We narrowed to 30,707 results for: PLE

Showing: 8821 - 8850 of 30707 results

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pDGE2

Plasmid

#79441

Purpose

recipient plasmid [multiplexing]; p35S:Cas9, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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Lf-MetQ

Plasmid

#78305

Purpose

Methionine Binding Protein (MetQ) with C-Terminal 6x Histidine Tag

Depositor

Douglas Rees

Article

Nguyen et al Biol Chem. 2015 Sep;396(9-10):1127-34. d

Insert

Methionine Binding Protein (metQ )

Tags

6x Histidine Tag

Expression

Bacterial

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pJL580

Plasmid

#78185

Purpose

Ce rpy-1 expression in worms

Depositor

Junho Lee

Article

Nam et al J Biol Chem. 2009 Mar 20;284(12):8195-20

Insert

rpy-1 (rpy-1 Nematode)

Expression

Worm

Promoter

rpy-1

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pET11a+T214V

Plasmid

#66134

Purpose

Expresses the T214V mutant of CYP119

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Expression

Bacterial

Mutation

T214V

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pJZC103

Plasmid

#62340

Purpose

sgRNA for mammalian cells with mCherry marker

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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SNAP-EF(N)-20nmSAH-GFP(1-10)

Plasmid

#61661

Purpose

20nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-20nmSAH-GFP(1-10)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 S69A

Plasmid

#51590

Purpose

expresses myc-tagged Rem2 S-A mutant

Depositor

Suzanne Paradis

Article

Ghiretti et al J Neurosci. 2013 Apr 10;33(15):6504-15.

Insert

Rem2 (Rem2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Serine 69 to Alanine; also RNAi resistant

Promoter

CMV

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 S308A

Plasmid

#51592

Purpose

expresses myc-tagged Rem2 S-A mutant

Depositor

Suzanne Paradis

Article

Ghiretti et al J Neurosci. 2013 Apr 10;33(15):6504-15.

Insert

Rem2 (Rem2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Serine 308 to Alanine; also RNAi resistant

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Rem2 S241A

Plasmid

#51591

Purpose

expresses myc-tagged Rem2 S-A mutant

Depositor

Suzanne Paradis

Article

Ghiretti et al J Neurosci. 2013 Apr 10;33(15):6504-15.

Insert

Rem2 (Rem2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Serine 241 to Alanine; also RNAi resistant

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSS63

Plasmid

#47951

Purpose

rtTA positive feedback loop with single tet operator in the GAL1 promoter

Depositor

Attila Becskei

Article

Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

Insert

rtTA

Use

Tet inducible expression

Expression

Yeast

Promoter

T3

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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PCx35-pcDNA-S110A

Plasmid

#42917

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35

Expression

Mammalian

Mutation

Ser110 to Ala

Promoter

CMV

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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PCx35CT-S298ter

Plasmid

#42913

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35 C-terminal domain

Tags

6x His, GST, and S-tag

Expression

Bacterial

Mutation

Ser298 stop codon

Promoter

T7

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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PCx35-pcDNA-S298ter

Plasmid

#42925

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35

Expression

Mammalian

Mutation

Ser298 to stop codon

Promoter

CMV

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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PCx35CT-S276A

Plasmid

#42912

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35 C-terminal domain

Tags

6x His, GST, and S-tag

Expression

Bacterial

Mutation

Ser276 to Ala

Promoter

T7

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-2T Crkl SH2SH3

Plasmid

#36404

Depositor

Nora Heisterkamp

Article

de Jong et al J Biol Chem. 1995 Sep 15;270(37):21468-7

Insert

Crkl SH2 and N-terminal SH3 domains (CRKL Human)

Tags

GST

Expression

Bacterial

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
pSUPER.retro.puro-THAP11-A

Plasmid

#36993

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

THAP11 shRNA (THAP11 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 15, 2012

Availability

Academic Institutions and Nonprofits only
pSUPER.retro.puro-THAP11-C

Plasmid

#36994

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

THAP11 shRNA (THAP11 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 15, 2012

Availability

Academic Institutions and Nonprofits only
pSUPER.retro.puro-THAP11-E

Plasmid

#36995

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

THAP11 shRNA (THAP11 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 15, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-L68A

Plasmid

#33059

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

L68A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-D64A

Plasmid

#33060

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

D64A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-R58A

Plasmid

#33061

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

R58A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-F96A

Plasmid

#33063

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

F96A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-F95A

Plasmid

#33066

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

F95A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pGMEH-P38-luxAFB-DAS+4

Plasmid

#27064

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxA-FLAG-luxB-DAS+4

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pGMCtN-P38-luxAB-DAS+4

Plasmid

#27067

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxAB-DAS+4 integrative

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pGEX2TK E7 del EDE

Plasmid

#13675

Depositor

Karl Munger

Article

Jones et al Genes Dev. 1997 Aug 15. 11(16):2101-11.

Insert

HPV 16 E7 del EDE (E7 Human)

Tags

GST

Expression

Bacterial

Mutation

del aa 35-37

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pLX_311-KRAB-dCas9

Plasmid

#96918

Purpose

for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

Depositor

John Doench, William Hahn, David Root

Article

Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

Insert

dCas9

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

dCas9

Promoter

EF1a

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA-BSD

Plasmid

#175583

Purpose

Expresses sgRNA for CasRx in mammalian cell

Depositor

Qi Xie

Article

Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-MBP-TEV

Plasmid

#69929

Purpose

bacterial expression of MBP-TEV n-terminal fusions

Depositor

Zita Balklava, Thomas Wassmer

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

MBP and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Jan. 8, 2016

Availability

Academic Institutions and Nonprofits only

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Cys-3HB-SPYcatcher

Plasmid

#214838

Purpose

This plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

Cys-3HB-SPYcatcher

Tags

His-TEV-SUMO-

Expression

Bacterial

Promoter

T5

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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