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Plasmid#168902PurposeGateway-compatible Entry vectorDepositorInsertHCoV-HKU1-Hemagglutinin-esterase (HE )
UseGateway-compatible entry vectorTagsExpressionMutationCodon optimized (H.sapiens)PromoterAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMCB320 sgControl (ACOCB)
Plasmid#228115PurposeSafe targeting sgRNADepositorInsertSafe targeting sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMCB320 sgControl (GEA)
Plasmid#228144PurposeSafe targeting sgRNADepositorInsertSafe targeting sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
STp6
Plasmid#235740PurposeProtein expression of human RAB5A Q79,C19S,C63S,S84TAG, 1-212-His6 in bacteria for unnatural amino acid incorporationDepositorInsertRAB5A (RAB5A Human)
UseTags6xHisExpressionBacterialMutationQ79, C19S, C63S, S84TAG, aa 1-212PromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJB78
Plasmid#235738PurposeProtein expression of GST-TEV-HsRAB1A Q70L C26S C126S in bacteria for maleimide conjugationDepositorInsertRAB1A (RAB1A Human)
UseTagsGST-TEVExpressionBacterialMutationQ70L C26S C126S, aa 1-204PromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD D262V
Plasmid#234607PurposeBacterial expression of N-terminally 6His tagged hnRNPA1 D262V LCD (186-320)DepositorInserthnRNPA1_LCD_D262V (HNRNPA1 Human)
UseTags6xHis-TEVExpressionBacterialMutationC-terminal domain (186-320) bearing familial ALS …PromoterT7Available sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD D262N
Plasmid#234608PurposeBacterial expression of N-terminally 6His tagged hnRNPA1 D262N LCD (186-320)DepositorInserthnRNPA1_LCD_D262N (HNRNPA1 Human)
UseTags6xHis-TEVExpressionBacterialMutationC-terminal domain (186-320) bearing familial ALS …PromoterT7Available sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD -3G1S+4V
Plasmid#234612PurposeBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 Gly and 1 Ser mutated to AsnDepositorInserthnRNPA1_LCD_4GSV (HNRNPA1 Human)
UseTags6xHis-TEVExpressionBacterialMutationG204V, S231V, G274V, G303VPromoterT7Available sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
p5E-Dll4-F2-E1B-B-Globin (JDW 1366)
Plasmid#229843PurposeA gateway compatible 5' entry clone containing the murine Dll4 F2 arterial specific enhancer upstream of a minimal E1b promoter and intron for arterial and endocardial expression.DepositorInsertDll4-F2 (exon3)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.BC(p1-10)-hDLXI56i-minBG-CI-EGFP-W3SL-BC(p1-10)
Plasmid#231360PurposeSingle stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with hDLXI56i enhancer.DepositorInsertBC(p1-10)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.BC(p1-10)-mscRE4-minBG-CI-EGFP-W3SL-BC(p1-10)
Plasmid#231361PurposeSingle stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with mscRE4 enhancer.DepositorInsertBC(p1-10)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.minBG-EGFP-W3SL_BbsI(GGA)
Plasmid#231362PurposeminBG-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.DepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Ple155-CI-EGFP-W3SL_BbsI(GGA)
Plasmid#231363PurposePle155-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.DepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)
Plasmid#231364PurposePle155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.DepositorInsertmRuby2
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)
Plasmid#231367PurposePle155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequenceDepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6-sasgRNA(SapI)_Ple155-CI-mRuby2-W3SL_BbsI(GGA)
Plasmid#231368PurposePle155-driven mRuby2, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequenceDepositorInsertmRuby2
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.BC(p1-10)-Ple155-CI-EGFP-W3SL-BC(p1-10)
Plasmid#231351PurposeSingle stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from Ple155 element, active in Purkinje cells.DepositorInsertBC(p1-10)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.BC(p1-10)-CMVe-SCP1-TagBFP2-W3SL-BC(p1-10)
Plasmid#231354PurposeSingle stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter and CMV enhancer.DepositorInsertBC(p1-10)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.BC(p1-10)-SCP1-TagBFP2-W3SL-BC(p1-10)
Plasmid#231355PurposeSingle stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter.DepositorInsertBC(p1-10)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)
Plasmid#231345PurposeSingle stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.DepositorInsertT7/T3-BC(p1-14)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only