We narrowed to 11,743 results for: pcdna

1. pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

   Plasmid

   #112157

   Purpose

   Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.

   Depositor

   Ben Giepmans

   Article

   de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261

   Insert

   anti-GFP FLIPPER-body
   

   Use

   Tags

   His and Thrombin cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5 frt/to N-BioTAP-C-BRD4-NUT

   Plasmid

   #171630

   Purpose

   Expresses BioTAP-tagged BRD4-NUT in mammalian cells. Can be inserted into a FRT site by co-transfection with a plasmid encoding Flp recombinaase.

   Depositor

   Chris French, Christopher A French

   Article

   Alekseyenko et al Proc Natl Acad Sci U S A. 2017 May 23;11

   Insert

   BRD4-NUT (BRD4, NUTM1 Human)
   

   Use

   Tags

   BioTAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO_Vim-SNAP-tag2

   Plasmid

   #226518

   Purpose

   Mamalian cell expression of intermediate filaments (vimentin) fused to SNAP-tag2

   Depositor

   Kai Johnsson

   Article

   Kühn et al bioRxiv 2024.08.28.610127

   Insert

   Vimentin-SNAP-tag2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1319 pcDNA3 flag FKHRL1 AAA

   Plasmid

   #10709

   Depositor

   William Sellers

   Article

   Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

   Insert

   FKHRL1 AAA (FOXO3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   AAA mutant (see map) - T32A, S253A, S315A

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 T21R-vhhGFP4-T2A-mCherry

   Plasmid

   #225587

   Purpose

   Mammalian expression vector for TRIM21 RING-vhhGFP4 anti-GFP degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-vhhGFP4-T2A-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-mouse PKA-RIalpha-mEGFP

   Plasmid

   #45525

   Depositor

   Haining Zhong

   Article

   Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

   Insert

   PKA regulatory subunit RI alpha tagged by mEGFP
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.2/DEST/hCx43-E2HA-stop

   Plasmid

   #40909

   Depositor

   Robin Shaw

   Article

   Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

   Insert

   Connexin 43 internal HA tag (GJA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HA tag inserted in second extracellular loop

   Promoter

   CMV

   Available since

   Nov. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

8. Nano-lantern(cAMP-1.6)/pcDNA3

   Plasmid

   #53594

   Purpose

   Nano-lantern-based luminescent cAMP indicator

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern-based luminescent cAMP indicator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pCDNA3.1-Flag-H2A K13-15R

   Plasmid

   #63563

   Purpose

   Expression of human H2A with K13R and K15R tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysine 13 and Lysine 15 are now Arginine

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)

    Plasmid

    #158953

    Purpose

    Express human RAD51(T309A) mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    RAD51 (RAD51 Human)
    

    Use

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    changed threonine 309 to alanine

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Lyn-dPlcR(K30/32L)

    Plasmid

    #181833

    Purpose

    Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid.

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-dPlcR (K30L,K32L)
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase, dd…

    Expression

    Mammalian

    Mutation

    Lysines 30 and 32 in PH domain mutated to leucine.

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C58

    Plasmid

    #171428

    Purpose

    Expression of FKBP-(GGS)X6-HaloTag C58 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    FKBP-(GGS)X6-HaloTag C58
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-LoxP-mKate-LoxP-EGFP

    Plasmid

    #167940

    Purpose

    CMV promoter-LoxP-mKate-LoxP-EGFP

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    LoxP-mKate-LoxP-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-HaloTag N58-(GGS)X6-c-Jun

    Plasmid

    #171438

    Purpose

    Expression of HaloTag N58-(GGS)X6-c-Jun in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-c-Jun
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA 3.1(-) mouse C/EBP delta

    Plasmid

    #12559

    Depositor

    Peter Johnson

    Article

    McCauslin et al J Biol Chem. 2006 Jun 30;281(26):17681-8

    Insert

    CCAAT/Enhancer-binding Protein delta (Cebpd Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S2G introduced during cloning

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3-myc-his-BACH1 P47A

    Plasmid

    #17644

    Depositor

    Ronny Drapkin

    Article

    Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

    Insert

    BACH1 (BRIP1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    To generate the tumor-associated mutant, the prol…

    Promoter

    Available since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

17. 1099 pcDNA GFP FKHR AAA

    Plasmid

    #9023

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR AAA (FOXO1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T24A S256A S319A

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-mNG-IRES-mScarlet-dPCP

    Plasmid

    #231090

    Purpose

    Bicistronic construct encoding mNG in combination with mScarlet-dPCP via an EMCV-IRES sequence.

    Depositor

    John Ngo

    Insert

    mNG-IRES-mScarlet-dPCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 Flag YFP hNKCC1 (NT17)

    Plasmid

    #49085

    Purpose

    Expresses human NKCC1 with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    262 silent mutations from native hNKCC1

    Promoter

    CMV

    Available since

    Nov. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-KozATG-dL5-2XG4S-actin

    Plasmid

    #73262

    Purpose

    Expresses dL5(E52D)-actin fusion within mammalian cells. (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.

    Insert

    KozATG-dL5-2XG4S-actin (ACTB Synthetic, Human)
    

    Use

    Tags

    The FAP and actin are fused with 2 copies of a G4…

    Expression

    Mammalian

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    CMV

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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