We narrowed to 10,796 results for: HA

1. pHLmMBP-6

   Plasmid

   #72346

   Purpose

   Mammalian expression of cytoplasmic mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Use

   Tags

   6His-tag, HA-tag, Strep-tag II, and mMBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDF RBP-FL P53

   Plasmid

   #174042

   Purpose

   Express Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)

   Depositor

   Stewart Loh

   Article

   Blanden et al Elife. 2020 Dec 2;9. pii: 61487. doi: 10

   Insert

   P53 gene (TP53 Human)
   

   Use

   Tags

   Thermoanaerobacter Tencongenesis ribose binding p…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt

   Plasmid

   #85111

   Purpose

   expression of recombinant human mature (delta19) ETFalpha wt without tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta19) ETFalpha (ETFA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. SR140

   Plasmid

   #156037

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   SR140 (U2SURP Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. LZF1735 pAAV_hSyn-DiO-somArchon1-EGFP-P2A-somCheRiff

   Plasmid

   #126512

   Purpose

   Optopatch4. Simultaneous optical recording and manipulation of electrical activity in neurons

   Depositor

   Adam Cohen

   Article

   Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

   Insert

   somArchon1-EGFP-P2A-somCheRiff
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSG5 EDD

   Plasmid

   #37191

   Depositor

   Charles Watts, Darren Saunders

   Article

   Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

   Insert

   EDD (UBR5 Human)
   

   Use

   Tags

   6x His Tag and FLAG

   Expression

   Mammalian

   Mutation

   K503R (please see depositor comments below)

   Promoter

   SV40

   Available since

   Aug. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Syn1-DIO-VGLUT2:miniSOG

   Plasmid

   #214200

   Purpose

   For Cre-dependent expression of VGLUT2:miniSOG (miniSOG is lumen-facing and HA-tagged)

   Depositor

   Thomas Hnasko

   Article

   Steinkellner et al ACS Chem Neurosci. 2021 Feb 17;12(4):626

   Insert

   Slc17a6 tagged with miniSOG and hemagglutinin
   

   Use

   AAV and Cre/Lox

   Tags

   miniSOG (tagged with HA) is fused to the first in…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLEX-FHH-NRASQ61K-IRES-Puro

   Plasmid

   #120570

   Purpose

   Expresses Flag-HA-6xHIS-N-Ras Q61K fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   Flag-HA-6xHIS

   Expression

   Mammalian

   Mutation

   Q61K

   Promoter

   CMV

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-BG505.SOSIP-QES.i03.c01-8his

   Plasmid

   #111846

   Purpose

   Mammalian expression plasmid for soluble BG505 SOSIP.664; QES mutant with enhanced binding to PG16

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 Env (BG505 SOSIP.664)
   

   Use

   Tags

   8his purification tag and CD5 leader sequence

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; has SOSIP mutatio…

   Promoter

   CMV

   Available since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. THOC1

    Plasmid

    #156081

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    THOC1 (THOC1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTET2-lucCP-3MS2

    Plasmid

    #198351

    Purpose

    Part of Firefly reporter system, encodes Firefly luciferase with MS2 stem loops in the 3'UTR

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    Firefly Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTET2

    Available since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBABE-puro p21

    Plasmid

    #78783

    Purpose

    Retroviral expression of p21

    Depositor

    Jaewhan Song

    Article

    Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

    Insert

    p21 (CDKN1A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3

    Plasmid

    #170175

    Purpose

    Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2f-ST-P2A-NLS-mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLEX-FHH-NRAS-IRES-Puro

    Plasmid

    #120569

    Purpose

    Expresses Flag-HA-6xHIS-N-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTWIN1-His6-Ssp-Httex1-23Q

    Plasmid

    #84349

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon 1 (HTT Human)
    

    Use

    Tags

    N-terminal Ssp intein (His-tagged)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAC149-pCR8-dCas9VP160

    Plasmid

    #48221

    Purpose

    dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP160

    Expression

    Mutation

    D10A;H840A nuclease-deficient

    Promoter

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe puro H-Ras 61L

    Plasmid

    #13485

    Depositor

    Channing Der

    Article

    Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

    Insert

    HRas 61L (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    61L

    Promoter

    Available since

    Dec. 20, 2006

    Availability

    Academic Institutions and Nonprofits only

18. GDNF-SHC

    Plasmid

    #139624

    Purpose

    StrepIII-HA-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    GDNF (GDNF Human)
    

    Use

    Tags

    StrepIII-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHLmMBP-2

    Plasmid

    #72344

    Purpose

    Mammalian expression of secreted N-terminally 6His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Use

    Tags

    6His-tag, HA-tag, Strep-tag II, and mMBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. plenti-UbcP-IKZF3-3xHA-pGK-Pur

    Plasmid

    #69053

    Purpose

    lentiviral expression of IKZF3 with HA tag

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF3 (IKZF3 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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