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FUP95Q15AGW (D5)

Plasmid

#74028

Purpose

lentiviral expression of Psd95-Q15A-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95 (DLG4 Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

Q15A

Promoter

hUbC

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only
FUP95E17RGW (D6)

Plasmid

#74029

Purpose

lentiviral expression of Psd95-E17R-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95 (DLG4 Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

E17R

Promoter

hUbC

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
FUP95DSKGW (C8)

Plasmid

#74024

Purpose

lentiviral expression of Psd95-delta SK-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95 (DLG4 Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

delta SK

Promoter

hUbC

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
tdEos-Cx43-7

Plasmid

#57603

Purpose

Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Cx43

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pFL-TK-AID3'UTR-DEL

Plasmid

#19988

Depositor

Nina Papavasiliou

Article

Teng et al Immunity. 2008 May . 28(5):621-9.

Insert

AID 3' UTR (Aicda Mouse)

Expression

Mammalian

Mutation

Deletion

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459) V2.0

Plasmid

#62988

Purpose

Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Has Service

Cloning Grade DNA

Insert

hSpCas9-2A-Puro V2.0

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP - FLAG-APEX2- hs Lamin B1

Plasmid

#139442

Purpose

APEX2-Lamin B1 expression plasmid

Depositor

Yixian Zheng

Article

Tran et al bioRxiv 2020.02.05.935635

Insert

LMNB1 (LMNB1 Human)

Tags

3xGGGGS linker (between APEX2 and Lamin B1), APEX…

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Cas9-pA-Cry-GFP

Plasmid

#168239

Purpose

"neutrophil specific cas9 expression in zebrafish with green lens"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Cas9

Use

Zebrafish expression

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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lenti dCAS-VP64_Blast

Plasmid

#61425

Purpose

3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Lentiviral Prep

Insert

dCAS9(D10A, N863A)-VP64_2A_Blast

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1A

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pDonor_e-attP-H1-g3-Ef1a-Puro-P2A-mCherry

Plasmid

#247160

Purpose

Engineered e-attP donor plasmid expressing puromycin resistance and mCherry with donor binding sgRNA H1-g3

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Puromycin resistance, mCherry

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pTmpl-miniDonor-GFP

Plasmid

#238107

Purpose

Mini donor expressing GFP, used for PCR to generate IVT template.

Depositor

Feng Zhang

Article

Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

Insert

CMV-GFP-syntheticPA flanked by R2Tg UTRs and 28S sequence

Expression

Mammalian

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pJRH-1363 LV EF1a-CD19 IRES-EGFP

Plasmid

#201919

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD19

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD19 (CD19 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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SuperClomeleon

Plasmid

#203724

Purpose

An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride

Depositor

George Augustine

Article

Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.

Insert

Cerulean linked topaz fluorescent protein

Expression

Bacterial and Mammalian

Mutation

Cerulean, modified Topaz Fluorescent Protein with…

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-flag-YTHDF2

Plasmid

#52300

Purpose

full length YTHDF2 with N-terminal flag tag for mammalian cell expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

flag

Expression

Mammalian

Promoter

T7

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Tet-UPF1WT (pRKB264)

Plasmid

#99146

Purpose

Inducible lentiviral construct to express FLAG-tagged UPF1wt

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

UPF1 (UPF1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

First 127 codons are optimized to reduce GC conte…

Promoter

pTight

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEGIP

Plasmid

#26777

Purpose

Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

EF1a-GFP-IRES-Puro (gfp )

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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anti-FLAG M2 heavy chain

Plasmid

#175359

Purpose

Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

Depositor

Joost Snijder

Article

Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

Insert

anti-FLAG M2 heavy chain

Tags

His8 tag and N-terminal secretion signal peptide …

Expression

Mammalian

Mutation

L51I (see paper)

Promoter

CMV

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-hALDOA

Plasmid

#210809

Purpose

For transient transfection

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)

Plasmid

#154824

Purpose

(eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegans

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

Peft-3::wrmScarlet::tbb-2 UTR

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

Plasmid

#42335

Purpose

A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Has Service

Cloning Grade DNA

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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