We narrowed to 8,688 results for: APP

1. pGG-9.47_VP1,3 #2515

   Plasmid

   #74237

   Purpose

   Modified plasmid with a different backbone that yields higher of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47

   Depositor

   Miguel Sena-Esteves

   Article

   Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

   Inserts

   AAV2 Rep
   AAV9.47 VP1
   AAV9.47 VP3
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-lox-3xSTOP-lox (JDW 869)

   Plasmid

   #224521

   Purpose

   A Gateway compatible middle entry clone containing a loxP flanked SV40 polyA, bGH polyA, and SV40 pA stop cassette

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LoxP-3xStop-LoxP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. ROSA26(MultiFPsΔPuro)

   Plasmid

   #140759

   Purpose

   Targeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).

   Depositor

   Hideyuki Okano

   Article

   Serizawa et al Development. 2019 Nov 4;146(21). pii: de

   Inserts

   puromycin-N-acetyltransferase
   Venus
   mCherry
   mCerulean
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CAGGS

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV3Tag8li-hMBOAT7-3xFLAG

   Plasmid

   #165474

   Purpose

   Human MBOAT7 mammalian expression vector with 3 C-terminal FLAG tags.

   Depositor

   Martin Dorf

   Article

   Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

   Insert

   Membrane bound O-acyltransferase domain containing 7 (MBOAT7 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PD-1-miSFIT-1x

   Plasmid

   #124679

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-1 (PDCD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO hChR2(E123A)-EYFP

   Plasmid

   #35507

   Purpose

   AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123A

   Promoter

   Ef1a

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAX198

   Plasmid

   #173042

   Purpose

   Custom vector for sgRNA library construction

   Depositor

   Brittany Adamson

   Article

   Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

   Insert

   HBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CaMKIIa-hChR2(E123A)-mCherry

   Plasmid

   #35506

   Purpose

   AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Has Service

   AAV9

   Insert

   hChR2
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E123A

   Promoter

   CaMKIIa

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CamKII-ArchT-GFP (PV2527)

   Plasmid

   #99039

   Purpose

   AAV-mediated expression of ArchT under the CamKII promoter

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   ArchT-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV PIG C/EBPa

    Plasmid

    #66985

    Purpose

    Retroviral expression plasmid for mouse C/EBPa expression; containing bicistronic IRES-GFP

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    C/EBP alpha (Cebpa Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    LTR

    Available since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-STIM1-Venus-173-C

    Plasmid

    #87619

    Purpose

    Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    STIM1-Venus173-C (STIM1 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CaMKIIa-hChR2(E123A)-EYFP

    Plasmid

    #35505

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Has Service

    AAV9

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-myc-CCR5

    Plasmid

    #98948

    Purpose

    Mammalian expression plasmid for N-terminal myc-tagged human CCR5

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CCR5 (CCR5 Human)
    

    Use

    Tags

    Signal/leader sequence from Influenza A virus HA …

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA4.TO-ORF45-2xCSTREP

    Plasmid

    #136206

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF45

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF45
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME-mScarlet-I-P2A-iCre (JDW 917)

    Plasmid

    #224516

    Purpose

    A Gateway-compatible middle entry clone containing mScarlet-I P2A-iCre (codon optimized)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mScarlet-I-P2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEBB HA CCDC22

    Plasmid

    #74903

    Purpose

    Express CCDC22 in mammalian cells

    Depositor

    Ezra Burstein

    Article

    Starokadomskyy et al J Clin Invest. 2013 May;123(5):2244-56.

    Insert

    CCDC22 (CCDC22 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha promoter

    Available since

    June 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e

    Plasmid

    #90010

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3e

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Cdc25C WT
    NBirA*-14-3-3e
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. human CD40 promoter

    Plasmid

    #53433

    Purpose

    promoter activity study

    Depositor

    Etty Benveniste

    Article

    Nguyen et al J Biol Chem. 2002 Apr 19;277(16):13796-8

    Insert

    CD40 promoter (CD40 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CD40

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. phage - ubc - nls - 2xmcp - egfp - BirA*

    Plasmid

    #131132

    Purpose

    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

    Depositor

    Ralf Jansen

    Article

    Mukherjee et al Proc Natl Acad Sci U S A. 2019 Jun 25;11

    Insert

    NLS-HA-2xMCP-EGFP-BirA*
    

    Use

    Lentiviral

    Tags

    NLS, HA

    Expression

    Mammalian

    Mutation

    BirA* = BIrA >R118G

    Promoter

    human ubiquitin C (UBC)

    Available since

    Aug. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-Lifeact-EGFP-3xHA (JDW 1322)

    Plasmid

    #224494

    Purpose

    Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Liefact-EGFP-3xHA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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