We narrowed to 1,005 results for: Psp

1. EZH2-Promoter-gRNA

   Plasmid

   #131329

   Purpose

   gRNA to generate endogenously tagged -tetR-EZH2

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EZH2-Promoter-gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EED-F97A

   Plasmid

   #131326

   Purpose

   gRNA to generate F97A cage-mutant EED

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EED-KO-gRNA-2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EED-KO-gRNA-1

   Plasmid

   #131321

   Purpose

   gRNA to knockout EED. Use with EED-KO-gRNA-2

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EED-KO-gRNA-1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

4. pTRE/SP M87 N184X

   Plasmid

   #92373

   Purpose

   Inducible expression of M87 N184X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1-460 bp deleted, N184X

   Promoter

   Tet-responsive P tight

   Available since

   June 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTRE/SP S245X with 5'UTR

   Plasmid

   #92376

   Purpose

   Inducible expression of S245X spastin M1 and M87 in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S245X

   Promoter

   Tet-responsive P tight

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTRE/SP M87 S245X

   Plasmid

   #92374

   Purpose

   Inducible expression of M87 S245X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1-460 bp deleted, S245X

   Promoter

   Tet-responsive P tight

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTRE/SP M1 S245X

   Plasmid

   #92372

   Purpose

   Inducible expression of M1S245X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1-194 bp of 5'UTR deleted, S245X

   Promoter

   Tet-responsive P tight

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTRE/SP M1 N184X

   Plasmid

   #92371

   Purpose

   Inducible expression of M1 N184X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1-194 bp of 5'UTR deleted, N184X

   Promoter

   Tet-responsive P tight

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-Tag/SP S245X with 5'UTR

   Plasmid

   #92370

   Purpose

   expression of 245X spastin M1 and M87 in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S245X

   Promoter

   CMV

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-Tag/M87 N184X

    Plasmid

    #92367

    Purpose

    expression of M87 N184X spastin in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

    Insert

    spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1-460 bp deleted, N184X

    Promoter

    CMV

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-Tag/M1 N184X

    Plasmid

    #92365

    Purpose

    expression of M1 N184X spastin in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

    Insert

    spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1-194 bp of 5'UTR deleted, N184X

    Promoter

    CMV

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-Tag 3B/myc-M87L195V

    Plasmid

    #87721

    Purpose

    Expresses myc-M87L195V spastin isoform in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    M87 isoform (deletion of nt 1-490 - 5'UTR an…

    Promoter

    CMV

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-Tag/S46X without 5'UTR

    Plasmid

    #87730

    Purpose

    Expresses S46X spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S46X; 5'UTR deleted (1-190 bp)

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-Tag/S44L without 5'UTR

    Plasmid

    #87728

    Purpose

    Expresses S44L spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S44L; 5'UTR deleted (1-190 bp)

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-Tag/L195V without 5'UTR

    Plasmid

    #87726

    Purpose

    Expresses L195V spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L195V; 5'UTR deleted (1-190 bp)

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-Tag/E112K without 5'UTR

    Plasmid

    #87724

    Purpose

    Expresses E112K spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E112K; 5'UTR deleted (1-190 bp)

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-Tag 3B/myc-M87E112K

    Plasmid

    #87720

    Purpose

    Expresses myc-M87E112K spastin isoform in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    M87 isoform (deletion of nt 1-490 - 5'UTR an…

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TRE-VEGFA EF1a-rtTA3

    Plasmid

    #195881

    Purpose

    Lentiviral construct of doxycycline inducible VEGFA

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    VEGFA (Vegfa Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDG458

    Plasmid

    #100900

    Purpose

    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX458-Ef1a-Cas9-H2B-mCherry

    Plasmid

    #159655

    Purpose

    Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMGS7 (AAVS1sgRNA)

    Plasmid

    #126582

    Purpose

    A CRISPR/Cas9 construct to target the AAVS1 locus in humans

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    AAVS1 sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mApple-6xHp

    Plasmid

    #214410

    Purpose

    Lipid droplet localization of 6xHp of Spastin fused to mApple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    Spastin aa 43-92 (SPAST Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX458-Ef1a-Cas9-H2B-GFP

    Plasmid

    #159654

    Purpose

    Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. hAAVS1-gs4_EF1a-Cas9-2A-GFP

    Plasmid

    #118414

    Purpose

    CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    gRNA AAVS1 (AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCPH2A.X-GS3

    Plasmid

    #204745

    Purpose

    Expression of Cas9 and human H2AX

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. U6GRNA

    Plasmid

    #68370

    Purpose

    gRNA scaffold with hU6 promoter

    Depositor

    Eric-Paul Bennett

    Article

    Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mApple-M1 Spastin

    Plasmid

    #134461

    Purpose

    M1 Spastin expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Met to Ala change at amino acid 87

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. px459-TDP-43 sgRNA1

    Plasmid

    #133762

    Purpose

    Expresses Cas9 and human TDP-43 sgRNA

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPBC-G6s

    Plasmid

    #62812

    Purpose

    pPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-GCaMP6s-ITR
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-SpCas9-2A-GFP-noITR

    Plasmid

    #118415

    Purpose

    Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge