172,986 results
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Plasmid#86674PurposeExpresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.DepositorInsertGFP-FLAG-cGAS E225A/D227A
UseLentiviralAvailable SinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRL055 pDESTattB_cryaa:Venus
Plasmid#213509PurposeattB-containing pDEST plasmid, for use in Multisite Gateway reactionDepositorTypeEmpty backboneUseZebrafish expressionAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HaloKbp1b
Plasmid#228794PurposeChemigenetic indicator for potassium ion - low affinityDepositorInsertHaloKbp1b
TagsHis-TagExpressionBacterialAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-pegRNA-puro backbone
Plasmid#214085PurposeLentiviral backbone plasmid to insert pegRNAs of interest. The cloning site is BsmbI.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterhU6, EF1aAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-Cas9
Plasmid#181752PurposeMMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.DepositorInsertMMLV-Gag-3xNES-Cas9 (Spy)
TagsFlagExpressionMammalianPromoterCMVAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
mApple-Alpha-V-Integrin-25
Plasmid#54866PurposeLocalization: Focal Adhesions, Excitation: 568, Emission: 592DepositorAvailable SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET16B.Pfu
Plasmid#12509PurposeBacterial expression of Pfu DNA polymeraseDepositorInsertPfu DNA polymerase
ExpressionBacterialMutationY218H, Q462R, F533L, K559I and V683A (mutations w…Available SinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGL3b-8XGliBS:EGFP
Plasmid#84602PurposeGli protein-dependent EGFP reporter for analysis of Hedgehog signalingDepositorInserteGFP
ExpressionMammalianPromoter8X Gli-binding siteAvailable SinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
TETv4
Plasmid#167983PurposeExpresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoterDepositorInsertTETv4 (TET1CD-XTEN80-dCas9)
UseCRISPR and Synthetic BiologyTags3xNLS, TET1 catalytic domain, and tagBFPExpressionMammalianPromoterCAGAvailable SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
Plasmid#60903PurposeExpressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activationDepositorInsertCas9 dead
UseCRISPR and LentiviralExpressionMammalianAvailable SinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only