Addgene

We narrowed to 17,327 results for: MUT

Showing: 841 - 870 of 17327 results

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pLR5-CAG-44B12mut23(Ser5ph)-SNAP

Plasmid

#174877

Purpose

PiggyBac transposon vector for expression of RNAPII Ser5ph mintbody

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

RNAPII Ser5ph mintbody-SNAP

Use

Piggybac vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX

Plasmid

#164612

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in mammalian cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Use

Tags

Expression

Mammalian

Mutation

S383T, F177A

Promoter

CMV

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0234 pCMV - huDisCas7-11 dead mutant

Plasmid

#172509

Purpose

Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 dead mutant

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Zeo-gfpmut3

Plasmid

#65037

Purpose

ZeoR mini-Tn7 vector, mobilizable, for GFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

gfpmut3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pDae069 (eMutaT7[PmCDA1] with optimized RBS)

Plasmid

#187621

Purpose

Expresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Insert

PmCDA1-T7RNAP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX

Plasmid

#167583

Purpose

Express the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cells

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag FKHR AAA mutant

Plasmid

#13508

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR AAA (FOXO1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

T24A S256A S319A

Promoter

Available since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pGL4 mIL-17 2kb RORgamma-t mut

Plasmid

#20127

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Tags

Expression

Mutation

RORgamma-t binding site in 0.9kb of 2kb promoter …

Promoter

Available since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only
pRK5-mEGFP-HMGB1-MUT-IDR2

Plasmid

#194552

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) IDR Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-MUT-Patchless

Plasmid

#194553

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "Patchless" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44, truncation by stop codon after Lys209

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-2xPH-TAPP1 R211L mutant

Plasmid

#161991

Purpose

Expresses two mutated tandem repeats of Tapp1 PH domain (phosphoinositide binding-deficient mutant of TAPP1, R211L, can't bind PI(3,4)P2). Fused to eGFP

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PH-TAPP1 (PLEKHA1 Human)

Use

Tags

Expression

Mammalian

Mutation

R211L

Promoter

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pDsRed Monomer C1-YAP2-S127A mutant

Plasmid

#19058

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

Red-FPP

Expression

Mammalian

Mutation

Serine 127 changed to Alanine

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pSG5-Crkl mut SH2/SH3

Plasmid

#41704

Depositor

Nora Heisterkamp

Insert

Crkl (CRKL Human)

Use

Tags

Expression

Mammalian

Mutation

R39K and W160K

Promoter

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
mCherry-p62-LIR-mutant (LIRAAAA)

Plasmid

#187981

Purpose

Plasmid for the expression of a p62 mutant (LIR mutant: p62 3xDW + silent mutation). Internal reference: SMC528

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

DDDW335-338AAAA (LIR mutant), silent mutation siR…

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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p2xFlag CMV2-YAP2-S127A mutant

Plasmid

#19050

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Serine 127 changed to Alanine

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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p2xFlag CMV2-YAP2-S127D mutant

Plasmid

#19051

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Serine 127 changed to Aspartic Acid

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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PB-EF1-IRES-NEO mutant ITR

Plasmid

#67905

Purpose

PiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutation

Depositor

Alex Kentsis

Article

Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

Insert

NeoR (neoR Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV5 TBRI-HA with various GS domain mutations

Plasmid

#19162

Depositor

Joan Massague

Insert

transforming growth factor, beta receptor 1 (TGFBR1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

T204D (GS domain mutation)

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

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pPB-TetON-mEGFP-HMGB1-MUT

Plasmid

#194562

Purpose

Dox-inducible Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44

Promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Siah1 Ring Mutant

Plasmid

#122040

Purpose

express Siah1 RM

Depositor

Ze'ev Ronai

Insert

Siah1 RM

Use

Tags

Flag

Expression

Mammalian

Mutation

Ring mutant H58A and C62A

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFPC1/GgVcl 1-1066 T12 mutant

Plasmid

#46266

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

Insert

Vcl T12 mutant (VCL Chicken)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T12 mutant (D974;K975;R976; R978A) autoinhibition…

Promoter

CMV

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS5-YFP-NLS-mut

Plasmid

#120832

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

ZIKV NS5-eYFP "NLS-mut"

Use

Tags

eYFP-V5

Expression

Mammalian

Mutation

R372A/Q373A/K387A/H388A/K389A

Promoter

CMV

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin

Plasmid

#199585

Purpose

Express tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vector

Depositor

Michael Lin

Article

Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

Inserts

ERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
AkaLuc
Blasticidin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pVQ CMV NanoV1Mutant-2a-EGFP ferritin

Plasmid

#79650

Purpose

Expresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin chimera fusion protein

Depositor

Jeffrey Friedman

Article

Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

Inserts

transient receptor potential vanilloid 1 (Trpv1 Rat)
camelid anti-GFP nanobody
Enhanced green fluorescent protein
mouse ferritin light chain (Ftl1 Mouse)
mouse ferritin heavy chain (Fth1 Mouse)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

isoleucine 679 changed to lysine

Promoter

CMV

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV/myc/mito-mutant-hogg1

Plasmid

#18708

Depositor

David Sidransky

Article

Chatterjee et al BMC Cancer. 2006 . 6():235.

Insert

8-oxoguanine DNA glycosylase (OGG1 Human)

Use

Tags

Expression

Mammalian

Mutation

Amino acid 229, Arginine to Glutamine

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
p182 pK7-HDAC1 mut (GFP)

Plasmid

#11055

Depositor

Ramesh Shivdasani

Article

Tou et al Mol Cell Biol. 2004 Apr . 24(8):3132-9.

Insert

HDAC1 (Hdac1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Deletion of aa 1-56: deacetylase domain deletion

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_UBA/UIM/ UBM-mut

Plasmid

#187154

Purpose

mammalian cell expression of FLAG-HUWE1 L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

Promoter

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Siah2 Ring Mutant

Plasmid

#122042

Purpose

express Siah2 RM

Depositor

Ze'ev Ronai

Insert

Siah2 RM (Siah2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ring mutant deletion H98, L99A, V100G, C101A

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_g5-HT3.0mut

Plasmid

#208724

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al bioRxiv 2023.05.27.542566

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBI-GFP-Bub1-deletion mutant

Plasmid

#16626

Depositor

Bert Vogelstein

Article

Cahill et al Nature. 1998 Mar 19. 392(6673):300-3.

Insert

BUB1 deletion mutant (BUB1 Human)

Use

Tags

Expression

Mammalian

Mutation

Amino acids 1-75

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only