We narrowed to 7,101 results for: tev

1. pMal-Abl-PRM 5R

   Plasmid

   #112088

   Purpose

   Bacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   PRM-5R (ABL1 Human)
   

   Use

   Tags

   His-6 and MBP

   Expression

   Bacterial

   Mutation

   construct contains only the PRM motif of Abl1

   Promoter

   Lac

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)

   Plasmid

   #217767

   Purpose

   Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
   

   Use

   Protein purification (6xhis tag, tev site, sumo3 …

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   CA3 domain aa 317-400

   Promoter

   T7 promoter

   Available since

   June 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

   Plasmid

   #217766

   Purpose

   Fluorescent reporter for ceramide (for bacterial expression and purification)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
   

   Use

   Protein purification (6xhis tag, tev site, sumo3 …

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   CA3 domain aa 317-400 plus vector-based linker LE…

   Promoter

   T7 promoter

   Available since

   June 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. TH0901-pOCC177-FUS-wt_opt(Nhe)

   Plasmid

   #221885

   Purpose

   Shuttle vector for baculovirus production, using FlashBac bacmid

   Depositor

   Anthony Hyman

   Article

   Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   6xHis-MBP-PreScission and TEV-SNAP-tag

   Expression

   Insect

   Mutation

   wt

   Promoter

   PH promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 6MIW

   Plasmid

   #127291

   Purpose

   Bacterial expression for structure determination. May not contain entire coding region of gene

   Depositor

   Cheryl Arrowsmith

   Article

   Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   6xHis-TEV (MHHHHHHSSGRENLYFQG)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT

   Plasmid

   #187723

   Purpose

   Express His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 1-418+582-975 (NEDD4L Human)
   

   Use

   Tags

   His-TEV

   Expression

   Insect

   Mutation

   deletion of residues 419-581

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBacHTA-NEDD4L_W1-L-W2-HECT

   Plasmid

   #187724

   Purpose

   Express His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 153-418+582-975 (NEDD4L Human)
   

   Use

   Tags

   His-TEV

   Expression

   Insect

   Mutation

   deletion of residues 1-152 and 419-581

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastBac dual_Human full length _CDK7 (S164D, T170E)_del327-346

   Plasmid

   #217012

   Purpose

   Expression of CDK7 in insect cells

   Depositor

   Manjeet Mukherjee

   Article

   Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

   Insert

   CDK7 (S164D, T170E)_del327-346 (CDK7 Human)
   

   Use

   Tags

   TEV cleavable his tag

   Expression

   Insect

   Mutation

   (S164D, T170E)_del327-346

   Promoter

   Available since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFastBac dual_Human full length CDK7(W132R, S164D, T170E)

   Plasmid

   #217013

   Purpose

   Expression of CDK7 in insect cells

   Depositor

   Manjeet Mukherjee

   Article

   Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

   Insert

   CDK7(W132R, S164D, T170E) (CDK7 Human)
   

   Use

   Tags

   TEV cleavable his tag

   Expression

   Insect

   Mutation

   CDK7(W132R, S164D, T170E)

   Promoter

   Available since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TH0994-pOCC177-Fus-wt_(1-211)

    Plasmid

    #221886

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    6xHis-MBP-PreScission and TEV-SNAP-tag

    Expression

    Insect

    Mutation

    PLD domain (aa 1-211)

    Promoter

    PH promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TH1148-pOCC177-FUS(Nhe)-PLD_YtoF

    Plasmid

    #221887

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    6xHis-MBP-PreScission and TEV-SNAP-tag

    Expression

    Insect

    Mutation

    In PLD all Tyr (Y) changed to Phe (F)

    Promoter

    PH promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TH1149-pOCC177-FUS(Nhe)-RGG_RtoK

    Plasmid

    #221888

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    6xHis-MBP-PreScission and TEV-SNAP-tag

    Expression

    Insect

    Mutation

    In RGG (aa 212-526) most of Arg (R) changed to Ly…

    Promoter

    PH promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-His6-en1dFnCas9GFP

    Plasmid

    #201950

    Purpose

    Expression of en1dFnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    Expression of en1dFnCas9-GFP in bacterial cells
    

    Use

    CRISPR

    Tags

    6x His, HA, NLS, and mEGFP

    Expression

    Bacterial

    Mutation

    E1369R on FnCas9

    Promoter

    T7

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-His6-en15dFnCas9GFP

    Plasmid

    #201951

    Purpose

    Expression of en15dFnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    Expression of en15dFnCas9-GFP in bacterial cells
    

    Use

    CRISPR

    Tags

    6x His, HA, NLS, and mEGFP

    Expression

    Bacterial

    Mutation

    E1603H on FnCas9

    Promoter

    T7

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a Cdk2ap1ΔN (MT2B2)-MutTER

    Plasmid

    #178035

    Purpose

    Expression vector for the purpose of protein purification.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Nonviral

    Tags

    6xHIS - Thrombin - MBP- TEV-TRS

    Expression

    Bacterial

    Mutation

    First N-Terminal 27aa are deleted and Changed T(8…

    Promoter

    T7LacI

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. DOT1L-CC3

    Plasmid

    #111754

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    DOT1L (DOT1L Human)
    

    Use

    Tags

    8xHis-Thrombin-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Contains coiled coil #3 (residues 613-661)

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. DOT1L-CC1

    Plasmid

    #111756

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    DOT1L (DOT1L Human)
    

    Use

    Tags

    8xHis-Thrombin-SUMO-TEV

    Expression

    Bacterial

    Mutation

    contains coiled coil #1 (residues 506-555)

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. DOT1L-CC2

    Plasmid

    #111755

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    DOT1L (DOT1L Human)
    

    Use

    Tags

    8xHis-Thrombin-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Contains coiled coil #2 (residues 560-608)

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pTH340-SUP45(1-242)-GalBD

    Plasmid

    #29740

    Depositor

    Tobias von der Haar

    Insert

    SUP45 (SUP45 Budding Yeast)
    

    Use

    Tags

    Gal Activation domain and Myc

    Expression

    Yeast

    Mutation

    Codons 1-242 of the yeast SUP45 gene only

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTH339-SUP45(1-138)-GalBD

    Plasmid

    #29739

    Depositor

    Tobias von der Haar

    Insert

    SUP45 (SUP45 Budding Yeast)
    

    Use

    Tags

    Gal Activation domain and Myc

    Expression

    Yeast

    Mutation

    Codons 1-138 of the yeast SUP45 gene only

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge