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V207 pRETRO-Triple-Flag SD - Acceptor

Plasmid

#11709

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox and Retroviral; Acceptor vector

Tags

3xFLAG

Expression

Mammalian

Available Since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pCY 3050-07

Plasmid

#36223

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

Streptoalloteichus hindustanus bleomycin (Sh ble)

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-Venus-Zeocin

Available Since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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mg182

Plasmid

#34662

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A2;B2;C1) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pCMB-PLAr

Plasmid

#61201

Purpose

Fluorescent reporter for plastids MtRubisco1sp-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRubisco1sp-mCherry

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pME-mir126new

Plasmid

#67712

Purpose

Replacement for 26033 776 pME-mir-126

Depositor

Nathan Lawson

Article

Nicoli et al Nature. 2010 Apr 22. 464(7292):1196-200.

Insert

mir126a (mir126a Zebrafish)

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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V677 pDNR MCS BE lacZ alpha - Donor

Plasmid

#11694

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Donor vector

Available Since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pMD19T-slr0230-PL31-sgRNANT1-KmR

Plasmid

#73221

Purpose

Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli

Depositor

Paul Hudson

Article

Yao et al ACS Synth Biol. 2015 Dec 28.

Insert

PL31-sgRNA NT1

Expression

Bacterial

Promoter

PL31

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cyEGFP-spo5DSR

Plasmid

#49867

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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mg172

Plasmid

#34652

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A1;B1;C0) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pERB172

Plasmid

#58290

Purpose

const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^485-718-AAA

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-GFP-FKBPx3
inducible FRB-mCh
FKBP-targeted miRNA
FRB-mCh-Mad1^485-718-AAA

Tags

GFP and mCherry

Expression

Mammalian

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pERB171

Plasmid

#58289

Purpose

const. Mis12-FKBP3; ind. FRB-mCH-Mad1^485-718

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-FKBPx3
inducible FRB-mCh-Mad1^485-718
FKBP-targeted miRNA

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pET12a-AL-09 FL C214S

Plasmid

#47086

Depositor

Marina Ramirez-Alvarado

Article

Levinson et al Sci Rep. 2013;3:1278. doi: 10.1038/srep0

Insert

AL-09 FL C214S (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

C214S

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
Cenp-B INCENP Δcen mCherry

Plasmid

#45227

Depositor

Michael Lampson, Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

vsv Cenp-B INCENP Δcen mCherry

Mutation

CEN box deleted from INCENP (aa1-46)

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
V954 - pDNR MCS SA no SA CmR - Donor

Plasmid

#11697

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Donor vector

Available Since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pCambia2300-Aar1-ccdB-GmCH

Plasmid

#254405

Purpose

Gateway-compatible destination vector for bifluorescence complementation (BiFC). The plasmid carries Gus-mCherry expressed from the Arabidopsis Ubiquitin 10 promoter.

Depositor

Maria Harrison

Article

Ivanov et al Science. 2024 Jan 26;383(6681):443-448.

Type

Empty backbone

Expression

Plant

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pFY20

Plasmid

#253057

Purpose

low copy episomal cloning vector pFY20 for use in fission yeast

Depositor

Mari Davidson, Wayne Wahls

Article

Wahls et al Yeast. 2008 Sep;25(9):643-50. doi: 10.10

Type

Empty backbone

Use

Low copy number, episomal cloning vector

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pSpyB-Tn7sg

Plasmid

#248694

Purpose

sgRNA expression vector - pBG35 promoter with Tn7sg negative control sgRNA

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Tn7sg

Expression

Bacterial

Promoter

BG35

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pNA-Z

Plasmid

#238995

Purpose

Node A carrying sgRNA-2 that can be used for the assembly of pEND-Z11 or pEND-ZY

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

sgRNA-2

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pJW53(uTEV3-DHFR2.0-IRES-mCherry)

Plasmid

#248363

Purpose

Lentiviral vector that can express destabilized uTEV3. Expressed off of CMV promoter with mCherry marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

uTEV3-DHFR2.0

Use

Lentiviral

Promoter

CMV

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-11Y

Plasmid

#238999

Purpose

Final circuit with -Ara: OFF, +Ara: OFF behaviour

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

Circuit with no sfGFP expression (-Ara: OFF, +Ara: OFF)

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEND-ZY

Plasmid

#239002

Purpose

Final circuit with -Ara: OFF, +Ara: ON behaviour

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

Circuit with sfGFP expression only in the presence of Ara (-Ara: OFF, +Ara: ON)

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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CAG-iRFP

Plasmid

#140460

Purpose

Encodes iRFP driven by the CAG promoter

Depositor

Mark Emerson

Article

Buenaventura et al Dev Biol. 2018 Nov 1;443(1):35-49. doi:

Inserts

CAG promoter
iRFP670

Use

Chicken expression

Expression

Mammalian

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pVSr

Plasmid

#234930

Purpose

Virus-induced gene silencing (VIGS) reporter vector

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Type

Empty backbone

Expression

Plant

Available Since

Sept. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS07

Plasmid

#230070

Purpose

Inducible Promoter (rhamnose-induced, BT3763/rhaR system), replicates via RepA; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Article

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Bacterial

Promoter

rhamnose-inducible (BT3763)

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMS08

Plasmid

#230072

Purpose

Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; N-/C-terminal mScarlet-I fusion, ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Article

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG and mScarlet-I

Expression

Bacterial

Promoter

RpoD (bt_1311/BT_RS06635)

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pNA-Y

Plasmid

#238993

Purpose

Node A carrying sgRNA-1 that can be used for the assembly of pEND-Y11

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

sgRNA-1

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSIn-H3K9ac BS

Plasmid

#235131

Purpose

H3K9ac FRET biosensor for real-time, single-cell monitoring of H3K9ac levels

Depositor

Qin Peng

Article

Li et al Med-X. 2024; 2, 26. doi: 10.1007/s44258-

Insert

BD1-Ypet-34mer-ECFP-H3

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pSIn-H3K9ac Y97F BS

Plasmid

#235132

Purpose

Mutant (negative control) Y97F biosensor for pSin-H3K9ac Full

Depositor

Qin Peng

Article

Li et al Med-X. 2024; 2, 26. doi: 10.1007/s44258-

Insert

BD1Y97F-Ypet-34mer-ECFP-H3

Use

Lentiviral

Expression

Mammalian

Mutation

BD1Y97F

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pccGFP-MalE-pos-2H01

Plasmid

#239760

Purpose

Positive control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of SEM5A

Tags

MBP and ToxR

Expression

Bacterial

Mutation

Changed Leucine 985 to Isoleucine

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEND-Z11

Plasmid

#239001

Purpose

Final circuit with -Ara: ON, +Ara: ON behaviour

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

Circuit with sfGFP expression (-Ara: ON, +Ara: ON)

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pIAR007

Plasmid

#217022

Purpose

deletion of aldB-II

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_2679 (pedH) and PP_2681 (pqqD-II)

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pIAR008

Plasmid

#217023

Purpose

deletion of amaB

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_5257 (amaA) and PP_5259

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pIAR009

Plasmid

#217024

Purpose

deletion of aldA

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_2486 (xenC) and PP_2488 (sad_I)

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pIAR010

Plasmid

#217025

Purpose

deletion of modABC

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_3827 (yrpB) and PP_3831

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pIAR014

Plasmid

#217026

Purpose

deletion of ior_operon

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_3620 (ycaO) and PP_3624

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pIAR015

Plasmid

#217027

Purpose

deletion of vanillic_acid_shunt

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_3353 and PP_3356 (fcs)

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pIAR001

Plasmid

#217016

Purpose

deletion of vdh

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequence of PP_3356 (fcs) and PP_3358 (ech)

Use

Bacterial gene replacement

Tags

none

Mutation

none

Promoter

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pIAR002

Plasmid

#217017

Purpose

deletion of aldB-I

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_0544 and PP_0546

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pIAR003

Plasmid

#217018

Purpose

deletion of PP_1948

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_1947 and PP_1949

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pIAR004

Plasmid

#217019

Purpose

deletion of areA(calA)

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_2425 and PP_5521

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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