We narrowed to 14,124 results for: flag

1. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A

   Plasmid

   #51247

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   amino acids 849,865,877,907 mutated to Ala

   Promoter

   EF1a

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)

   Plasmid

   #133790

   Purpose

   CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized NmeCas9
   

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CAG

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKK-BI16-ORF1-3C-FLAG_ORF2-TEV-mClover3

   Plasmid

   #105803

   Purpose

   Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C protease; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   ORF1: 3C-FLAG; ORF2: TEV-mClover3

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5FRT/Flag-hINO80 HSA (212-526)

   Plasmid

   #29443

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

   Insert

   INO80 (INO80 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   INO80 212-526 (a.a.)

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

5. HA-mRIPK3 D143N-Flag/cHA-pTRIPZ vector

   Plasmid

   #73703

   Purpose

   lentiviral expression of mRIPK3 mutant

   Depositor

   Francis Chan

   Insert

   RIPK3 (Ripk3 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   D143N

   Promoter

   CMV/tet

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pcdna3 Flag Jip3b(T266A, T276A, T287A)

   Plasmid

   #58924

   Purpose

   interacts with MAPK signaling module

   Depositor

   Roger Davis

   Article

   Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

   Insert

   mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed T266A, T276A, T287A

   Available Since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST

   Plasmid

   #127316

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

   Plasmid

   #113162

   Purpose

   A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   SpCas9
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG

   Available Since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4/TO-myc NES-mCE NLS-Flag

   Plasmid

   #82468

   Purpose

   Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme

   Depositor

   Daniel Schoenberg

   Article

   Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

   Insert

   Myc-NES-mCE-Flag (Rngtt HIV, Mouse)
   

   Tags

   Flag and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDAX-018 FLAG Venus210[1] IRES Venus210[2]

    Plasmid

    #130128

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    Venus
    

    Expression

    Mammalian

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3 Flag MKK3b(Glu, delete LXL)

    Plasmid

    #50450

    Purpose

    activates p38 in the signaling pathway

    Depositor

    Roger Davis

    Article

    Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

    Insert

    MKK3b (MAP2K3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted L25-I27, changed Serine 218 to Glutamic A…

    Promoter

    CMV

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

12. Lentiviral FLAG-CaMKKbeta S129D, S133D, S137D

    Plasmid

    #32460

    Depositor

    Anthony Means

    Article

    Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    S129D, S133D, S137D

    Promoter

    human PGK

    Available Since

    Feb. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pNIA-CEN-Venus-FLAG-LINXa4-Bre1

    Plasmid

    #73618

    Purpose

    Visualizing the nuclear translocation of LINXa4-Bre1 in yeast.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    Venus
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A

    Promoter

    ADH1

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-Tagged Clavesin1 (pCMV-Tag2B-Sec14a)

    Plasmid

    #47430

    Depositor

    Peter McPherson

    Article

    Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

    Insert

    Clavesin1 (CLVS1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127318

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 FLAG mTOR 1968-2549 S2035T

    Plasmid

    #23000

    Depositor

    Jing Huang

    Article

    Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

    Insert

    mTOR (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S2035T aa 1968-2549 only

    Available Since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e

    Plasmid

    #90011

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Cdc25C S216A
    NBirA*-14-3-3e
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Ser 216 to Ala

    Promoter

    CMV

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLIB_3xFlag-His-PLpro (nsp3) (SARS-CoV-2)

    Plasmid

    #169194

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 papain-like protease domain (nsp3) in insect cells

    Depositor

    John Diffley

    Article

    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

    Insert

    3xFlag-His6-PLpro (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    3xFlag-His6

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-Tagged Clavesin2 (pCMV-Tag2B-Sec14b)

    Plasmid

    #47431

    Depositor

    Peter McPherson

    Article

    Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

    Insert

    Clavesin2 (CLVS2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX

    Plasmid

    #112762

    Purpose

    express GFP-4EBP1-Rheb15(CAAX mutation)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-GFP-4EBP1-Rheb15 (CAAX mutant) (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Rheb15(CAAX mutant)

    Mutation

    CAAX mutation to prevent lipid localization

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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