We narrowed to 18,914 results for: Tec

1. pBK-YE1-BE3

   Plasmid

   #85174

   Purpose

   Expresses YE1-BE3 in mammalian cells

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   YE1-BE3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCellFree_G03 IL6

   Plasmid

   #67061

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   IL6 (IL6 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. EK0379 CMV-TO-EGFP-stop-t70587 (PB)

   Plasmid

   #191154

   Purpose

   Expression of Trigger 1 in the 3' UTR of EGFP in mammalian cells; used to make a cell line to study sensors for integrated 3' UTR or CDS triggers; expression is inducible with doxycycline due to CMV-TO promoter.

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   EGFP-t70587
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV-TO

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL

   Plasmid

   #169533

   Purpose

   Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven ER-localized monomeric red fluorescent protein mRuby2
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-OSF-VPS15

   Plasmid

   #99326

   Purpose

   Component for mammalian expression of recombinant PI3KC3-C1 complex

   Depositor

   James Hurley

   Article

   Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

   Insert

   VPS15 (PIK3R4 Human)
   

   Tags

   Strep-Strep-Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

6. GB4542

   Plasmid

   #215261

   Purpose

   Module for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   CBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. GB4401

   Plasmid

   #215252

   Purpose

   Module for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   SF-PNOS:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGWB516

   Plasmid

   #74858

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKD233.7-3

   Plasmid

   #90955

   Purpose

   Expresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutively

   Depositor

   Jeffrey Tabor

   Article

   Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

   Inserts

   Tetrathionate response regulator
   Superfolder GFP
   mCherry
   

   Expression

   Bacterial

   Promoter

   Bba_J23114, PltetO-1, and PttrB185-269

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEC3079 (p7INT-Pveg_lacI)

    Plasmid

    #218505

    Purpose

    integrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    lacI (lacI Escherichia coli)
    

    Expression

    Bacterial

    Promoter

    Pveg

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMIII-Neo-BFP

    Plasmid

    #153426

    Purpose

    Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    mTagBFP2
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. attP-puro-GA donor

    Plasmid

    #182140

    Purpose

    attP-puro-GA DNA donor for twinPE and Bxb1 mediated gene-size insertion

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attP-GA-EGFP-EF1α-PuroR-T2A-TagBFP
    

    Expression

    Mammalian

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. GWF243

    Plasmid

    #133608

    Purpose

    NS3a-CAAX-(IRES)-EGFP-DNCR2-TIAM-P2a-BFP-GNCR1-LARG

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    NS3a-CAAX, EGFP-DNCR2-TIAM, BFP-GNCR1-LARG
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-blueCKAR

    Plasmid

    #118472

    Purpose

    cpBFP-based single-color biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueCKAR
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3×Cherry Dam AID Smash

    Plasmid

    #227374

    Purpose

    For inducible expression and cytosolic localization of Dam

    Depositor

    David Pellman

    Article

    Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

    Insert

    Dam DNA-adenine methyltransferase
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AA294

    Plasmid

    #215968

    Purpose

    Fragmid fragment: (Pol 2 promoter) U1 small nuclear RNA promoter

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U1a_v1
    

    Use

    Fragment

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AA332

    Plasmid

    #216053

    Purpose

    Fragmid fragment: (C' terminus) 3x NLS sequences

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    SV40NLS_v1.10; SV40NLS_v1.11; SV40NLS_v1.12
    

    Use

    Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTS2340_Tier3(SB)-iRFP_Puro

    Plasmid

    #169638

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven iRFP670 and PuroR expression
    

    Expression

    Mammalian

    Promoter

    PRPBSA

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJS646

    Plasmid

    #183175

    Purpose

    Y2H vector compatible with pJS647 PYR1 insert

    Depositor

    Timothy Whitehead

    Article

    Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMV_AA089

    Plasmid

    #216105

    Purpose

    gRNA expression for Cas12a (guide only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    gRNA expression
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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