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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,414 results for: puc57

Showing: 561 - 580 of 1414 results

  7. G555

    Plasmid
    #216483
    Purpose
    Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.
    Depositor
    Christophe Danelon
    Insert
    plsB, plsC, cdsA, pssA
    Use
    Cell-free expression
    Tags
    Expression
    Mutation
    Promoter
    T7 for each gene
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    216483-plasmid-map-sequence-id-430343

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  8. AICSDP-57: SMC1A-mEGFP

    Plasmid
    #114406
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SMC1A
    Depositor
    Allen Institute for Cell Science
    Insert
    SMC1A Homology Arms with linker-mEGFP (SMC1A Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114406-plasmid-map-sequence-id-220833

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  9. AICSDP-117:POLR2A-mEGFP

    Plasmid
    #164500
    Purpose
    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A
    Depositor
    Allen Institute for Cell Science
    Insert
    POLR2A Homology Arms with mEGFP-linker (POLR2A Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Feb. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    164500-plasmid-map-sequence-id-324900

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  11. AICSDP-69: NPM1-mTagRFP-T

    Plasmid
    #124608
    Purpose
    Homology arms and linker-mTagRFP-T sequence for C-terminus tagging of human NPM1
    Depositor
    Allen Institute for Cell Science
    Insert
    NPM1 Homology Arms with linker-mTagRFP-T (NPM1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    124608-plasmid-map-sequence-id-244304

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  12. MTST5

    Plasmid
    #86602
    Purpose
    Custom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.
    Depositor
    Mary Ann Moran
    Article
    Satinsky et al Methods Enzymol. 2013;531:237-50. doi: 1
    Insert
    MTST5
    Use
    Internal standard reverse transcription
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Sept. 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    86602-plasmid-map-sequence-id-193516

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  16. MTST6

    Plasmid
    #86603
    Purpose
    Custom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.
    Depositor
    Mary Ann Moran
    Article
    Satinsky et al Methods Enzymol. 2013;531:237-50. doi: 1
    Insert
    MTST6
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    86603-plasmid-map-sequence-id-193515

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  17. pZH516

    Plasmid
    #102669
    Purpose
    Constitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102669-plasmid-map-sequence-id-199392

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  18. pZH514

    Plasmid
    #102667
    Purpose
    Constitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    102667-plasmid-map-sequence-id-199386

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  20. AICSDP-144:CTCF-mEGFP

    Plasmid
    #168799
    Purpose
    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human CTCF
    Depositor
    Allen Institute for Cell Science
    Insert
    CTCF (CTCF Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    June 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    168799-plasmid-map-sequence-id-334694

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Showing: 561 - 580 of 1414 results