We narrowed to 11,246 results for: SHA

1. pEJS436-pCSDest2-AcrIIC2Nme

   Plasmid

   #85712

   Purpose

   Mammalian expression of Type II-C anti-CRISPR protein AcrIIC2Nme from Neisseria meningitidis

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Insert

   Type II-C anti-CRISPR AcrIIC2Nme
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Clover-Nup50-N-10

   Plasmid

   #56303

   Purpose

   Localization: Nuclear Pore, Excitation: 505, Emission: 515

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Nup50 (NUP50 Human)
   

   Use

   Tags

   Clover

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 18, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pcDNA-HA-hTRAF3 88-C

   Plasmid

   #44034

   Depositor

   Shao-Cong Sun

   Article

   Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

   Insert

   TNF receptor-associated factor 3 (TRAF3 Human)
   

   Use

   Tags

   1xHA

   Expression

   Mammalian

   Mutation

   Contains amino acids 89-568; V274D and S393G

   Promoter

   Available since

   March 27, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pUAST mito roGFP2-Orp1

   Plasmid

   #64997

   Purpose

   for expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophila

   Depositor

   Tobias Dick

   Article

   Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Tags

   roGFP2

   Expression

   Insect

   Mutation

   mitochondrial targeting sequence

   Promoter

   Available since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. R10-3 /pQE-30

   Plasmid

   #79859

   Purpose

   The first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore.

   Depositor

   Konstantin Lukyanov

   Article

   Mishin et al Biochemistry. 2008 Apr 22;47(16):4666-73

   Insert

   R10-3
   

   Use

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   Aequorea victoria green fluorescent protein (avGF…

   Promoter

   T5 promoter / lac operator

   Available since

   Sept. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC1300_pUB10_pco-nCASphi_E9t_V2_U6_AtPDS3_gRNA10

   Plasmid

   #197965

   Purpose

   T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pco-nCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26 and pUBQ10

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEJS481-pCDest2-AcrE2-mTagBFP2-IRES

   Plasmid

   #85749

   Purpose

   Expresses anti-CRISPR protein from Type I-E and mTagBFP2

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Inserts

   Type I-E anti-CRISPR
   mTagBFP2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. A3H HapII-Cas9n-UGI

   Plasmid

   #119141

   Purpose

   Base editor made from APOBEC3H Haplotype II

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3H Haplotype II-Cas9 nickase-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   None

   Promoter

   CMV

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4-Claspin-Flag

   Plasmid

   #136246

   Purpose

   Protein expression of Claspin with a C terminal FLAG tag

   Depositor

   Aziz Sancar

   Article

   Sar et al J Biol Chem. 2004 Sep 17;279(38):39289-9

   Insert

   Claspin
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   E263G, Q499R, I783T, D932G and S1062G- Please see…

   Promoter

   Available since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TIA1 RRM-CFP-FRBx5

    Plasmid

    #103780

    Purpose

    expresses iPOLYMER component in mammalian cells

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    TIA1 RRM-CFP-FRBx5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK233 (AAVS1 CMV-AtAFB2)

    Plasmid

    #140537

    Purpose

    AAVS1 CMV-AtAFB2

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-AtAFB2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLN431 (FuGW-G8p-mKate2-1Pe )

    Plasmid

    #105186

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-mKate2-1Pe
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. 874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

    Plasmid

    #159671

    Purpose

    Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    ExuL-NLS-hSpCas9-T2A-GFP
    

    Use

    Tags

    T2A-GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAimR

    Plasmid

    #98687

    Purpose

    Expresses AimR gene from the B. subtilis phage phi3T, with its native promoter, C' His-tag

    Depositor

    Rotem Sorek

    Article

    Erez et al Nature. 2017 Jan 26;541(7638):488-493. d

    Insert

    AimR
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Native

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-GW-V5-Hygro

    Plasmid

    #68265

    Purpose

    Constitutive selectable expression of C-term V5 tagged proteins

    Depositor

    Mitchell Guttman

    Article

    McHugh et al Nature. 2015 May 14;521(7551):232-6. doi

    Type

    Empty backbone

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

16. Clover-Zyxin-6

    Plasmid

    #56531

    Purpose

    Localization: Focal Adhesions, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Zyxin (ZYX Human)
    

    Use

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. IBMc097

    Plasmid

    #161945

    Purpose

    Contains Type IIS restriction site free elements for IBM staging vectors. Staging plasmid for IBM on mCherry

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1C3(BsmBI-)-BsaI-mCherry*(27-218)-BsaI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3xFLAG-CMV-zDicer

    Plasmid

    #171539

    Purpose

    Encodes Zebrafish Dicer protein

    Depositor

    Yan Zeng

    Article

    Li et al Biochem Biophys Res Commun. 2019 Jun 18;

    Insert

    dicer1(MK642699) (dicer1 Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-h-mmPtch1-B

    Plasmid

    #120888

    Purpose

    Expresses Ptch1-B for function assays

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLN410 (FuGW-S(E2F1)p-ECFP sponge control)

    Plasmid

    #105182

    Purpose

    Module 2 control - synthetic promoter E2F1 drives ECFP only (no miR1 binding sites)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(E2F1)p-ECFP (Cerulean) sponge control
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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