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C232-E25: attB2r-mVenus-attB3

Plasmid

#162905

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mVenus fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Venus fluorescent protein

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E34: attB2r-iRFP670-attB3

Plasmid

#162908

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal iRFP670 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

far-red iRFP670 fluorescent protein

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E11: attB5r-mTFP1-nostop-attB1r

Plasmid

#162942

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mTFP1 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Teal fluorescent protein

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E19: attB4-mPol2p>-attB1r

Plasmid

#162971

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

murine RNA polymerase 2 promoter

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E29: attB5r-mKate2-nostop-attB1r

Plasmid

#162946

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mKate2 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Kate2 fluorescent protein

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E15: attB5r-mVenus-nostop-attB1r

Plasmid

#162943

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mVenus fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Venus fluorescent protein

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E26: attB4-SV40p>-attB1r

Plasmid

#162923

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the SV40 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

SV40 immediate early promoter

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E21: attB4-PGKp>-attB1r

Plasmid

#162921

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human PGK promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human pGK promoter

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E07: attB2r-HA-attB3

Plasmid

#162911

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal HA epitope tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

HA epitope tag

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E06: attB2r-FLAG-attB3

Plasmid

#162910

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal FLAG epitope tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

FLAG epitope tag

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E05: attB2r-V5-attB3

Plasmid

#162909

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal V5 epitope tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

V5 epitope tag

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-639 (attR4-attR2)

Plasmid

#161880

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Insect

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-624 (attR4-attR2)

Plasmid

#161879

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Insect

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pWDY1226

Plasmid

#138593

Purpose

Expresses NatL2-R156A in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Expression

Bacterial

Mutation

changed Arginine 156 to Alanine

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pWDY1222

Plasmid

#138592

Purpose

Expresses NatL2-K418A in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Expression

Bacterial

Mutation

changed Lysine 418 to Alanine

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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IgH intron enhancer

Plasmid

#127247

Purpose

To maximize gene expression in B cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

heavy chain enhancer

Expression

Mammalian

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pTD3685

Plasmid

#113391

Purpose

alpha factor inducible Ty3

Depositor

Suzanne Sandmeyer

Article

Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

Insert

Ty3

Expression

Yeast

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pLex-FLAG-FASTKD4 mutant delta 226

Plasmid

#73621

Purpose

Expression vector containing FASTKD4 mutant delta 226

Depositor

Vamsi Mootha

Article

Wolf et al Cell Rep. 2014 May 8;7(3):918-31. doi: 1

Insert

FASTKD4 (TBRG4 Human)

Mutation

Y616A, L617A, K618A, K620A

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAS2-1-Brr2

Plasmid

#99995

Purpose

Express yeast Brr2 with Y2H binding domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Brr2 (BRR2 Budding Yeast)

Tags

Y2H binding domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pACT2-Rcl1

Plasmid

#99992

Purpose

Express yeast Rcl1 with Y2H activation domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Rcl1 (RCL1 Budding Yeast)

Tags

Y2H activation domain

Expression

Yeast

Mutation

Contains amino acids 1-217 of Rcl1

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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