We narrowed to 9,109 results for: control

1. BD-pGEX5X3-BD-SARS-CoV-2-S-tail-Thr1273Asp

   Plasmid

   #218457

   Purpose

   Bacterial expression plasmid for the SARS-CoV-2 cytosolic tail (Thr1273Asp substitution) with an N-terminal GST tag

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   Cytosolic tail of SARS-CoV-2 spike carrying Thr1273Asp substitution and an N-terminal GST-tag (S SARS-CoV-2)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Thr1273Asp substitution

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-DnaJB1(H33Q)-PKAcat-mCherry

   Plasmid

   #181854

   Purpose

   mCherry-tagged DnaJB1-PKA catalytic subunit fusion with H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Histidine 33 in DnaJB1 changed to glutamine.

   Promoter

   CMV

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT2iApowriter2(#336)

   Plasmid

   #184104

   Purpose

   cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

   Insert

   myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
   

   Use

   Zebrafish transient transgenesis

   Tags

   internal tag: 5myc

   Expression

   Mutation

   DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

   Promoter

   Zf-Ubi

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT2iU6Apowriter2N(#355)

   Plasmid

   #184106

   Purpose

   cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis

   Depositor

   Sophie Vriz

   Article

   Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

   Insert

   myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
   

   Use

   Zebrafish transient transgenesis + in vitro trans…

   Tags

   internal tag: 5myc

   Expression

   Mutation

   DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

   Promoter

   Zf-Ubi+SP6

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT22Apowriter2(#340)

   Plasmid

   #184105

   Purpose

   cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

   Insert

   myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   internal tag: 5myc

   Expression

   Mutation

   DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

   Promoter

   Zf-Ubi

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT22iuasvApoWtEos3N(#486)

   Plasmid

   #184107

   Purpose

   cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

   Insert

   myr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   Expression

   Mutation

   DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

   Promoter

   4xUAS

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT22Ubm5En2SRE4PCh(#302)

   Plasmid

   #184089

   Purpose

   cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   5myc-chkEn2(SR)-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   5xMyc

   Expression

   Mutation

   chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …

   Promoter

   Zf-Ubi

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT22iuasvApoWChe(#488)

   Plasmid

   #184108

   Purpose

   cyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

   Insert

   myr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   Expression

   Mutation

   DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

   Promoter

   4xUAS

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Phage-PGK-Flag-HA-FKBP-T(G177D)

   Plasmid

   #181735

   Purpose

   for lentiviral expression of brachyury ORF in mammalian cells

   Depositor

   Charles Lin

   Article

   Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

   Insert

   T (brachyury)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   a silent mutation in the PAM site of an sgRNAt ta…

   Promoter

   Available since

   March 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pKHH030_sgCtrl#2

    Plasmid

    #174143

    Purpose

    Lentiviral vector expressing a control sgRNA that targets a safe harbor site

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX-EF1a_Empty

    Plasmid

    #221480

    Purpose

    Empty vector control for pLX307 overexpresion experiments

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT-IMD-V5-His

    Plasmid

    #63748

    Purpose

    Expresses drosophila IMD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

    Depositor

    Cyril Gueydan

    Insert

    immune deficiency (imd Fly)
    

    Use

    Tags

    V5-6XHis

    Expression

    Insect

    Mutation

    *see comment below

    Promoter

    pMT

    Available since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-fDIO-JRGECO1a

    Plasmid

    #128317

    Purpose

    Expresses JRGECO1a under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

    Depositor

    Patricia Jensen

    Has Service

    AAV1

    Insert

    NES-JRGECO1a
    

    Use

    AAV

    Tags

    His Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. XLone-Puro SPI1 P2A mCherry

    Plasmid

    #140029

    Purpose

    Tunable and temporal expression control of SPI1 and mCherry

    Depositor

    Xiaoping Bao

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. p667-UBC-GFP-V5_IDG-K

    Plasmid

    #135242

    Purpose

    Gateway destination clone of GFP (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    GFP-V5
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. XLone-BSD SPI1 P2A mCherry

    Plasmid

    #140031

    Purpose

    Tunable and temporal expression control of SPI1 and mCherry

    Depositor

    Xiaoping Bao

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. P48

    Plasmid

    #212155

    Purpose

    Plasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.

    Depositor

    Kang Zhou

    Insert

    superfolder green fluorescent protein, secondary alcohol dehydrogenase
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    PgyrA

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-CMV-Flag-GFP_IDG-K

    Plasmid

    #135275

    Purpose

    Gateway destination clone of GFP (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

    Depositor

    Ben Major

    Insert

    Flag-GFP
    

    Use

    Lentiviral; Gateway destination

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCI-6.20

    Plasmid

    #41002

    Depositor

    James Inglese

    Article

    Cheng et al Nat Methods. 2012 Oct;9(10):937. doi: 10

    Insert

    GSG-P2A-RLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Nov. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV-d2eGFP-cxcr4

    Plasmid

    #21967

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only