We narrowed to 9,323 results for: control

1. pCS2-MYC-GFP-IRES-mCherry

   Plasmid

   #231232

   Purpose

   MYC stability reporter construct (expressing c-myc 2) for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

   Depositor

   Michael Rape

   Article

   Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

   Insert

   MYC (MYC Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-MCRS1-GFP-IRES-mCherry

   Plasmid

   #231231

   Purpose

   MCRS1 stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

   Depositor

   Michael Rape

   Article

   Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

   Insert

   MCRS1 (MCRS1 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. SSH-pSUMO-β′-COPI-WD40-Tyr33Ala

   Plasmid

   #208170

   Purpose

   Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Tyr33Ala mutant (residues 1-301)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   N-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Tyr33Ala substitution
   

   Tags

   His-tag, SUMO-tag, and Strep-tag

   Expression

   Bacterial

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SSH-pcDNA3.1-α-COPI-WD40-Arg13Ala

   Plasmid

   #208172

   Purpose

   Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 Arg13Ala mutant (residues 1-327)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
   

   Tags

   Strep-tag

   Expression

   Mammalian

   Mutation

   Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SSH-pcDNA3.1-α-COPI-WD40-Arg300Ala

   Plasmid

   #208173

   Purpose

   Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg300Ala (residues 1-327)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys,Arg300Ala)
   

   Tags

   Strep-tag

   Expression

   Mammalian

   Mutation

   Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe19…

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SSH-pcDNA3.1-α-COPI-WD40-Lys15Ala

   Plasmid

   #208174

   Purpose

   Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Lys15Ala (residues 1-327)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
   

   Tags

   Strep-tag

   Expression

   Mammalian

   Mutation

   Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. JO-pH720-SARS-CoV-2-S-tail-WT

   Plasmid

   #218453

   Purpose

   Bacterial expression plasmid for the cytosolic tail of SARS-CoV-2 spike with an N-terminal eXact tag

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   Cytosolic tail of SARS-CoV-2 spike carrying an N-terminal eXact tag (S SARS-CoV-2)
   

   Tags

   eXact

   Expression

   Bacterial

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SSH-pcDNA3.1-SARS-CoV-2-S-Lys1269Ala-His1271Ala

   Plasmid

   #218463

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Lys1269Ala and His1271Ala substitutions)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   SARS-CoV-2 spike protein (1273 residues) carrying Lys1269Ala and His1271Ala substitutions (S )
   

   Expression

   Mammalian

   Mutation

   Lys1269Ala and His1271Ala substitutions

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-TRE-V5-TurboID-MUTT2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyA

   Plasmid

   #218915

   Purpose

   PiggyBac transposition

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-MUTT2A-SARS-CoV2-NSP7/BlastR (ORF1ab )
   

   Expression

   Mammalian

   Mutation

   2363G>C and 4330T>C

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-TRE-V5-TurboID-MUTT2A-SARS-CoV2-NCAP-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218913

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-MUTT2A-SARS-CoV2-NCAP/BlastR (N )
    

    Expression

    Mammalian

    Mutation

    2363G>C and 5335T>C

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NCAP-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218912

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-T2A-SARS-CoV2-NCAP/BlastR (N )
    

    Expression

    Mammalian

    Mutation

    2363G>C and 5335T>C

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-TRE-V5-TurboID-linker-MUTT2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218911

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-MUTT2A-huGR/BlastR (NR3C1 Human)
    

    Expression

    Mammalian

    Mutation

    2363G>C and 4155C>T and 4729T>G and 4731…

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC-hU6-shBACH1_BFP

    Plasmid

    #199217

    Purpose

    Expression plasmid with shRNAmiR targeting BACH1, with a BFP reporter on the backbone. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    shBACH1 (BACH1 Synthetic)
    

    Use

    RNAi

    Promoter

    human U6

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC_hU6-shRKIP_BFP

    Plasmid

    #199216

    Purpose

    Expression plasmid with shRNAmiR targeting RKIP (PEBP1), with a BFP reporter on the backbone.

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    shBACH1 (BACH1 Synthetic)
    

    Use

    RNAi

    Promoter

    human U6

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUt-mNF-GFP

    Plasmid

    #199220

    Purpose

    LPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    hTetR::P2A::EGFP
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV D2ir promoter

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)-R/G-ICUE

    Plasmid

    #181849

    Purpose

    ICUE cAMP sensor using sfGFP and mRuby2 as the FRET donor and acceptor.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    R/G-ICUE (RAPGEF3 Human)
    

    Tags

    mRuby2 and sfGFP

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. I282L

    Plasmid

    #184308

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I282L

    Promoter

    AOX1

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. I175L

    Plasmid

    #184322

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    I175L

    Promoter

    AOX1

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCSmEn2ERT(#117)

    Plasmid

    #184079

    Purpose

    cyclofen-inducible chicken EN2 (wt) activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    6his-myc-chkEn2-ERT2
    

    Use

    Synthetic Biology

    Tags

    6his-Myc

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available Since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCSmEn2n4ERT(#234)

    Plasmid

    #184082

    Purpose

    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    6his-myc-chkEn2(n4)-ERT2
    

    Use

    Synthetic Biology

    Tags

    6his-Myc

    Expression

    Mammalian

    Mutation

    chkEn2: G238S; ERT2: G400V, M543A, L544A, deleted…

    Promoter

    sCMV+SP6

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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