We narrowed to 11,069 results for: aga

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Clone 16 - 3 HK02 pFUSE-IgG-human kappa VL-CH1 hole-clone2

Plasmid

#192185

Purpose

Clone 16 (HK02) pFUSE-IgG-human kappa VL-CH1 hole-clone2

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 2 light chain (HK02)

Expression

Mammalian

Mutation

NA

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-DENV_NS3-GS-NS2B

Plasmid

#201940

Purpose

Expresses DENV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

DENV NS3-GS-NS2B (POLY )

Expression

Yeast

Promoter

GAL1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-WNV_NS2B-GS-NS3

Plasmid

#203475

Purpose

Expresses WNV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

WNV NS2B-GS-NS3 (POLY )

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-SARS-CoV-2_PLP

Plasmid

#203509

Purpose

Expresses SARS-CoV-2 papain-like protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

SARS-CoV-2 PLP (ORF1ab )

Expression

Yeast

Promoter

GAL1

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pTBL469

Plasmid

#126458

Purpose

Expresses Cas9-15xFlag-TdT in mammalian cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

Cas9
15x Flag
TdT (DNTT Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75236

Purpose

CRISPR/Cas9 plasmid against human NFATc1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST53-LRRK2-ROC

Plasmid

#25053

Depositor

Mark Cookson

Article

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Contains only the ROC domain- aa 1333-1516

Available Since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
FLAG-KIF5A

Plasmid

#166954

Purpose

Expresses FLAG-tagged KIF5A protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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PRKAG1 gRNA (BRDN0001147838)

Plasmid

#77745

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAG1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAG1 (PRKAG1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK5 gRNA (BRDN0001145540)

Plasmid

#77542

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK5 (CDK5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hsChRmine-eYFP-WPRE

Plasmid

#183530

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

hsChRmine-eYFP

Use

AAV

Mutation

H33R

Promoter

CaMKIIa

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-DDX43-K292A

Plasmid

#134575

Purpose

Express human DDX43 mutant (K292A, ATPase dead) in E. coli

Depositor

Yuliang Wu

Article

Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

Insert

DDX43-K292A (DDX43 Human)

Tags

His

Expression

Bacterial

Mutation

full-length (K292A)

Promoter

T7

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCold I-Hero20

Plasmid

#187930

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero20

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero20 (C11orf58 Human)

Tags

Flag-6xHis

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75237

Purpose

CRISPR/Cas9 plasmid against human NFATc1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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PB-TA-ERP2-hcGAS(wt)

Plasmid

#208396

Purpose

To generate stable cell line expressing human cGAS(wt) using PiggyBac transposon system

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

Human cGAS (CGAS Human)

Use

Piggybac transposon

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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PB-TA-ERP2-hcGAS(R255A)

Plasmid

#208397

Purpose

To generate stable cell line expressing human cGAS(R255A) using PiggyBac transposon system

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

Human cGAS (CGAS Human)

Use

Piggybac transposon

Expression

Mammalian

Mutation

R255A

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-FbxO11-Jeff-pcDNA3.1-

Plasmid

#52503

Purpose

expresses human FbxO11-Jeff mutant in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

FbxO11 (FBXO11 Human)

Tags

FLAG-HA

Expression

Mammalian

Mutation

Q491L (Jeff)

Promoter

CMV

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-TGFB2

Plasmid

#185556

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting TGFB2

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

TGFB2 gRNAs

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pIS1 ATP2B1

Plasmid

#60788

Purpose

Luciferase reporter to measure miR-155 mediated differential repression. Contains ATP2B1 3' UTR and wild-type miR-155 sites

Depositor

David Bartel

Article

Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

Insert

ATP2B1 3'UTR and wild-type miR-155 binding site (ATP2B1 Human)

Use

Luciferase

Expression

Mammalian

Promoter

thymidine kinase (HSV-TK promoter)

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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KHK gRNA (BRDN0001145612)

Plasmid

#76982

Purpose

3rd generation lentiviral gRNA plasmid targeting human KHK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KHK (KHK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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