We narrowed to 6,027 results for: ATS

1. TL1 alphaDdCBE 1-2 (right, DddA11-N)

   Plasmid

   #222000

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-1/2 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A | TALE: S1330I, A1341V,…

   Promoter

   CMV

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TL1 alphaDdCBE 2 (left, DddA11-C)

   Plasmid

   #222001

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-2 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A | DddA: T1413I

   Promoter

   CMV

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TL1 alphaDdCBE 3_CN (left, DddA11-C)

   Plasmid

   #222002

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_CN left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A | DddA: T1413I

   Promoter

   CMV

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #210736

   Purpose

   Coding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeats

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   SaSp D10A Cas9
   Merry sgCAG
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

   Promoter

   H1 and eCMV

   Available since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #210735

   Purpose

   Coding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeats

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   SaSp D10A Cas9
   Gollum sgCAG
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   D10A, N terminal Sa Cas9, C-terminal Sp Cas9

   Promoter

   H1 and eCMV

   Available since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT2/sh-FYN1-GFP_Seq_C

   Plasmid

   #201399

   Purpose

   Knockdown of FYN tyrosine kinase. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Comba et al Neuro Oncol. 2020 Jun 9;22(6):806-818. d

   Insert

   FYN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. 4LNDD-pCDNA3.1(+)

   Plasmid

   #172227

   Purpose

   Expresses human ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) in mammalian cells

   Depositor

   Joshua Rosenthal

   Article

   Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

   Insert

   Lambda N peptide - human ADAR2 Deaminase Domain
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLz26_CsoS2_N1-N2_GFP

   Plasmid

   #140851

   Purpose

   His-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 NTD repeats 1-2 fused to GFP
   

   Use

   Tags

   Hexahistidine tag, Strep-Tag II, and Superfolder …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-X2DM

   Plasmid

   #115571

   Purpose

   GFP-X2 where both of the consensus motif's lysines are mutated to arginine

   Depositor

   Michael Downey

   Article

   Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

   Insert

   Green Fluorescent Protein
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Two repeats of the Gcn5 consensus motif are tagge…

   Promoter

   ADH1

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

10. GFP-X2R2

    Plasmid

    #115570

    Purpose

    GFP-X2 where the second consensus motif's lysine is mutated to arginine

    Depositor

    Michael Downey

    Article

    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

    Insert

    Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Two repeats of the Gcn5 consensus motif are tagge…

    Promoter

    ADH1

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-X2R1

    Plasmid

    #115569

    Purpose

    GFP-X2 where the first consensus motif's lysine is mutated to arginine

    Depositor

    Michael Downey

    Article

    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

    Insert

    Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Two repeats of the Gcn5 consensus motif are tagge…

    Promoter

    ADH1

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3/F-tractin=mStayGold

    Plasmid

    #212019

    Purpose

    For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    F-tractin
    

    Use

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3/mStayGold(c4)=UtrCH

    Plasmid

    #212020

    Purpose

    For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    UtrCH
    

    Use

    Tags

    mStayGold(c4)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. eGFP-KIF1A

    Plasmid

    #172206

    Purpose

    Expresses KIF1A tagged with eGFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

    Insert

    KIF1A (Kif1a Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSET-6xTR-TUBE

    Plasmid

    #110313

    Purpose

    E. coli expression and purification of 6xTR-TUBE

    Depositor

    Yasushi Saeki

    Article

    Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

    Insert

    6xTR-TUBE (UBQLN1 Human)
    

    Use

    Tags

    N-terminal His6-T7 tag

    Expression

    Bacterial

    Mutation

    All Arg residues in the UBA domain are mutated to…

    Promoter

    T7

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL4-UPRE-luc2P-Hygro

    Plasmid

    #101788

    Purpose

    Luciferase reporter plasmid containing three tandem repeats of the unfolded protein response element (UPRE)

    Depositor

    Seiichi Oyadomari

    Article

    Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

    Insert

    3xUPRE
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    minimal TATA-box promoter with low basal activity

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL4-ERSE1-luc2P-Hygro

    Plasmid

    #101789

    Purpose

    Luferase reporter plasmid containing three tandem repeats of the ER stress response element-I (ERSE-I)

    Depositor

    Seiichi Oyadomari

    Article

    Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

    Insert

    3xERSE-I
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    minimal TATA-box promoter with low basal activity

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX SH3(2)-4R

    Plasmid

    #112090

    Purpose

    Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3(2)-4R (NCK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX SH3(2)-3R

    Plasmid

    #112091

    Purpose

    Bacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3(2)-3R (NCK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)

    Plasmid

    #193048

    Purpose

    Heatshock expressed Cas9

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    hsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    hsp16.41p

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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