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pCMV_SEPT9_i1 NCmut2-msfGFP

Plasmid

#180330

Purpose

mammalian expression of human SEPT9_i1 NCmut2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 I281D, M288D

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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STK16 gRNA (BRDN0001146155)

Plasmid

#75933

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK16

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK16 (STK16 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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STK16 gRNA (BRDN0001146199)

Plasmid

#75934

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK16

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK16 (STK16 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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STK16 gRNA (BRDN0001145107)

Plasmid

#75935

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK16

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK16 (STK16 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pORTMAGE311B

Plasmid

#120418

Purpose

Expresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanR

Depositor

Csaba Pál

Article

Szili et al bioRxiv (2018)

Inserts

MutL E32K
Lambda Beta
XylS

Use

Tags

Expression

Bacterial

Mutation

E32K mutation conferring dominant mutator phenoty…

Promoter

Pm

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

Plasmid

#176836

Purpose

Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP

Use

Unspecified

Tags

Expression

Mutation

See Depositor Comments Below

Promoter

Tet operator (tetracycline inducible expression)

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-GAL4-IL35-IRES-mCherry-PGK-BFP

Plasmid

#223210

Purpose

Lentiviral vector - mCherry reporter and IL35 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

Depositor

Wendell Lim

Article

Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

Insert

IL35 (IL12A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMCSF-R(DD)-luc

Plasmid

#12424

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1994 Dec . 14(12):8085-95

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Tags

Expression

Mutation

Deletion from bp-86 to -37 (created a KpnI site i…

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pMCSF-R(mB)-luc

Plasmid

#12422

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Tags

Expression

Mutation

AML1 binding site mutation (created a KpnI site i…

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
Anti-WAVE1/SCAR [K91/36R]

Plasmid

#128629

Purpose

Mammalian Expression Plasmid of anti-WAVE1/SCAR (Human). Derived from hybridoma K91/36.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-WAVE1/SCAR (Human) recombinant mouse monoclonal antibody (WASF1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept

Plasmid

#182424

Purpose

Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteins

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-LZ-mNeongreen-Etanercept (TNFRSF1B Human)
VNp-LZ

Use

Tags

Vesicle Nucleating peptide (VNp), and Vesicle Nuc…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMCSF-R(mA)-luc

Plasmid

#12421

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Tags

Expression

Mutation

C/EBP binding site mutation (created a KpnI site …

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pBMN-neo-Flag-TNFR1 L380A Del229-244

Plasmid

#44098

Depositor

Martin Kluger

Article

D'Alessio et al J Biol Chem. 2010 Jul 30;285(31):23868-7

Insert

human tumor necrosis factor receptor 1 (TNFRSF1A Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

L380A

Promoter

Available since

April 8, 2013

Availability

Academic Institutions and Nonprofits only
pInducer20 2xHA GPR137

Plasmid

#226813

Purpose

Tet-inducible lentiviral vector for 2xHA GPR137 expression

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR137 (GPR137 Human)

Use

Lentiviral

Tags

2xHA tag followed by a GDPPVAT linker

Expression

Mutation

Sequence optimized from NP_001164351

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-RELT-COMP5AP-AviTag-9xHis

Plasmid

#157093

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

RELT (RELT Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pnCS_SEPT7_SEPT9_i1-i5-TEV-Strep

Plasmid

#180314

Purpose

bacterial co-expression of human SEPT7 and of human SEPT9_i1-i5

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 )

Use

Tags

TEV-Strep

Expression

Bacterial

Mutation

SEPT9_i1-i5

Promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S22A S23A-msfGFP

Plasmid

#180338

Purpose

mammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S22A S23A

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 R10A R15A-msfGFP

Plasmid

#180336

Purpose

mammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 R10A R15A

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S12A S13A-msfGFP

Plasmid

#180337

Purpose

mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S12A S13A

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pnCS_SEPT7_SEPT9_i1-TEV-Strep

Plasmid

#174500

Purpose

bacterial co-expression of human SEPT7 and of human SEPT9_i1

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 Human)

Use

Tags

TEV-Strep

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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