We narrowed to 6,132 results for: pCas

Showing: 5721 - 5740 of 6132 results

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pGreenII 0229/ProRUBY:GOXL2-Citrine:NOSt

Plasmid

#175564

Purpose

pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-2

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

GOXL2

Tags

Citrine

Expression

Plant

Promoter

RUBY

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEGB3 alpha2 P35S:RDF:T35S (GB1508)

Plasmid

#160613

Purpose

TU for the constitutive expression of Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

RDF

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pJT140_GalL_cCDA1-VRERBE3

Plasmid

#145103

Purpose

Expressing base editor cCDA1-VRERBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-VRERBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

Promoter

GalL

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT176_GalL_eA3A-BE3

Plasmid

#145125

Purpose

Expressing base editor eA3A-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A(N57G)-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(N57G); spCas9(D10A)

Promoter

GalL

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT174_GalL_A3A-Y130F-BE3

Plasmid

#145123

Purpose

Expressing base editor A3A-Y130F-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A(Y130F)-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(Y130F); spCas9(D10A)

Promoter

GalL

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT173_GalL_A3A-R128A-BE3

Plasmid

#145122

Purpose

Expressing base editor A3A-R128A-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A(R128A)-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(R128A); spCas9(D10A)

Promoter

GalL

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT171_GalL_A3AΔ178-BE3

Plasmid

#145121

Purpose

Expressing base editor A3AΔ178-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3AΔ178-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(1-178AA); spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT170_GalL_A3AΔ182-BE3

Plasmid

#145120

Purpose

Expressing base editor A3AΔ182-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3AΔ182-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(1-182AA); spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT169_GalL_A3AΔ186-BE3

Plasmid

#145119

Purpose

Expressing base editor A3AΔ186-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3AΔ186-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(1-186AA); spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT122_GalL_cCDA1-VQRBE3

Plasmid

#145087

Purpose

Expressing base editor cCDA1-VQRBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-VQRBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A, D1135V, R1335Q, T1337R)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT113_GalL_cCDA1-xBE3

Plasmid

#145079

Purpose

Expressing base editor cCDA1-xBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-xBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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mei-S332 5.6Kb genomic DNA in Casper4

Plasmid

#112984

Purpose

P element vector for transposon insertion of mei-S332 5.6Kb genomic DNA into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

Insert

mei-S332 (Sgo) (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pEGB3omega1R Tnos:NptII:Pnos-SF (GB1181)

Plasmid

#75471

Purpose

Transcriptional unit for kanamycin resistance gene

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

NptII

Use

Synthetic Biology

Expression

Plant

Promoter

Agrobacterium tumefaciens Nopaline synthase promo…

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pX335_HR_Prnp_3

Plasmid

#78622

Purpose

pX335 vector encoding SpCas9n and a chimeric guide RNA targeting Prnp coding sequence

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

HR_Prnp_3 (Prnp Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-KANK3

Plasmid

#69238

Purpose

U6 driven SpCas9 sgRNA expression for KANK3 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

KANK3 sgRNA (KANK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Bxb1 attB pDonor (JO1529)

Plasmid

#248223

Purpose

Bxb1 attB pDonor with Nt.BbvcI site for cssDNA production

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

Bxb1 attB

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

None

Promoter

NA

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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BLBP-GFP

Plasmid

#63174

Purpose

Expressing GFP from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter

Depositor

Bart De Strooper

Article

Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77

Insert

BLBP (Fabp7 Mouse)

Expression

Mammalian

Promoter

1700bp upstream of BLBP gene

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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iCas

Plasmid

#84232

Purpose

Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

Depositor

Meng How Tan

Article

Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

Insert

Cas9

Tags

2A-OFP co-expression and ERT2-ERT2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas13-inactive M3nls

Plasmid

#157854

Purpose

Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A; METTL3 D395A

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pABE8e-NG-403Q-Nterm-AAV

Plasmid

#194651

Purpose

N-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

Depositor

David Liu

Article

Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

Insert

ABE8e-NG N-term

Use

AAV

Tags

BPNLS and NpuN spilt intein

Promoter

TNNT2

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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