Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

163,606 results

Showing: 8461 - 8490 of 163606 results

  1. BII-ChPtW-iRFP670

    Plasmid
    #133397
    Purpose
    Piggybac vector for constitutive expression of iRFP670-NLS
    Depositor
    Blair Madison
    Insert
    iRFP-670-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    133397-plasmid-map-sequence-id-269430

    Enlarge

  4. pNIC28-SENP2

    Plasmid
    #196355
    Purpose
    protein expression of the His-tagged catalytic domain
    Depositor
    Stefan Knapp
    Insert
    SENP2 catalytic domain
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    contains only D364-L589
    Promoter
    T7
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196355-plasmid-map-sequence-id-392708

    Enlarge

  6. pX330-BsaIx2-DD-Cas9

    Plasmid
    #75380
    Purpose
    Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1
    Depositor
    Michele Calos
    Article
    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv
    Insert
    DD-SpCas9
    Use
    CRISPR
    Tags
    FKBP12 L106P DD
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    75380-plasmid-map-sequence-id-192102

    Enlarge

  7. pLEX307-CTSB-blast

    Plasmid
    #158464
    Purpose
    Constitutive lentiviral expression of human CTSB.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    CTSB (CTSB Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158464-plasmid-map-sequence-id-308167

    Enlarge

  8. pCMV-enCas12a-A3A-Y130F

    Plasmid
    #193652
    Purpose
    Expression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    dLb-enCas12a-hA3A-Y130F, EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130F
    Promoter
    CMV
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193652-plasmid-map-sequence-id-422135

    Enlarge

  13. pRSV-PKImut-v2

    Plasmid
    #45067
    Purpose
    Expression of intactive PKI (PKA inhibitor) mutant
    Depositor
    Richard Maurer
    Insert
    cAMP-dependent protein kinase inhibitor alpha
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Arg 20 & 21 to Glycines
    Promoter
    RSV
    Available Since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    45067-plasmid-map-sequence-id-62502

    Enlarge

  16. pGEX-6P-1-GST-CD(Cbx7)

    Plasmid
    #82525
    Purpose
    Expresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 chromodomain only; amino acids 1–62.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    CD(Cbx7) ; amino acids 1–62
    Promoter
    Available Since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82525-plasmid-map-sequence-id-156818

    Enlarge

  19. pSLQ10844

    Plasmid
    #183956
    Purpose
    CAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, Hygromycin Resistance, and mCherry
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    183956-plasmid-map-sequence-id-363081

    Enlarge

  22. pAAV-EF1a-DIO-hM3D(Gq)-mCherry

    Plasmid
    #50460
    Purpose
    Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoter
    Depositor
    Bryan Roth
    Insert
    hM3D(Gq)-mCherry (CHRM3 Human)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    EF1a
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50460-plasmid-map-sequence-id-182307

    Enlarge

  28. pLenti-C-Myc-DDK-IRES-Puro LARP7

    Plasmid
    #200725
    Purpose
    Lentiviral expression of Flag-Tagged human LARP7
    Depositor
    Claudio Alarcón
    Insert
    LARP7 (LARP7 Human)
    Use
    Lentiviral
    Tags
    Myc-DDK from backbone plasmid
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200725-plasmid-map-sequence-id-403779

    Enlarge

Showing: 8461 - 8490 of 163606 results