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pCMV-R-Kras

Plasmid

#180572

Purpose

ddRFP sensor of Kras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Kras

Expression

Mammalian

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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PB-T3G-CERP2

Plasmid

#175498

Purpose

All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pFM571

Plasmid

#74634

Purpose

alias pFA6a-mCherry-kanMX6 - vector for C-terminal tagging of yeast genes with mCherry and G418 selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

mCherry

Promoter

none

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pT7-M2-S2-S3-S4T3D

Plasmid

#33302

Depositor

Terence Dermody

Article

Kobayashi et al Virology. 2010 Mar 15;398(2):194-200. Ep

Inserts

S2 (S2 Orthoreovirus T3D)
S3 (S3 Orthoreovirus T3D)
S4 (S4 Orthoreovirus T3D)
M2 (M2 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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MTK4_003

Plasmid

#123812

Purpose

Encodes rabbit beta-globin polyadenylation signal as a Type 4 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

rglb pA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pPD559 ZF173x6-Compact_EYFP

Plasmid

#138873

Purpose

EYFP reporter vector for the ZF173x6-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF173x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF173x6-Compact

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPD539 VP16-ZF173

Plasmid

#138773

Purpose

Constitutive expression of VP16-ZF173 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF173

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD526 VP16-ZF13

Plasmid

#138755

Purpose

Constitutive expression of VP16-ZF13 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF13

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-AsCas12f-WT

Plasmid

#204635

Purpose

Expression of wild-type AsCas12f in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

AsCas12f

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-07

Plasmid

#124786

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pHCMV_CHHF_GPC

Plasmid

#207260

Purpose

Mammalian expression of Chapare hemorrhagic fever virus GPC protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

CHHF_GPC (CVsS_gp1 Chapare hemorrhagic fever virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMVMagFRET-1-NLS

Plasmid

#50748

Purpose

Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.

Depositor

Maarten Merkx

Article

Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

Insert

gene encoding for MagFRET-1 with NLS

Expression

Mammalian

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pPD937 ZF37x12-Compact_EYFP

Plasmid

#138932

Purpose

EYFP reporter vector for the ZF37x12-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF37x12-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF37x12-Compact

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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Coronin1B-pmCherryN1

Plasmid

#27694

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Coronin 1B (Coro1b Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
pHCMV_SLEV_PrME

Plasmid

#207309

Purpose

Mammalian expression of St. Louis encephalitis virus PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

SLEV_PrME (POLY St. Louis encephalitis virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_MVEV_PrME

Plasmid

#207295

Purpose

Mammalian expression of Murray valley encephalitis virus PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

MVEV_PrME

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pPD128 AND Gate, 3 repeats

Plasmid

#138937

Purpose

EYFP reporter vector for the Logic 37 AND 158 NOT 43 3 repeats promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

Logic 37 AND 158 NOT 43 3 repeats

Use

Synthetic Biology

Expression

Mammalian

Promoter

Logic 37 AND 158 NOT 43 3 repeats

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPD296 ZF43x12-Compact_EYFP

Plasmid

#138907

Purpose

EYFP reporter vector for the ZF43x12-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x12-Compact

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-W340A.pcDNA3-k

Plasmid

#127404

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon and W34…

Promoter

CMV

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pPD290 ZF43x6-Compact_EYFP

Plasmid

#138901

Purpose

EYFP reporter vector for the ZF43x6-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x6-Compact

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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