We narrowed to 7,293 results for: tet

1. pAX2

   Plasmid

   #117398

   Purpose

   allelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switch

   Depositor

   Karen Guillemin

   Article

   Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDAC569

   Plasmid

   #195241

   Purpose

   Mammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
   

   Expression

   Mammalian

   Available Since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC18T-mini-Tn7T-hph-dCas9

   Plasmid

   #218371

   Purpose

   Expression of dCas9 for CRISPR-interference

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Insert

   dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PtetA

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNG6

   Plasmid

   #30990

   Depositor

   Adam Bogdanove, Daniel Voytas

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   repeat NG6
   

   Available Since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-CEP41_Halo

   Plasmid

   #135446

   Purpose

   Mamalian cell expression of HaloTag7 localized at the centrosome

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

   Insert

   CEP41-HaloTag7
   

   Tags

   CEP41

   Expression

   Mammalian

   Promoter

   CMV/TetO

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4/TO-cMYC

   Plasmid

   #216510

   Purpose

   Expresses MYC in human cells under a tetracycline/doxycycline-inducible promoter

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   cMYC
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. qMaLioffG

   Plasmid

   #212825

   Purpose

   green fluorescent FLIM indicator for endogenous ATP imaging

   Depositor

   Tetsuya Kitaguchi

   Article

   Arai et al bioRxiv 2023.08.29.555275

   Insert

   qMaLioffG
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2.aBTX

   Plasmid

   #122257

   Purpose

   Express membrane-tethered alpha-bungarotoxin in Danio rerio

   Depositor

   Loren Looger

   Article

   Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi

   Insert

   alpha-bungarotoxin
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKIR1.0

   Plasmid

   #86409

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Promoter

   AtRPS5A

   Available Since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ134B2.0

    Plasmid

    #167158

    Purpose

    Golden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU3

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJT119b

    Plasmid

    #50551

    Purpose

    Expresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoter

    Depositor

    Jeffrey Tabor

    Article

    Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

    Inserts

    CcaS
    CcaR
    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSC101-GFPmut2-mScarlet-I TAA

    Plasmid

    #208187

    Purpose

    nonsense mistranslation, TAA stop

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Expression

    Bacterial

    Mutation

    Insertion 6TAA*(stop) 7AGT(Ser)

    Promoter

    Ptet+dnaK P1

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBA562

    Plasmid

    #186237

    Purpose

    pTet-RfxCas13d crRNA entry vector

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLO99

    Plasmid

    #90219

    Purpose

    Complements mutations in Aspergillus nidulans gene lysB (AN5206) (mutants require lysine)

    Depositor

    Berl Oakley

    Article

    Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

    Insert

    ATET_03691
    

    Use

    Aspergillus

    Promoter

    pLac

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-H2B_Halo_T2A_EGFP

    Plasmid

    #135444

    Purpose

    Mamalian cell expression of nuclear HaloTag7 and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

    Insert

    H2B-HaloTag7 and EGFP
    

    Tags

    H2B

    Expression

    Mammalian

    Promoter

    CMV/TetO

    Available Since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKL09-GCaMP6f-mScarlet bb118

    Plasmid

    #158979

    Purpose

    Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    GCaMP6f-mScarlet fusion
    

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#100833

    Promoter

    Bba_J23118 (sigma 70)

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEutRwc

    Plasmid

    #216334

    Purpose

    An IPTG-inducible promoter controlling expression of eutR; PEutS, extended controlling expression of sfgfp

    Depositor

    Mark Styczynski

    Article

    McNerney et al ACS Synth Biol. 2020 Feb 21;9(2):402-411

    Inserts

    EutR
    Superfolder green fluorescent protein
    

    Expression

    Bacterial

    Available Since

    Dec. 23, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pFTK055

    Plasmid

    #171327

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    rTA2S-M2 (TetR-3xVP16) transcriptional activator
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSC101-GFPmut2-mScarlet-I

    Plasmid

    #208183

    Purpose

    mistranslation GFP and mScarlet-I positive control

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Expression

    Bacterial

    Promoter

    Ptet+dnaK P1

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-TRE-DIO-GCaMP6s

    Plasmid

    #166606

    Purpose

    Encodes Cre-dependent GCaMP6s under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    GCaMP6s
    

    Use

    AAV

    Promoter

    tetracycline response element

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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