We narrowed to 53,425 results for: plasmid

1. SJZ5-HPH

   Plasmid

   #228258

   Purpose

   Template plasmid for yeast gene knockout, confers antibiotic resistance to hygromycin B

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   hph
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SSA1 S.c.

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNR4A1-donor

   Plasmid

   #112295

   Purpose

   CRISPR donor plasmid to tag human transcription factor NR4A1 with GFP

   Depositor

   Kevin White

   Insert

   NR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   hg38:12:52058840:C>T, hg38:12:52058930:A>C,…

   Promoter

   Available since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-GluA2-scFv-mNeon-KDEL

   Plasmid

   #230057

   Purpose

   AAV plasmid that expresses a a single chain variable fragment (scFv) for anti- GluA2 with a C-terminal mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA2 single chain variable fragment (scFv)
   

   Use

   AAV

   Tags

   ER retrieval motif (KDEL) and mNeon

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. SJZ9-amdS

   Plasmid

   #228261

   Purpose

   Template plasmid for yeast gene knockout, allows the use of acetamide as sole nitrogen source

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   amdS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ENO2 S.c.

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGFP Cas

   Plasmid

   #50729

   Purpose

   mammalian expression of GFP-Cas. Note-This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Insert

   Cas (Bcar1 Rat)
   

   Use

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRN1120

   Plasmid

   #101750

   Purpose

   Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.

   Depositor

   Rene Verwaal

   Article

   Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCJ1021

   Plasmid

   #228761

   Purpose

   Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

   Depositor

   Bradley Yoder

   Insert

   Ift88 gRNAs (Ift88 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBIS-GALkFLP(URA)

   Plasmid

   #49460

   Purpose

   overexpression of Flp step-arrest mutant in yeast, URA selection

   Depositor

   Marc Gartenberg

   Article

   Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

   Insert

   Flp(H305L) (flp Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H305L, ‘step-arrest’ mutant,

   Promoter

   GAL10

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSP-GM1

   Plasmid

   #64739

   Purpose

   Yeast expression plasmid

   Depositor

   Jens Nielsen

   Article

   Chen et al FEMS Yeast Res. 2012 Aug;12(5):598-607.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-GluA1-scFv-mNeon-KDEL

    Plasmid

    #230056

    Purpose

    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 tagged with mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA1 single chain variable fragment (scFv)
    

    Use

    AAV

    Tags

    ER retrieval motif (KDEL) and mNeon

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. 6xO/S luc

    Plasmid

    #69445

    Purpose

    detection/assessment of OCT4 and SOX2 transcription factor activity

    Depositor

    Lisa Dailey

    Insert

    minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLZ12Km2-P23R:TA:fflucRT

    Plasmid

    #88901

    Purpose

    Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

    Depositor

    Thomas Proft

    Article

    Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

    Insert

    toxin-antitoxin + Red-shifted firefly luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLZ12Km2-P23R:TA:ffluc

    Plasmid

    #88900

    Purpose

    Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

    Depositor

    Thomas Proft

    Article

    Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

    Insert

    toxin-antitoxin + firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJA252

    Plasmid

    #21512

    Depositor

    Julie Ahringer

    Article

    Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

    Insert

    pmex-5 (w/o ATG)
    

    Use

    Multisite gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid

    #218710

    Purpose

    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJAT21

    Plasmid

    #204309

    Purpose

    For generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthparts

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-GFPn
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJAT25

    Plasmid

    #204310

    Purpose

    For generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-GFPc
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGBW-m4134100

    Plasmid

    #152050

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHis-003

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. SJZ7-KAN

    Plasmid

    #228260

    Purpose

    Template plasmid for yeast gene knockout, confers antibiotic resistance to G418

    Depositor

    Zhiping Xie

    Article

    Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

    Insert

    kan
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPM1 S.c.

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. SJZ4-NAT

    Plasmid

    #228257

    Purpose

    Template plasmid for yeast gene knockout, confers antibiotic resistance to nourseothricin

    Depositor

    Zhiping Xie

    Article

    Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

    Insert

    nat1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    FBA1 S.c.

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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