We narrowed to 13,291 results for: cDNA

1. pCDNA3.1-HsPcdh21-Myc-Flag-His

   Plasmid

   #210818

   Purpose

   Mammalian overexpression vector for C-terminally Myc and Flag and His tagged human Pchd21 (WT)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Protocadherin 21 (CDHR1 Human)
   

   Use

   Tags

   Myc, 3x Flag, 10x His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3 Flag p38 beta2 (agf)

   Plasmid

   #20356

   Depositor

   Roger Davis

   Article

   Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

   Insert

   pDCNA3 Flag p38 beta(agf) (MAPK11 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Replace Thr 182 and Tyr 184 with Ala and Phe resp…

   Promoter

   Available Since

   May 11, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3-Venus-cpVenus-FLARE-AKAR(T/A)

   Plasmid

   #123330

   Purpose

   Negative control mutant for Venus-cpVenus-FLARE-AKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-AKAR(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3.1-Flag-H2A K13-15R

   Plasmid

   #63563

   Purpose

   Expression of human H2A with K13R and K15R tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysine 13 and Lysine 15 are now Arginine

   Promoter

   CMV

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCDNA5/FRT/TO_COX8-Halo-PanK

   Plasmid

   #197949

   Purpose

   Expression of FRET_control in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

   Insert

   Halo-ecPanK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PanK=PanK-D127A,L277W,F252Y

   Promoter

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon

   Plasmid

   #69906

   Purpose

   Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ciRS7 (CDR1-AS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C58

   Plasmid

   #171428

   Purpose

   Expression of FKBP-(GGS)X6-HaloTag C58 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   FKBP-(GGS)X6-HaloTag C58
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Lyn-dPlcR(K30/32L)

   Plasmid

   #181833

   Purpose

   Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid.

   Depositor

   Jin Zhang

   Article

   Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

   Insert

   Lyn-dPlcR (K30L,K32L)
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase, dd…

   Expression

   Mammalian

   Mutation

   Lysines 30 and 32 in PH domain mutated to leucine.

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO-Venus-Flag (865)

   Plasmid

   #40998

   Depositor

   Jonathon Pines

   Article

   Sedgwick et al EMBO J. 2013 Jan 23;32(2):303-14. doi: 1

   Type

   Empty backbone

   Use

   Tags

   3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-Venus-Venus-FLARE-Cameleon

    Plasmid

    #123351

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-Venus-FLARE-Cameleon
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-GFP-LpIA(wild-type)

    Plasmid

    #61821

    Purpose

    Encodes a wild-type sequence of LplA and serves as the negative control for resorufin labeling

    Depositor

    Alice Ting

    Article

    Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

    Insert

    E. coli lipoic acid ligase
    

    Use

    Tags

    6XHis, EGFP, and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA5/FRT/TO_CoA-Snifit(V97T)

    Plasmid

    #197944

    Purpose

    Expression of CoA-Snifit in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

    Insert

    Halo-GFP-ecPanK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PanK=PanK-V97T, D127A,L277W,F252Y

    Promoter

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA5/FRT/TO_CoA-Snifit(G41)

    Plasmid

    #197942

    Purpose

    Expression of CoA-Snifit in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

    Insert

    Halo-GFP-ecPanK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PanK=PanK-D127A,L277W,F252Y

    Promoter

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-Lyso-mCherry-Rheb(C181S)

    Plasmid

    #192438

    Purpose

    Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    Lyso-mCherry-Rheb(C181S) (RHEB Human)
    

    Use

    Tags

    Full-length LAMP1 and mCherry

    Expression

    Mammalian

    Mutation

    Rheb Cys 181 mutated to Ser; abolishes C-terminal…

    Promoter

    CMV

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3-Myc3-Cul3 delta N41

    Plasmid

    #21590

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

    Insert

    cullin 3 (CUL3 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Residues 34-74 deleted from N terminus.

    Promoter

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-3myc-6His-EZH2 21D

    Plasmid

    #42664

    Depositor

    Mien-Chie Hung

    Article

    Cha et al Science. 2005 Oct 14;310(5746):306-10.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    3Myc and 6His

    Expression

    Mammalian

    Mutation

    S21D

    Promoter

    Available Since

    July 17, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.2/DEST/hCx43-E2HA-stop

    Plasmid

    #40909

    Depositor

    Robin Shaw

    Article

    Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

    Insert

    Connexin 43 internal HA tag (GJA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HA tag inserted in second extracellular loop

    Promoter

    CMV

    Available Since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-3myc-6His-EZH2 21A

    Plasmid

    #42663

    Depositor

    Mien-Chie Hung

    Article

    Cha et al Science. 2005 Oct 14;310(5746):306-10.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    3Myc and 6His

    Expression

    Mammalian

    Mutation

    S21A

    Promoter

    Available Since

    July 17, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA5-FRT-GFP-mCherry-3pGW

    Plasmid

    #53965

    Purpose

    Bidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherry

    Depositor

    Saeed Tavazoie

    Article

    Oikonomou et al Cell Rep. 2014 Apr 10;7(1):281-92. doi:

    Inserts

    GFP
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-Flag mTOR S2035T D2357E

    Plasmid

    #26605

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S2035T D2357E

    Promoter

    Available Since

    Feb. 1, 2011

    Availability

    Academic Institutions and Nonprofits only