We narrowed to 11,743 results for: pcdna

1. pCDNA3 Flag p38 gamma (agf)

   Plasmid

   #20354

   Depositor

   Roger Davis

   Article

   Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

   Insert

   pDCNA3 Flag p38 gamma(agf) (Mapk12 Rat)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Replace Thr 183 and Tyr 185 with Ala and Phe resp…

   Promoter

   Available since

   April 29, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA5/FRT/TO SBBP-CDK1 WT (2007)

   Plasmid

   #61847

   Purpose

   mammalian expression of CDK1 WT, used for Ips

   Depositor

   Jonathon Pines

   Insert

   CDK1 (CDK1 Human)
   

   Use

   Tags

   SBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA-Cas9-T2A-STOP-TdT

   Plasmid

   #126425

   Purpose

   Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-T2A-STOP-TdT (DNTT Streptococcus pyogenes, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   alanine-6 of TdT has been changed to a STOP codon

   Promoter

   pCMV

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-R536C-WLS-HA-IRES-GFP

   Plasmid

   #178065

   Purpose

   Expression of WLS-HA protein (R536C mutant)

   Depositor

   Joseph Gleeson

   Article

   Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

   Insert

   WLS (WLS Human)
   

   Use

   Tags

   HA and IRES-GFP

   Expression

   Mammalian

   Mutation

   R536C

   Promoter

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-bJun-C-mSOG-IRES-mCherry

   Plasmid

   #136600

   Purpose

   Encodes bJun domain tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

   Depositor

   John Ngo

   Article

   Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

   Insert

   bJun-C-mSOG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-ELYS-emerin-EGFP-polyA

   Plasmid

   #59745

   Purpose

   Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   ELYS-emerin-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Flag mTOR S2035T R2109A

   Plasmid

   #26606

   Depositor

   Jie Chen

   Article

   Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

   Insert

   mTOR (MTOR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S2035T R2109A

   Promoter

   Available since

   Nov. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1 SARS-CoV-2 S D614-trunc

   Plasmid

   #158076

   Purpose

   Expresses SARS-CoV-2 spike protein with a truncation

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5 FRT TO Myc ArC Long WT

   Plasmid

   #59809

   Purpose

   Allows the integration of Myc ArC Long in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

   Insert

   Aurora C (AURKC Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 GIL-11 myc TEV TwinStrep

    Plasmid

    #199627

    Purpose

    Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

    Depositor

    Jurgen Scheller

    Article

    Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

    Insert

    GIL-11
    

    Use

    Tags

    TwinStrep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA 3.1 (+) FLAG-Ago2 S387E

    Plasmid

    #60653

    Purpose

    Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    CMV-FLAG-Ago2 (N-term tag)

    Expression

    Mammalian

    Mutation

    S387E mutant

    Promoter

    CMV

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Myc3-Cul3 H2M/H5M

    Plasmid

    #21591

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

    Insert

    cullin 3 (CUL3 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Mutated residues H2M/H5M

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

13. Nano-lantern(Ca2+)_300/pcDNA3

    Plasmid

    #51983

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-3GS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-CMV-CIBN-mCherry-VAMP2

    Plasmid

    #178573

    Purpose

    Mammalian expression plasmid of CIBN-VAMP2 of Opto-vTrap

    Depositor

    Won Do Heo

    Article

    Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

    Insert

    CIBN-mCherry-VAMP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5 FRT TO myc Separase C2029A

    Plasmid

    #59822

    Purpose

    Allows the integration of myc Separase C2029A in the genome and Tet-inducible expression

    Depositor

    Stephen Taylor

    Article

    Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    C2029A

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. XE177 Xenopus Wnt-8-myc-pcDNA3

    Plasmid

    #16863

    Depositor

    Randall Moon

    Insert

    Wnt-8 (wnt8a.L Frog)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1 FLIPglu-30uDelta13V ECFP-W67G

    Plasmid

    #18017

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    FLIPglu D154A Delta13V ECFP-W67G
    

    Use

    Tags

    ECFP-W67G and Venus

    Expression

    Mammalian

    Mutation

    mglB-D154A; non-functional ECFP

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-RIPK3[S199D][S227D]-FLAG

    Plasmid

    #199324

    Purpose

    double mutated phosphorylation site (kinase acitivity and MLKL interaction active)

    Depositor

    Adrian Press

    Article

    Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

    Insert

    Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 199 and 227 to Aspartic Acid

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA4TO-24xGCN4_v4-kif18b-Xbp1-24xPP7

    Plasmid

    #74931

    Purpose

    Translation reporter with Xbp1 pause site

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    24xGCN4_v4-kif18b-Xbp1-24xPP7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA4-RI1-332-MYC-His6

    Plasmid

    #185351

    Purpose

    Mammalian expression of Ribophorin I

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    Ribophorin I (RPN1 Human)
    

    Use

    Tags

    Myc, 6xHis

    Expression

    Mammalian

    Mutation

    aa 1-332 only

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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