We narrowed to 22,253 results for: his

1. pEBTet-SNAP-Cep76

   Plasmid

   #136813

   Purpose

   Mammalian expression of the centrosomal protein Cep76 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep76 (CEP76 Synthetic, Human)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pet23a_RBPMS_FL

   Plasmid

   #59392

   Purpose

   Plasmid for bacterial overexpression of RBPMS

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-PKM2-G415R

   Plasmid

   #122693

   Purpose

   Express His-tagged PKM2 (G415R) protein in bacteria.

   Depositor

   Wen-Ching Wang

   Article

   Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

   Insert

   Pyruvate kinase M2 isoform (PKM Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   G415R

   Promoter

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. CaV1.3e[8a,11,31b,Δ32,Δ42a] mut

   Plasmid

   #26577

   Depositor

   Diane Lipscombe

   Article

   Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

   Insert

   CACNA1D (Cacna1d Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

   Promoter

   Available Since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEBTet-SNAP-Cep41

   Plasmid

   #136794

   Purpose

   Mammalian expression of the centrosomal protein Cep41 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep41 (CEP41 Synthetic, Human)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEBTet-SNAP-α-tubulin

   Plasmid

   #136791

   Purpose

   Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-TUBA (DNMBP Synthetic, Human)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hHSF1 S303A

   Plasmid

   #118363

   Purpose

   This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   S303A

   Promoter

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p53-(1-393)-E285K-N268D

   Plasmid

   #35089

   Depositor

   Cheryl Arrowsmith

   Article

   Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Contains aa 1-393 with N269D and E285K mutations

   Promoter

   T7

   Available Since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

   Plasmid

   #101231

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S701C/S960C/N692A/M694A/Q695A/H698A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S701C, S960C, N692A, M694A, Q695A an…

   Promoter

   T7

   Available Since

   Nov. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Dom34(full length)

    Plasmid

    #19098

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

11. SPRR2A-pFastBac I

    Plasmid

    #182460

    Purpose

    Expression His-tagged SPRR2A in insect cells

    Depositor

    Lora Hooper

    Article

    Hu et al Science. 2021 Nov 5;374(6568):eabe6723.

    Insert

    small proline rich protein 2A (SPRR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. LV_EF1a_Stat3(A662C,N664C,V667L)-P2A-Hygro_Barcode

    Plasmid

    #170254

    Purpose

    Barcoded lentiviral vector to express Stat3 (A662C, N664C, V667L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    A662C, N664C, V667L

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEBTet-SNAP-γ-tubulin

    Plasmid

    #136799

    Purpose

    Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-TUBG (TUBG1 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET20b-delta133HtrA2 A141S

    Plasmid

    #16157

    Depositor

    L. Miguel Martins

    Article

    Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    A141S

    Promoter

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHM053Env

    Plasmid

    #139019

    Purpose

    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

    Depositor

    Anjali Joshi

    Article

    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

    Insert

    HIV-1 Envelope Glycoprotein (env HIV-1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MBP-hnRNPA2_FL_P298L

    Plasmid

    #104468

    Purpose

    express MBP-tagged full length hnRNPA2 P298L

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    P298L

    Promoter

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. human TLNRD1-F250D pET151

    Plasmid

    #159385

    Purpose

    Expresses human TLNRD1 F250D mutant in bacterial cells

    Depositor

    Ben Goult

    Article

    Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    Talin Rod Domain Containing Protein 1 F250D mutant (TLNRD1 Human)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Changed Phenylalanine 250 to Aspartic Acid (F250D)

    Promoter

    T7

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA Flag-RARalpha (T181A,S445A,S461A)

    Plasmid

    #35556

    Depositor

    Jonathan Kurie, Harish Srinivas

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    T181A, S445A, S461A

    Promoter

    Available Since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pASK/3xMBT

    Plasmid

    #28234

    Depositor

    Danny Reinberg

    Article

    Trojer et al Cell. 2007 Jun 1. 129(5):915-28.

    Insert

    l(3)mbt-like 1 (Drosophila) (L3MBTL1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation expressing only aa 252-606 (MBT repeat…

    Promoter

    Available Since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pEBTet-SNAP-Cep70

    Plasmid

    #136821

    Purpose

    Mammalian expression of the centrosomal protein Cep70 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep70 (CEP70 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET41a-MED18

    Plasmid

    #15433

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED18 (MED18 Human)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

22. pIE-C-hα2M-H6

    Plasmid

    #128494

    Purpose

    Expresses human C-α2M (from R787 to N1473) in insect cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (R787-N1473) (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Insect

    Mutation

    contains R787-N1473

    Promoter

    IE1

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PET28-FnCas12a-EP16

    Plasmid

    #183636

    Purpose

    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.

    Depositor

    Yunzi Luo

    Article

    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-

    Insert

    FnCas12a-EP16
    

    Use

    CRISPR

    Tags

    6×His Tag

    Expression

    Bacterial

    Mutation

    N607R, K613V, N617R, K180S, K660R, D616N

    Promoter

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pS6-C-hα2M-H6

    Plasmid

    #128497

    Purpose

    Expresses human C-α2M (from F789 to S1468) in mammalian cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (F789-S1468) (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEBTetDBl-CLIP-α-tubulin

    Plasmid

    #136841

    Purpose

    Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-TUBA (DNMBP Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HOXB4 (human) HA-TAT-tag pET

    Plasmid

    #8526

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    replace T7 and HIS tags of pET with an HA tag;

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

27. pEBTetBl-CLIP-Cep78

    Plasmid

    #136876

    Purpose

    Mammalian expression of the centrosomal protein Cep78 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep78 (CEP78 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28b-API5

    Plasmid

    #157655

    Purpose

    Expresses API5 in E.coli cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Apoptosis inhibitor 5 (API5 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Delta DED FLIP-L D/A

    Plasmid

    #13791

    Depositor

    Guy Salvesen

    Article

    Boatright et al Biochem J. 2004 Sep 1. 382(Pt 2):651-7.

    Insert

    DELTA DED FLIP LONG (CFLAR Human)
    

    Use

    Tags

    8 x His tag

    Expression

    Bacterial

    Mutation

    Delta DED removes first 186 residues Asp to Ala m…

    Promoter

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mApple-M1 SpastinHFDD

    Plasmid

    #134462

    Purpose

    M1 SpastinHFDD expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Met to Ala at amino acid 87; His to Asp at amino …

    Promoter

    CMV

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. nuc-yHS1-M7A,H102A

    Plasmid

    #159168

    Purpose

    Nuclear labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    nuc-yHS1-M7A,H102A
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Mutated both Met 7 and His 102 of the cyt b562 mo…

    Promoter

    GPD

    Available Since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. mito-yHS1-M7A,H102A

    Plasmid

    #159165

    Purpose

    Mitochondrial labile heme reporter

    Depositor

    Amit Reddi

    Article

    Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

    Insert

    mito-yHS1-M7A,H102A
    

    Use

    Tags

    COX4 pre-sequence

    Expression

    Yeast

    Mutation

    Mutated both Met 7 and His 102 of the cyt b562 mo…

    Promoter

    GPD

    Available Since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pEBTet-SNAP-Cep27

    Plasmid

    #136808

    Purpose

    Mammalian expression of the centrosomal protein Cep27 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep27 (HAUS2 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28b-Rv2416c_wild

    Plasmid

    #37649

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

    Insert

    Rv2416c (eis Mycobacterium tuberculosis)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

35. pET28a-PTBP1-RRM12CCtoSS

    Plasmid

    #89155

    Purpose

    Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
    

    Use

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET21 Catalytic Domain of MALT1 Catalytic Mutant

    Plasmid

    #48971

    Purpose

    Expresses catalytic domain of MALT1 catalytic mutant in e.coli AA 329-566

    Depositor

    Guy Salvesen

    Article

    Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

    Insert

    MALT1 (MALT1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains AA 329-566; Catalytic Mutant C464A

    Promoter

    T7

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

37. pJ136

    Plasmid

    #29486

    Depositor

    Jonathan Tait

    Article

    Jin et al J Biol Chem 2004; 279:40351-57.

    Insert

    annexin V-128 (ANXA5 Human)
    

    Use

    Tags

    (Met)-Ala-Gly-Gly-Cys-Gly-His

    Expression

    Bacterial

    Mutation

    Deletion of the initiator Met at position 1 of wi…

    Promoter

    Available Since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

38. pet23a_RBPMS_1_101

    Plasmid

    #65728

    Purpose

    plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    deletion construct encoding amino acids 1-101

    Promoter

    T7

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)

    Plasmid

    #61938

    Purpose

    Co-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF2) (UCHL5 Human)
    hNFRKB (39-156) (NFRKB Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 156, residues 2-38 dele…

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

40. pEJS663-pMCSG7-AcrIIC4Hpa

    Plasmid

    #113440

    Purpose

    Expresses a 6X-His tagged type II-C anti-CRISPR protein from H. parainfluenzae in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    Type II-C anti-CRISPR with N-terminal 6xHis and TEV site
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    lacI

    Available Since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge