We narrowed to 9,184 results for: Pol;

1. pAMV:nAChRgamma-eGFP

   Plasmid

   #29693

   Depositor

   Henry Lester

   Article

   Pantoja et al Biophys J. 2009 Jan . 96(1):226-37.

   Insert

   GFP tagged Mus musculus cholinergic receptor, nicotinic, gamma (Chrng Mouse)
   

   Tags

   AMV promotor and poly(A) tail

   Expression

   Bacterial

   Mutation

   eGFP inserted after position S380

   Available Since

   July 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOCC94

   Plasmid

   #118912

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
   

   Tags

   MBP, cleavable with 3C protease and eGFP-HIS6

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBBK69 pHomL-RPL18Bp-SPC42-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179053

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Scarlet(rfp), a marker for the spindle pole

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-SPC42-Link-Scarlet(rfp)
   

   Expression

   Yeast

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOCC254

   Plasmid

   #118889

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6-MBP, cleavable with 3C protease; mCherry, cl…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5 FRT/TO Plk4 KD ∆24-mCherry

   Plasmid

   #80272

   Purpose

   mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted

   Depositor

   Andrew Holland

   Article

   Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

   Insert

   Plk4 (PLK4 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   deletion of multi-phospho degron (amino acids 282…

   Promoter

   CMV

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac1-OSF-TRIM5alpha(human)(R332P)

   Plasmid

   #79037

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5alpha(human R332P) (TRIM5 Human)
   

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Mutation

   Changed Arg 332 to Pro

   Promoter

   polyhedrin

   Available Since

   March 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. LacI/109-pHG165c

   Plasmid

   #90058

   Purpose

   WT LacI polymorphism with T at position 109

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LacI/109
   

   Expression

   Bacterial

   Mutation

   109

   Promoter

   Iq

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMuLE ENTR 7SK-miR-30 L5-L4

   Plasmid

   #62119

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   RNAi; Mule gateway entry vector

   Expression

   Mammalian

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

9. Arp3_pLib

   Plasmid

   #173677

   Purpose

   Arp3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

   Depositor

   Roberto Dominguez

   Article

   Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

   Insert

   Arp3 (ACTR3 Human)
   

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)

    Plasmid

    #79031

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(African green monkey pygerythrus)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    March 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. psiCheck-ApoE3'UTR

    Plasmid

    #41846

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

    Insert

    ApoE 3'UTR (APOE Human)
    

    Use

    Luciferase

    Available Since

    Jan. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

12. AL08_pgRNA.DMD

    Plasmid

    #100293

    Purpose

    DMD intron 43-targeting gRNA expression plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    gRNA spacer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6 Polymerase III snRNA

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOCC177

    Plasmid

    #118886

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal SNAP tag, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease and SNAP tag…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOCC194

    Plasmid

    #118882

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal SNAP tag, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    

    Tags

    SNAP tag, cleavable with TEV protease, HIS6, clea…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOCC210

    Plasmid

    #118896

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal SNAP, cleavable with TEV and HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease and SNAP tag, cle…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOCC231

    Plasmid

    #118885

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease and mCitrine…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEXP5-NT-CuSeCat EE

    Plasmid

    #42569

    Depositor

    Ivan Korendovych

    Article

    Mack et al J Biol Inorg Chem. 2013 Apr;18(4):411-8.

    Insert

    mutated calmodulin gene
    

    Tags

    Polyhistidine (6×His) region, TEV recognition sit…

    Expression

    Bacterial

    Mutation

    changed Phenylalanine 92 to Glutamine acid, Valin…

    Promoter

    T7

    Available Since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMuLE ENTR 7SK-miR-30 L5-L2

    Plasmid

    #62120

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    RNAi; Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pfast bac-flag Fbxl3

    Plasmid

    #51894

    Purpose

    Backbone plasmid is pfastBacHTa from invitrogen

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    Fbxl3 (Fbxl21 Mouse)
    

    Tags

    FLAG

    Promoter

    polyhedrin promoter

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

20. AI-CN590

    Plasmid

    #62596

    Purpose

    KCTD13 N-terminus disruption by homolgy-directed repair

    Depositor

    Joshua Grimley

    Article

    Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

    Insert

    Cerulean-P2A-PuroR (KCTD13 Human)
    

    Use

    TALEN; Homology-directed repair donor

    Expression

    Mammalian

    Promoter

    hEF1alpha

    Available Since

    March 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. KHBD00712

    Plasmid

    #39741

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG18412 (ph-p Fly)
    

    Use

    Gateway donr vector

    Promoter

    none

    Available Since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

22. pCR197

    Plasmid

    #240643

    Purpose

    Expression of the tri-cistronic construct NES-AmNeonGreen-P2A-mTurquoise2-NLS-T2A-mScarlet-I-PTS1 in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymerase

    Depositor

    John Pringle

    Inserts

    AIPGENE865 promoter
    SP6 promoter
    AmNeonGreen
    mTurquoise2
    mScarlet-I
    

    Use

    Gene expression and genomic integration in fish

    Tags

    NES (from Exaiptasia diaphana MEK2), NLS (from SV…

    Mutation

    Codon-optimized for Exaiptasia diaphana, amino ac…

    Available Since

    Aug. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. pGS-BacA-21222-SIN3B-3xFLAG

    Plasmid

    #109214

    Purpose

    Encodes human full-length SIN3B with a C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

    Insert

    human full-length SIN3B (SIN3B Human)
    

    Tags

    3xFLAG

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    July 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAAV-EF1a-DIO-Ace-mNeon2-WPRE

    Plasmid

    #240056

    Purpose

    Cre dependent Green fluorescent, negative response-polarity voltage indicator for mammalian expression

    Depositor

    Mark Schnitzer

    Article

    Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

    Insert

    Ace-mNeon2
    

    Use

    AAV

    Promoter

    Ef1a

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pEW66

    Plasmid

    #232794

    Purpose

    COMPASS Fragment 1 (Cps60, Cps50, Cps35, Cps25, Cps15) in PBIG1a

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

    Insert

    COMPASS Fragment 1 (Cps60, Cps50, Cps35, Cps25, Cps15) (SWD1, BRE2, SWD2, SDC1, SHG1 Budding Yeast)
    

    Tags

    No tags

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCRII-dgat2 in situ probe

    Plasmid

    #232134

    Purpose

    zebrafish dgat2 anti-sense in situ probe, linearize with BamHI, T7 polymerase

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    dgat2 (dgat2 Zebrafish)
    

    Use

    In vitro transcription

    Promoter

    SP6/T7

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFastBac-GST-TBK1

    Plasmid

    #221331

    Purpose

    TBK1 protein overexpression in Sf9 insect cells

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    TBK1 (Tbk1 Mouse)
    

    Tags

    6xHis-TEV-2xGST-TEV

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. AA271

    Plasmid

    #216074

    Purpose

    Fragmid fragment: (CROPseq) guide expression; UMI-compatible

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    WPRE_v1; U6_v1; BsmBI_v0; BsmBI_v5; trRNA_v6 [Sp]; 3'LTR; bGHpolyA_v1
    

    Use

    Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. pUC57-RPLP15UTR-hRluc-A60

    Plasmid

    #182639

    Purpose

    Transcription template plasmid for RPLP1 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

    Depositor

    Stephen Floor

    Article

    Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

    Insert

    Renilla luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    The transcription is driven by a T7 promoter, pai…

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. AV13_pCAG.Cas9.gRNA.NT

    Plasmid

    #199260

    Purpose

    Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Inserts

    SpCas9 nuclease
    Non-targeting (NT) control gRNA
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG promoter and RNA polymerase III promoter for …

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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