We narrowed to 2,461 results for: T2A

1. plenti-CMV-mCherry-T2A-GFP

   Plasmid

   #109427

   Purpose

   A lentiviral backbone expressing mCherry-T2A-eGFP off of a CMV promoter.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   mCherry T2A GFP
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Calb1-T2A-dgCre targeting vector

   Plasmid

   #114438

   Purpose

   Target dgCre into the endogenous mouse Calb1 locus at the stop codon

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   dgCre
   

   Use

   Mouse Targeting

   Tags

   DHFR domain and EGFP

   Promoter

   none; utilizes endogenous Calb1 promoter for expr…

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. PM-FRB-mRFP-T2A-FKBP-5-ptase

   Plasmid

   #40896

   Depositor

   Peter Varnai

   Article

   Toth et al J Cell Sci. 2012 May 1;125(Pt 9):2185-97

   Insert

   PM-FRB-mRFP-T2A-FKBP-5-ptase
   

   Available Since

   Jan. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Hpep1-betaArrestin2-T2A-mTagBFP2

   Plasmid

   #194680

   Purpose

   CMV driven co-expression of peptide complementing GPCR recorder (beta-arrestin 2 fusion) and mTagBFP2

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Hpep1-betaArrestin2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pELPS-eDHFR-YFP-T2A-Renilla

   Plasmid

   #193253

   Purpose

   Triple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase

   Depositor

   Mark Sellmyer

   Article

   Sellmyer et al Mol Ther. 2020 Jan 8;28(1):42-51. doi: 1

   Insert

   The eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
   

   Use

   Lentiviral

   Tags

   Renilla luciferase (rLuc) and yellow fluorescent …

   Mutation

   None

   Promoter

   EF1a

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-hSyn1-mRuby2-T2A-GCaMP6f

   Plasmid

   #197595

   Purpose

   Lentiviral transfer vector for the neuronal expression of mRuby2-T2A-GCaMP6f (fluorescent reporter for Calcium imaging)

   Depositor

   Patrik Verstreken

   Insert

   mRuby2-T2A-GCaMP6f
   

   Use

   Lentiviral

   Promoter

   hSynI

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti Vp64-dCas9 T2A mTagBFP2

   Plasmid

   #171052

   Purpose

   Expression of Vp64-dCas9 activation system

   Depositor

   Agnel Sfeir

   Article

   Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

   Insert

   Vp64-dCas9 T2A mTagBFP2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2

   Plasmid

   #167295

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   BAK (BAK1 Human)
   

   Expression

   Mammalian

   Available Since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUGW U6 gL1HSg2dCas9-KRAB-T2a-GFP

   Plasmid

   #234881

   Purpose

   L1HS-silencing plasmid (CRISPRi gRNA2)

   Depositor

   Johan Jakobsson

   Article

   Adami et al bioRxiv 2025.01.17.633315

   Insert

   LacZ gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUGW U6 gL1HSg1dCas9-KRAB-T2a-GFP

    Plasmid

    #234882

    Purpose

    L1HS-silencing plasmid (CRISPRi gRNA1)

    Depositor

    Johan Jakobsson

    Article

    Adami et al bioRxiv 2025.01.17.633315

    Insert

    L1HS-silencing plasmid (CRISPRi gRNA1)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRE-UBC-mCD24-T2A-GFP

    Plasmid

    #205448

    Purpose

    lentiviral plasmid for expression of mouse CD24

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    mCD24 (Cd24a Mouse)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFOXA2-T2A-mTagBFP2-PGK-Neo

    Plasmid

    #223191

    Purpose

    BFP reporter plasmid targeting the human FOXA2 gene

    Depositor

    Matthew Birket

    Article

    Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

    Insert

    FOXA2-T2A-mTagBFP2-PGK-Neo-FOXA2 (FOXA2 Human)
    

    Use

    Cre/Lox; Human targeting

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

    Plasmid

    #167296

    Purpose

    Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    BAX (BAX Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV Marina-T2A-nls-mCherry

    Plasmid

    #74216

    Purpose

    genetically-encoded fluorescent voltage indicator

    Depositor

    Vincent Pieribone

    Article

    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

    Insert

    fluorescent protein voltage sensor
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    CMV

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. 15x-QUAS-dTomato-T2A-GCaMP6s

    Plasmid

    #130666

    Purpose

    QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    15xQUAS-dTomato-T2A-GCaMP6s
    

    Use

    Ae. aegypti expression

    Expression

    Insect

    Promoter

    15xQUAS-TATA

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRE-UBC-mPD-L1-T2A-GFP

    Plasmid

    #205455

    Purpose

    lentiviral plasmid for expression of mouse PD-L1

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    mPD-L1 (Cd274 Mouse)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-KS-attB2-SA(2)-T2A-Gal4DBD-Hsp70

    Plasmid

    #62904

    Purpose

    Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4DBD-Hsp70
    

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP

    Plasmid

    #155307

    Purpose

    Rfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFP

    Depositor

    Stanley Qi

    Article

    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

    Insert

    Rfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6, EF1a

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRE-UBC-hCD59-T2A-GFP

    Plasmid

    #205460

    Purpose

    lentiviral plasmid for expression of human CD59

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD59 (CD59 Human)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-CB-IRES-copGFP-T2A-Puro

    Plasmid

    #72299

    Purpose

    Express gene of interest under CB promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    chicken beta-actin + CMV enhancer

    Available Since

    Jan. 25, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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