19,654 results

1. Cys-3HB-SPYcatcher

   Plasmid

   #214838

   Purpose

   This plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

   Insert

   Cys-3HB-SPYcatcher
   

   Tags

   His-TEV-SUMO-

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti CMVtight Puro DEST (w768-1)

   Plasmid

   #26430

   Depositor

   Eric Campeau

   Type

   Empty backbone

   Use

   Lentiviral; Tet-on advanced destination vector

   Expression

   Mammalian

   Available Since

   Dec. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET His6 GFP TEV LIC cloning vector (1GFP)

   Plasmid

   #29663

   Depositor

   Scott Gradia

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   6xHis, GFP, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCM433kanT

   Plasmid

   #61262

   Purpose

   Sucrose counterselection (sacB) vector for unmarked allelic exchange.

   Depositor

   Mary Lidstrom

   Article

   Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

   Type

   Empty backbone

   Use

   Synthetic Biology; Sucrose counterselection

   Expression

   Bacterial

   Available Since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWZL Neo Myr Flag YES1

   Plasmid

   #20665

   Depositor

   William Hahn, Jean Zhao

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   YES1 (YES1 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-mDlx-GFP-Fishell-1

   Plasmid

   #83900

   Purpose

   GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

   Depositor

   Gordon Fishell

   Article

   Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   eGFP
   

   Use

   AAV

   Promoter

   mDlx

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-Cas9-6xHis

   Plasmid

   #62374

   Purpose

   Expression of Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cas9_6xHis
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pL97

   Plasmid

   #78576

   Purpose

   Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoter

   Depositor

   Xuming Mao

   Article

   Sun et al Annals of Microbiology Dec 2012, Volume

   Type

   Empty backbone

   Use

   Streptomyces expression

   Expression

   Bacterial

   Promoter

   ermEp* promoter from S. erythraea

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. NbV5-eGFP

   Plasmid

   #201473

   Purpose

   Indirect microscopy/imaging of V5-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   NbV5-eGFP
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pL-CRISPR.EFS.GFP

    Plasmid

    #57818

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Promoter

    EFS and hU6

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX855

    Plasmid

    #62887

    Purpose

    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Aspartic acide 10 to Alanine (D10A)

    Promoter

    CBh

    Available Since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-mLin28A

    Plasmid

    #26357

    Depositor

    George Daley

    Article

    Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

    Insert

    lin-28 homolog A (Lin28a Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

13. Stat3-C Flag pRc/CMV

    Plasmid

    #8722

    Depositor

    Jim Darnell

    Article

    Bromberg et al Cell 1999 Aug 6;98(3):295-303.

    Insert

    Stat3-C (Stat3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    A662C and N664C. Residues changed to Cys renders…

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. Twinkle-APEX2-V5

    Plasmid

    #129705

    Purpose

    APEX2 fused to the mitochondrial nucleoid protein Twinkle

    Depositor

    Alice Ting

    Article

    Han et al Cell Chem Biol. 2017 Mar 16;24(3):404-41

    Insert

    Twinkle-APEX2-V5 (TWNK, APEX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pArcCreER

    Plasmid

    #46389

    Depositor

    Liqun Luo

    Article

    Guenthner et al Neuron. 2013 Jun 5;78(5):773-84. doi: 10

    Inserts

    Arc (Arc Mouse)
    CreERT2
    

    Use

    Mouse Targeting

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-TGNP-N-10

    Plasmid

    #55145

    Purpose

    Localization: Golgi Complex, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    TGNP (TGOLN2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. LifeAct-GFP-Fis1

    Plasmid

    #182576

    Purpose

    Expresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    LifeAct-GFP-Fis1
    

    Tags

    Fis1 and LifeAct

    Expression

    Mammalian

    Available Since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-CMV-GCaMP6s-CAAX

    Plasmid

    #52228

    Purpose

    Plasma membrane targeted GCaMP6s, calcium sensor

    Depositor

    Tobias Meyer

    Article

    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

    Insert

    GCaMP6s-CAAX
    

    Tags

    6xHis and CAAX

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3 3 kb prom. CD274

    Plasmid

    #107004

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAIDA1

    Plasmid

    #79180

    Purpose

    A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags

    Depositor

    Gen Larsson

    Article

    Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi

    Insert

    Adhesin Involved in Diffuse Adherence
    

    Tags

    His and Myc

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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