We narrowed to 1,093 results for: pcs2

1. pCS2+8-CYP2A6(C439A)-flag-tev-his

   Plasmid

   #227167

   Purpose

   For overexpression of CYP2A6 C439A mutant in mammalian cells and purification.

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   CYP2A6 (CYP2A6 Human)
   

   Use

   Tags

   Flag and His6

   Expression

   Mammalian

   Mutation

   changed cysteine 439 to alanine

   Promoter

   CMV and SP6

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCS2+8-aex-5-flag-tev-halo-his

   Plasmid

   #227152

   Purpose

   For T-REX experiments of aex-5.

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   aex-5 (aex-5 Nematode)
   

   Use

   Tags

   Flag, HaloTag, and His6

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SP6

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2+8-asp-2-flag-tev-halo-his

   Plasmid

   #227153

   Purpose

   For T-REX experiments of asp-2.

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   asp-2 (asp-2 Nematode)
   

   Use

   Tags

   Flag, HaloTag, and His6

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SP6

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCS2+8-CYP2A6-flag-tev-halo-his

   Plasmid

   #227154

   Purpose

   For T-REX experiments of CYP2A6. A reactive electrophilic species (RES) Sensor.

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   CYP2A6 (CYP2A6 Human)
   

   Use

   Tags

   Flag, HaloTag, and His6

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SP6

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCS2+8-PCSK1-flag-tev-halo-his

   Plasmid

   #227155

   Purpose

   For T-REX expriments of PCSK1.

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   PCSK1 (PCSK1 Human)
   

   Use

   Tags

   Flag, HaloTag, and His6

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SP6

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcs2-Δhel1/Δhel2HRI-GFP-IRES-mCherry

   Plasmid

   #226101

   Purpose

   HRI stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   HRI (EIF2AK1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   SIFI degron helices 1 & 2 deleted (Δ61-112)

   Promoter

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcs2-N-HRI(1-138)-GFP-IRES-mCherry

   Plasmid

   #226100

   Purpose

   HRI N-terminal region (residues 1-138) stability reporter construct for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   HRI (EIF2AK1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains only N-HRI (aa 1-138)

   Promoter

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCA31-pCS2-CMV-stdPCP-p65-HSF1

   Plasmid

   #199462

   Purpose

   Expression of stdPCP-PH in zebrafish embryos

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   stdPCP-PH
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCA30-pCS2-CMV-stdMCP-p65-HSF1

   Plasmid

   #199461

   Purpose

   Expression of stdMCP-PH in zebrafish embryos

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   stdMCP-PH
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCA29-pCS2-CMV-Zebrafish NarTag (eGFP)

    Plasmid

    #199460

    Purpose

    Expression of Zebrafish NarTag (eGFP harbors 9XMS2 in its intron) in zebrafish embryos

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    Zebrafish NarTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2 Halo-Tev- HSPB7 (zebrafish) C117S

    Plasmid

    #108915

    Purpose

    Express C117S mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cells

    Depositor

    Yimon Aye

    Article

    Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

    Insert

    Halo-TEV-HSPB7
    

    Use

    Tags

    Halo-TEV

    Expression

    Mammalian

    Mutation

    Cysteine 117 to Serine

    Promoter

    SP6

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2 Halo-Tev- HSPB7(zebrafish) C49S

    Plasmid

    #108914

    Purpose

    Express C49S mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cells

    Depositor

    Yimon Aye

    Article

    Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

    Insert

    Halo-TEV-HSPB7
    

    Use

    Tags

    Halo-TEV

    Expression

    Mammalian

    Mutation

    Cysteine 49 to Serine

    Promoter

    SP6

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124431

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafish

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Use

    Zebrafish (if expression level is too high in zeb…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2plus-mCerulean 156-239-GGGS-TDP-43 M337V

    Plasmid

    #162618

    Purpose

    Allows for transcription of mCerulean fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M337V

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCS2plus-mVenus1-155-I152L-GGGS-TDP43 M337V

    Plasmid

    #162615

    Purpose

    Allows for transcription of improved mVenus I152L fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M337V

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCS2 Halo-Tev- HSPB7 (zebrafish) C49S and C117S

    Plasmid

    #108913

    Purpose

    Express C49S/C117S double mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cells

    Depositor

    Yimon Aye

    Article

    Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

    Insert

    Halo-TEV-HSPB7
    

    Use

    Tags

    Halo-TEV

    Expression

    Mammalian

    Mutation

    Cysteine 49 to Serine, Cysteine 117 to Serine

    Promoter

    SP6

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS2+8-CYP2A6(C14A)-flag-tev-halo-his

    Plasmid

    #227159

    Purpose

    For T-REX experiments of CYP2A6 C14A mutant. A reactive electrophilic species (RES) Sensor.

    Depositor

    Yimon Aye

    Article

    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

    Insert

    CYP2A6 (CYP2A6 Human)
    

    Use

    Tags

    Flag, HaloTag, and His6

    Expression

    Mammalian

    Mutation

    changed cysteine 14 to alanine

    Promoter

    CMV and SP6

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2+8-CYP2A6(C82A)-flag-tev-halo-his

    Plasmid

    #227160

    Purpose

    For T-REX experiments of CYP2A6 C82A mutant. Less sensing ability to reactive electrophilic species (RES).

    Depositor

    Yimon Aye

    Article

    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

    Insert

    CYP2A6 (CYP2A6 Human)
    

    Use

    Tags

    Flag, HaloTag, and His6

    Expression

    Mammalian

    Mutation

    changed cysteine 82 to alanine

    Promoter

    CMV and SP6

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCS2+8-CYP2A6(C439A)-flag-tev-halo-his

    Plasmid

    #227161

    Purpose

    For T-REX experiments of CYP2A6 C439A mutant. Less sensing ability to reactive electrophilic species (RES).

    Depositor

    Yimon Aye

    Article

    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

    Insert

    CYP2A6 (CYP2A6 Human)
    

    Use

    Tags

    Flag, HaloTag, and His6

    Expression

    Mammalian

    Mutation

    changed cysteine 439 to alanine

    Promoter

    CMV and SP6

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2+8-cyp-33e1-flag-tev-halo-his

    Plasmid

    #227151

    Purpose

    For T-REX experiments of cyp-33e1. A reactive electrophilic species (RES) Sensor.

    Depositor

    Yimon Aye

    Article

    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

    Insert

    cyp-33e1 (cyp-33E1 Nematode)
    

    Use

    Tags

    Flag, HaloTag, and His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SP6

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge