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PB CAG-L-bpA-2xSV40pA-L-tdGFP-Luciferase_shRNAmir

Plasmid

#178045

Purpose

CAG tdGFP Luciferase shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

Depositor

Mario Capecchi

Insert

Luciferase_1309 shRNAmir

Use

Cre/Lox and RNAi; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA

Plasmid

#37090

Purpose

Expresses humanized ChR2(H134R)-mCherry in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

hChr2(H134R)-mCherry

Use

AAV and Cre/Lox; Cre-off

Tags

mCherry

Expression

Mutation

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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EC71167

Plasmid

#154066

Purpose

Level 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFP

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

ZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct

Use

Synthetic Biology

Tags

Expression

Mutation

All BsaI, Esp3I and BPiI restriction sites were r…

Promoter

Zea mays Ubiquitin Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pJH4143

Plasmid

#191531

Purpose

Punc-17-Cre unc-54 3'UTR C.elegans for Cre/Lox

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Cre

Use

Cre/Lox

Tags

no taq

Expression

Mutation

Promoter

Punc-17

Available since

Dec. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_3

Plasmid

#178049

Purpose

CAG tdGFP Lrig1 shRNAmir-3 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

Depositor

Mario Capecchi

Insert

Lrig1 shRNAmir

Use

Cre/Lox and RNAi; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_2

Plasmid

#178048

Purpose

CAG tdGFP Lrig1 shRNAmir-2 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

Depositor

Mario Capecchi

Insert

Lrig1 shRNAmir

Use

Cre/Lox and RNAi; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_1

Plasmid

#178047

Purpose

CAG tdGFP Lrig1 shRNAmir-1 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

Depositor

Mario Capecchi

Insert

Lrig1 shRNAmir

Use

Cre/Lox and RNAi; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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H2B-sfGFP-T2A-iCre

Plasmid

#178546

Purpose

pCMV-Histone H2B-sfGFP-T2A-iCre-bpA-FRT-Neo*-FRT

Depositor

Mario Capecchi

Insert

Histone H2B-sfGFP-T2A-iCre

Use

Cre/Lox and Mouse Targeting; Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGY577

Plasmid

#166663

Purpose

Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Cre: E340A, D341A

Promoter

CMV

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJH4237

Plasmid

#191538

Purpose

Ptwk-40s::Cre unc-54 3' UTR C.elegans for Cre/Lox

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Cre

Use

Cre/Lox

Tags

no taq

Expression

Worm

Mutation

Promoter

Ptwk-40

Available since

Nov. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO.DEST.puro

Plasmid

#32682

Purpose

Lentiviral cDNA/shRNA gateway destination vectors

Depositor

Ming-Sound Tsao

Article

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.DEST.EGFP

Plasmid

#32684

Purpose

Lentiviral cDNA/shRNA gateway destination vectors

Depositor

Ming-Sound Tsao

Article

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR.CMV

Plasmid

#32688

Purpose

Mammalian expression and gateway entry vector

Depositor

Ming-Sound Tsao

Article

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.DEST.hygro

Plasmid

#32685

Purpose

Lentiviral cDNA/shRNA gateway destination vectors

Depositor

Ming-Sound Tsao

Article

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.DEST.YFP

Plasmid

#32683

Purpose

Lentiviral cDNA/shRNA gateway destination vectors

Depositor

Ming-Sound Tsao

Article

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pRho-Cre

Plasmid

#13779

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Rhodopsin promoter-Cre

Use

Cre/Lox

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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PB CAG-F-intron-L-F-bpA-SV40pA-L-td-sfGFP

Plasmid

#178433

Purpose

CAG tdGFP FLP/FRT cre/lox "NOT" reporter piggyBac transposon (bpA SV40pA stop)

Depositor

Mario Capecchi

Insert

td-sfGFP

Use

Cre/Lox; Flp/frt, piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSico p53

Plasmid

#12089

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Insert

p53 shRNA (Trp53 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

EGFP is expressed from this plasmid as a marker, …

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pSicoR p53

Plasmid

#12090

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Insert

p53 shRNA (Trp53 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

EGFP is expressed from this plasmid as a marker, …

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pSicoR Dnmt1

Plasmid

#12166

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Insert

Dnmt1 shRNA (Dnmt1 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

EGFP is expressed from this plasmid as a marker, …

Promoter

Available since

June 30, 2006

Availability

Academic Institutions and Nonprofits only