We narrowed to 6,763 results for: poly
-
Plasmid#109768PurposeCodon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract.DepositorInsertTol2 Transposase
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
DCAF1:PBC020-D05:C246337
Plasmid#183974PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertDCAF1:PBC020-D05:C246337 (DCAF1 Human)
UseBaculovirus expresssionTags6xHis-TEV and MHHHHHHSSGRENLYFQGPromoterPolyhedrinAvailable SinceMay 10, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
p2,0_3E5E_eGFP
Plasmid#69359PurposeEncodes for Ebola Minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoterDepositorInsert3E5E eGFP minigenome
UseT7PromoterT7Available SinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas11
Plasmid#178882PurposeExpression of human codon-optimized NlaCas11 with N terminal NLS and HA tagDepositorInsertEF1a-NlaCas11c-bGH polyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDctp-mSox10-Mp1
Plasmid#13513DepositorAvailable SinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_T3_ABEmax_IVT
Plasmid#171761PurposePlasmid to be used as DNA template for in-vitro RNA transcription of the ABEmax base editor (A to G) by T3 RNA polymeraseDepositorInsertABEmax
UseVector for in-vitro transcriptionPromoterT3 promoterAvailable SinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
RNF168-Halo HRD
Plasmid#207080PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
ExpressionMammalianPromoterNoneAvailable SinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual
Plasmid#216377PurposeBaculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)DepositorInsertLDL receptor related protein 6 (LRP6 Human)
UseBaculovirusTags9xHis and HA signal sequence-FLAG-3C cleavage sit…ExpressionInsectPromoterPolyhedrin (LRP6)/p10 (MESD)Available SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-V96 ELP
Plasmid#67853PurposeGFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residuesDepositorInsertGFP fused to ELP (VPGVG) repeated 96 times
TagsGFP fused to N-terminal of ELPExpressionMammalianPromoterT7Available SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_T3_BE4_IVT
Plasmid#171762PurposePlasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymeraseDepositorInsertBE4
UseVector for in-vitro transcriptionPromoterT3 promoterAvailable SinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Clathrin-LCa-ECFP
Plasmid#21742DepositorAvailable SinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
hPDI1-18-508_C400S_C56S-pFLAG-CMV1
Plasmid#31383DepositorInsertPDI1A (P4HB Human)
TagsFLAG M2ExpressionMammalianMutationC-terminal cysteines of both active sites mutated…Available SinceMay 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-hCasD10A-pA
Plasmid#51638PurposeExpresses Cas9-nickase in mammalian cells and zygotes.DepositorInsertCodon-optimized Cas9 D10A
UseCRISPRTagsFLAG, NLS, and polyAExpressionMammalianPromoterCAG promoterAvailable SinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCas1_Cas2/3
Plasmid#89240PurposePlasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1DepositorInsertCas1 and Cas2/3
TagsStrep II tag on Cas1Available SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
SP6-hCas9-Ce-mRNA
Plasmid#47911PurposeIn vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.DepositorInserthCas9 with C. elegans 5' and 3' UTRs
UseCRISPRPromoterSP6Available SinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
MX01
Plasmid#158624PurposeGATEWAY and BacMam compatible mammalian expression vector with eGFP expression reporter (eGFP is expressed by same promotor but as a separate polypeptide chain).DepositorTypeEmpty backboneUseGateway destination vector ; bacmam acceptor vect…TagsP2AExpressionMammalianPromoterCMV-IEAvailable SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
TAF1
Plasmid#39118PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
3922-48
Plasmid#176497PurposepTRE-EGFP-T2A-TFEB(wt)-polyADepositorInsertEGFP-T2A-TFEB(wild-type; full-length; human)
ExpressionMammalianPromoterTREAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP
Plasmid#112631PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral RGDSP integrin-binding sequencePromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
rat APOBEC1
Plasmid#112858Purposeplasmid for expression of rat APOBEC1 in mammalian cellsDepositorAvailable SinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only