We narrowed to 10,393 results for: plasmids 101

1. pHR_PGK_SNIPR_CD8alpha variant 2

   Plasmid

   #188362

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nrxn3b-Nav1.6-MKII-f-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

   Plasmid

   #163031

   Purpose

   FLiCRE TF with fast kinetics for neuronal expression

   Depositor

   Alice Ting

   Article

   Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

   Insert

   Nrxn3b-MKII-f-hLOV1-TEVcs-tTA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CROPseq-tRFP

   Plasmid

   #230938

   Purpose

   Modified version of the original CROP-seq vector (Addgene #86708), expressing tRFP instead of puroR for FACS

   Depositor

   Hyejung Won

   Article

   Lee et al Cell. 2025 Jan 22:S0092-8674(24)01435-1.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagRFP

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas9D10A_GFP

   Plasmid

   #44720

   Purpose

   Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9D10A-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   D10A mutation- nickase mutant

   Promoter

   CAG

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. APEX2-GBP

   Plasmid

   #67651

   Purpose

   Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   APEX2-GBP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAT009

   Plasmid

   #171635

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-B2M
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. TL026_AAV_hSyn:SEP-GluA1

   Plasmid

   #192519

   Purpose

   Expression of AMPA GluA1 with N terminal super ecliptic phluorin (SEP) fusion

   Depositor

   Adam Cohen

   Article

   Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

   Insert

   SEP-GluA1
   

   Use

   AAV and Cre/Lox

   Tags

   super ecliptic phluorin

   Promoter

   hSyn

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. CaMKIIP-mEGFP-P2A-paAIP2 (R5AR6A)/pAAV

   Plasmid

   #91719

   Purpose

   AAV-mediated expression of mEGFP and paAIP2 (R5AR6A), the functional mutant of paAIP2

   Depositor

   Ryohei Yasuda

   Article

   Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

   Insert

   mEGFP-P2A-paAIP2(R5AR6A)
   

   Use

   AAV

   Promoter

   CaMKII 1.3

   Available Since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. mAIRN HO

   Plasmid

   #207411

   Purpose

   Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

   Depositor

   Karlene Cimprich

   Article

   Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

   Insert

   mAIRN
   

   Expression

   Bacterial and Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. mAIRN CD

    Plasmid

    #207412

    Purpose

    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    mAIRN
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. ECFP HO

    Plasmid

    #207413

    Purpose

    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    ECFP
    

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. ECFP CD

    Plasmid

    #207414

    Purpose

    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    ECFP
    

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSMPP-mCherry-mTrim21

    Plasmid

    #104971

    Purpose

    Lentiviral Transfer Plasmid with mCherry-tagged mouse Trim21

    Depositor

    Leo James

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Tripartite Motif containing 21 (Trim21 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Promoter

    SFFV

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE

    Plasmid

    #203842

    Purpose

    Flp-dependent Cre and EGFP expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    Cre
    

    Use

    AAV

    Tags

    EGFP-2A

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ132A2.0

    Plasmid

    #99887

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU6

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ133A2.0

    Plasmid

    #99891

    Purpose

    Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU6

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMC_mNG2(11)_BSDminus_F2

    Plasmid

    #184164

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mNG2(11)
    bsd
    

    Use

    CRISPR

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR_PGK_SNIPR_CD8alpha variant 4

    Plasmid

    #188364

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant4-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant4-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR_PGK_SNIPR_CD8alpha variant 2, G318A

    Plasmid

    #188365

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_G318A, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_G318A_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. RFP-(SUMO)3

    Plasmid

    #127150

    Purpose

    Mammalian expression plasmid for mCherry-(SUMO)3 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    3 copies of human SUMO1 separated by (GGS)4 (SUMO3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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