We narrowed to 2,116 results for: dcas9

1. pdCas9+sgRNA expression vector precursor

   Plasmid

   #171798

   Purpose

   PICASSO: Library expression vector precursor for paired dCas9+sgRNA expression

   Depositor

   Stephen Elledge

   Article

   Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO

   Available since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdCas9-DNMT3A-PuroR (ANV)_v2

   Plasmid

   #74408

   Purpose

   Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Synthetic, Human, S. pyogenes)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; E756A inactiva…

   Promoter

   CBh

   Available since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpdCas9-huTET1CD-T2A-GFP (PX458)

   Plasmid

   #129025

   Purpose

   targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA cloning site
   

   Use

   Tags

   HA-Tag, NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

   Plasmid

   #129026

   Purpose

   inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA cloning site
   

   Use

   Tags

   HA-Tag, NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGinB-8X(GGS)-dCas9-FLAG-NLS

   Plasmid

   #81205

   Purpose

   CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   pGinB-8X(GGS)-dCas9-FLAG-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast

   Plasmid

   #71216

   Purpose

   dCas9 fused to human DNMT3A catalytic domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-DNMT3A catalytic domain
   

   Use

   Lentiviral

   Tags

   dSpCas9-DNMT3A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-EF1a-SPdCas9-EGFP-2A-Blast

   Plasmid

   #71215

   Purpose

   dCas9 fused to EGFP

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-EGFP
   

   Use

   Lentiviral

   Tags

   dSpCas9-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti Vp64-dCas9 T2A mTagBFP2

   Plasmid

   #171052

   Purpose

   Expression of Vp64-dCas9 activation system

   Depositor

   Agnel Sfeir

   Article

   Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

   Insert

   Vp64-dCas9 T2A mTagBFP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-dCas9-MQ1(S317A)-EGFP

   Plasmid

   #89636

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR

   Tags

   3*FLAG and 6*His

   Expression

   Mammalian

   Mutation

   S317A, AGC-->GCC

   Promoter

   CMV

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)

    Plasmid

    #160623

    Purpose

    TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35S:dCas9:VPR-Tnos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid

    #171089

    Purpose

    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

    Inserts

    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3X FLAG and Nuclear Localization Signal

    Expression

    Mutation

    D10A, H840A

    Promoter

    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCCL-PGK-SPdCas9-BFP-DNMT3A

    Plasmid

    #66819

    Purpose

    dCas9 fused to BFP and the human DNMT3A catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3A, catalytic domain of DNMT3A
    

    Use

    Tags

    dSpCas9-BFP-DNMT3A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast

    Plasmid

    #71217

    Purpose

    dCas9 fused to human DNMT3B catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3B catalytic domain
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCXLE-dCas9VPH-T2A-GFP-shP53

    Plasmid

    #102895

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9VPH-T2A-GFP-shP53
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. SL2-sgTelo-MTSa/BFP/pdCas9-C1

    Plasmid

    #162760

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting telomeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgTelo-MTSa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCXLE-dCas9VPPH-T2A-GFP-shP53

    Plasmid

    #102897

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9VPPH-T2A-GFP-shP53
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. hSyn-GW-dCas9-SET2-IRES2-mCherry

    Plasmid

    #202089

    Purpose

    Neuron-specific expression vector of dCas9-Set2

    Depositor

    Elizabeth Heller

    Article

    Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

    Insert

    dCas9-Set2
    

    Use

    Tags

    3x FLAG and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-dCas9-MQ1(C141S)-EGFP

    Plasmid

    #89635

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG, 6*His, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    C141S, TGC-->TCC

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-dCas9-p300 Core (Y1467F)

    Plasmid

    #61362

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. SL2-sgTelo-MTSa/EGFP/pdCas9-C1

    Plasmid

    #162759

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting telomeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgTelo-MTSa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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