We narrowed to 9,384 results for: Coli

1. pRSETA_crE6G2

   Plasmid

   #172929

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain

   Depositor

   Arnold Boersma

   Article

   Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

   Insert

   CrowdingSensorE6G2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Val317Gly, Met316Gly

   Promoter

   T7

   Available since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PibpA GFP pZa

   Plasmid

   #170076

   Purpose

   GFP expression under the control of E. coli ibpA promoter

   Depositor

   Urartu Seker

   Article

   Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

   Insert

   Green fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PibpA

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PfxsA GFP pZa

   Plasmid

   #170075

   Purpose

   GFP expression under the control of E. coli fxsA promoter

   Depositor

   Urartu Seker

   Article

   Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

   Insert

   Green fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PfxsA

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMD19T-slr0230-PL22-sgRNANT1-KmR

   Plasmid

   #73224

   Purpose

   Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli

   Depositor

   Paul Hudson

   Article

   Yao et al ACS Synth Biol. 2015 Dec 28.

   Insert

   PL22-sgRNA NT1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PL22

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTH1-SaSrtA-Q

   Plasmid

   #64980

   Purpose

   Expresses the E108Q mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, E108Q

   Promoter

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMZ2007

   Plasmid

   #204399

   Purpose

   A 3OC6-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102

   Inserts

   quorum sensing regulator LuxR
   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PftsH and Synthetic promoter PLux0002

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEVA221TrxA-AID-T7RNAP

   Plasmid

   #167976

   Purpose

   Expresses fusion protein TrxA-AID-T7RNAP in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

   Insert

   TrxA-AID-T7RNAP
   

   Use

   Tags

   T7RNAP and TrxA

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmMaple2-CAM

   Plasmid

   #101149

   Purpose

   Fluorescent protein for superresolution imaging

   Depositor

   Xiaowei Zhuang

   Article

   Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

   Insert

   E. coli codon optimized mMaple2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CHIKV-nsP3-c001

   Plasmid

   #173086

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   N/A
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   A1334-T1493, codon optimized

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Plas-gRNA-LEU2

    Plasmid

    #157656

    Purpose

    GFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequence

    Depositor

    Frank Albert

    Article

    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

    Insert

    GFP-gRNA
    

    Use

    Insert storage (replicative in e. coli)

    Tags

    GFP-gRNA

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. p70rg

    Plasmid

    #17827

    Depositor

    Jay Keasling

    Article

    Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

    Insert

    E. coli optimized red flourescent protein (RFPec), GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pSecEffector

    Plasmid

    #172891

    Purpose

    Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901

    Depositor

    Urartu Seker

    Article

    Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

    Inserts

    Bxb1
    TP901
    TEV Display cassette
    ProD promoter between anti aligned Bxb1 sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ProD, nTETO, and pBAD /AraC

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-nGFPMNase

    Plasmid

    #187826

    Purpose

    express nGFPMNase protein in E coli

    Depositor

    Yikang Rong

    Article

    Stefanis et al Brain Res Mol Brain Res. 1998 Jan;53(1-2

    Insert

    nGFPMNASE
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promotor

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIA1405

    Plasmid

    #172404

    Purpose

    Expression of SARS-COV-2 Nsp12 D218A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    D218A

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIA1420

    Plasmid

    #172406

    Purpose

    Expression of SARS-COV-2 Nsp12 R116A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    R116A

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIA1421

    Plasmid

    #172407

    Purpose

    Expression of SARS-COV-2 Nsp12 K50A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pICH75111

    Plasmid

    #50327

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, beta-glucuronidase (E. coli) with two introns,
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBXPC3H-Syb37

    Plasmid

    #123627

    Purpose

    Expresses Syb37 in e. coli cells, with Arabinose induction and Ampicillin selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    Syb37
    

    Use

    Tags

    3C-His and PelB

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEXP5-NT/pLux LasI

    Plasmid

    #193628

    Purpose

    Inducible expression of LasI by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL). LasI is codon optimized for E. coli.

    Depositor

    Dora Tang

    Article

    Gonzales et al ChemSystemsChem 2023

    Insert

    LasI
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Lux promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTYF-1xSYN-tTA

    Plasmid

    #19980

    Depositor

    Sergey Kasparov

    Article

    Liu et al J Gene Med. 2008 May . 10(5):583-92.

    Insert

    SYN-tTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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