str-KDEL_SBP-EGFP-LAMP2

    Plasmid

    #222321

    Purpose

    Synchronize the trafficking of LAMP2 from the ER.

    Depositor

    Franck Perez

    Article

    Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

    Insert

    Streptavidin-KDEL and LAMP2 fused to SBP-EGFP (LAMP2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Klotho (secreted)

    Plasmid

    #17713

    Depositor

    Hal Dietz

    Article

    Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

    Insert

    Klotho (KL Human)
    

    Tags

    His and V5

    Expression

    Mammalian

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDule2-Mb haloTyrRS C6

    Plasmid

    #160378

    Purpose

    C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

    Depositor

    Ryan Mehl

    Article

    Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

    Insert

    C6 HaloTyrosine tRNA synthatase
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Mammalian

    Mutation

    L270S, Y271L, N311G, C313T

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCSC-NLS-EGFP

    Plasmid

    #221811

    Purpose

    Lentiviral vector expressing green fluorescent protein (GFP) fused with nuclear localization sequence (NLS)

    Depositor

    Baojin Ding

    Article

    Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

    Insert

    NLS-EGFP
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. slc1a3b:myrGFP-P2A-H2AmCherry

    Plasmid

    #170208

    Purpose

    Drives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleus

    Depositor

    Kelly Monk

    Article

    Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

    Insert

    slc1a3b (slc1a3b Zebrafish)
    

    Use

    Zebrafish

    Tags

    myrGFP-P2A-H2AmCherry

    Promoter

    slc1a3b

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. mADAM17 in pcDNA3.1

    Plasmid

    #19141

    Depositor

    Zena Werb

    Article

    Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

    Insert

    ADAM17 (Adam17 Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

19. MSCV-IRES-NRASG12D-BFP

    Plasmid

    #236202

    Purpose

    Expresses NRASG12D in mammalian cells

    Depositor

    Michael Heuser

    Article

    Mohanty et al Blood Adv. 2025 May 7:bloodadvances.2024

    Insert

    NRAS (NRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    MSCV

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV3TAG8li-hRNF160-3FLAG

    Plasmid

    #159138

    Purpose

    human RNF160 (LTN1, ZNF294) FLAG mammalian expression vector.

    Depositor

    Martin Dorf

    Article

    Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

    Insert

    listerin E3 ubiquitin protein ligase 1 (LTN1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAR18_StrepI_mFASm_H8_pET22b

    Plasmid

    #122847

    Purpose

    Expresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    mouse FASN/ mouse fatty acid synthase (Fasn Mouse)
    

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3-RIIα-Dronpa

    Plasmid

    #182331

    Purpose

    PKA RIIα subunit-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    RIIalpha-Dronpa (PRKAR2A Human)
    

    Tags

    Full-length human PKA type 2alpha regulatory subu…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pGEX6P1-mCherry-Nanobody (LaM-2)

    Plasmid

    #162276

    Purpose

    Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

    Insert

    mCherry-Nanobody (LaM-2)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pINDUCER20_3xFlag

    Plasmid

    #190028

    Purpose

    Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal 3xFlag

    Depositor

    Andrew Tutt

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. Myc-E-Syt1

    Plasmid

    #66833

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt1 (ESYT1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. lenti.Cas9.BFP.Blast

    Plasmid

    #196714

    Purpose

    WT SpCas9 with BSD-TagBFP for selection and sorting

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pYMA3_SidK_GFP

    Plasmid

    #184276

    Purpose

    Legionella pneumophila SidK (1-278) - GFP

    Depositor

    John Rubinstein

    Article

    Maxson et al J Cell Biol. 2022 Mar 7;221(3). pii: 212

    Insert

    SidK (1-278) (sidK Legionella pneumophila)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pHR-CD80

    Plasmid

    #223603

    Purpose

    Lentiviral expression of human CD80 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD80 (CD80 Human)
    

    Use

    Lentiviral; mammalian expression

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. Flag-GFP-HRP-TM_pLX304

    Plasmid

    #202021

    Purpose

    expresses GFP-HRP anchored to the extracellular side of the plasma membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    Flag-GFP-HRP-TM
    

    Use

    Lentiviral

    Tags

    Flag and GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCgCas9_recT

    Plasmid

    #153337

    Purpose

    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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