We narrowed to 15,732 results for: LEA

1. pCasper-pNos-PCP-eGFP

   Plasmid

   #180274

   Purpose

   to epress PCP-GFP fusion protein in the early fly embryos (driven by maternal nanos driver)

   Depositor

   Hernan Garcia

   Article

   Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

   Insert

   PCP-eGFP
   

   Tags

   eGFP

   Expression

   Insect

   Promoter

   nanos

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pg-dsRedXP-HIF-2alpha

   Plasmid

   #87209

   Purpose

   N-terminally dsRedXP-tagged human HIF-2a/EPAS

   Depositor

   Violaine Sée

   Article

   Taylor et al Open Biol. 2016 Sep;6(9). pii: 160195.

   Insert

   HIF-2a (EPAS1 Human)
   

   Tags

   dsRedXP

   Expression

   Mammalian

   Mutation

   Q868E

   Promoter

   CMV

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBK.009

   Plasmid

   #187209

   Purpose

   Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG]), and Cas1 + Cas2

   Depositor

   Seth Shipman

   Article

   Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

   Inserts

   Retron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG])
   Cas1 + Cas2
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7/Lac

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 PARL-FLAG-CT S65A+T69A+S70A

   Plasmid

   #13616

   Depositor

   Luca Pellegrini

   Article

   Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

   Insert

   Presenilin-associated rhomboid-like (PARL, PARL Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Ser 65, Thr 69 and Ser 70 to Alanine

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

5. OVA-miSFIT-2xPerfect

   Plasmid

   #124673

   Purpose

   Tuning expression of cytosolic Ovalbumin coupled to EGFP

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   Cyto-Ovalbumin
   

   Expression

   Mammalian

   Mutation

   Truncated OVA for cytoplasmic expression

   Promoter

   minimal CMV

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-Map7-Start-1351

   Plasmid

   #46075

   Depositor

   Edgar Gomes

   Article

   Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

   Insert

   Map7-Start-1351 (Map7 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. RGG-mCherry-RGG-eDHFR

   Plasmid

   #164648

   Purpose

   Two RGG domains tagged with mCherry and eDHFR

   Depositor

   Huaiying Zhang, Michael Lampson

   Article

   Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

   Insert

   RGG
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI syn iGluf

   Plasmid

   #106121

   Purpose

   Fast indicator for synaptic glutamate imaging (synapsin promoter)

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR E25D variant
   

   Tags

   Myc and PDGFR

   Expression

   Mammalian

   Mutation

   Changed Glu 25 to Asp

   Promoter

   synapsin

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nanog-CreER targeting construct

   Plasmid

   #59720

   Purpose

   Cre-ER knockin targeting construct for mouse endogenous Nanog locus

   Depositor

   Jacob Hanna

   Article

   Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

   Insert

   creERT2
   

   Use

   Mouse Targeting

   Promoter

   mNanog

   Available Since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSC-NF8-d1-eGFP

    Plasmid

    #122036

    Purpose

    AAV vector, NFkappaB-dependent eGFP expression

    Depositor

    Liliane Tenenbaum

    Article

    Chtarto et al PLoS One. 2013;8(1):e53156. doi: 10.1371

    Insert

    eGFP
    

    Use

    AAV

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHJ425

    Plasmid

    #20683

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Ezrin (EZR Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Lys 253, 254, 262, 263 to Asn; and change…

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pIS1 DICER1 long-mut-let-7

    Plasmid

    #21651

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutant let-7 microRNA binding sites

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

    Plasmid

    #112211

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    C-terminus of dCas9
    

    Use

    AAV

    Mutation

    D10A

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. NG1237 pCI-TPI-PTC160-4H

    Plasmid

    #65804

    Purpose

    NMD reporter mRNA

    Depositor

    Niels Gehring

    Article

    Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

    Insert

    TPI (TPI1 Human)
    

    Tags

    4H (4 copies of short beta-globin 3'UTR for …

    Expression

    Mammalian

    Mutation

    premature stop codon at 160

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOCC94

    Plasmid

    #118912

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease and eGFP-HIS6

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. MDH1-miR-222-PGK-GFP-Blast

    Plasmid

    #31522

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-222 (Mir222 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pMal-SLFN2

    Plasmid

    #174322

    Purpose

    Bacterial expression of MBP-tagged mouse SLFN2

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    SLFN2 (Slfn2 Mouse)
    

    Tags

    MBP

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. VRER variant

    Plasmid

    #138561

    Purpose

    Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRER
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. IBMc326

    Plasmid

    #161949

    Purpose

    Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on ECF20

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter
    

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIS1 DICER1 long-mut-all

    Plasmid

    #21653

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutated let-7 and miR103/107 microRNA binding sit…

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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