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eGFPbait-E2A-KalTA4-pA donor vector

Plasmid

#61069

Purpose

for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)

Depositor

Filippo Del Bene

Publication

Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

Inserts

eGFPbait
KalTA4

Use

Zebrafish expression

Tags

E2A

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CAG-Flex-TC66T

Plasmid

#48331

Purpose

Cre-dependant TVA receptor-mCherry fusion with a mutation (66T) in TVA

Depositor

Liqun Luo

Publication

Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

Insert

TVA receptor mCherry fusion with the 66T mutation

Use

AAV and Cre/Lox

Tags

mCherry fusion to TVA receptor

Expression

Mammalian

Mutation

66T

Promoter

CAG

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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hRIP1 HA GFP wt

Plasmid

#41396

Depositor

Francis Chan

Publication

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP1 (RIPK1 Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

E96G

Promoter

CMV

Available since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

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PKC gamma WT

Plasmid

#21236

Depositor

Bernard Weinstein

Publication

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC gamma (Prkcg Mouse)

Tags

HA

Expression

Mammalian

Available since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
FLAG.PKCepsilon.K/W

Plasmid

#10796

Depositor

Alex Toker

Publication

Cenni et al Biochem J. 2002 May 1. 363(Pt 3):537-45.

Insert

PKC epsilon (PRKCE Human)

Tags

FLAG

Expression

Mammalian

Mutation

Kinase dead mutant: K437W (Lys 437 to Trp)

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
1247 pCMVb p300 HA delta33

Plasmid

#10719

Depositor

William Sellers

Insert

p300 delta33 (EP300 Human)

Tags

HA

Expression

Mammalian

Mutation

Mutant does not bind to E1A. Amino acids 1737 to…

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pJQ200SK

Plasmid

#78497

Purpose

Suicide vector for gene replacement

Depositor

Michael Hynes

Publication

Quandt et al Gene. 1993 May 15;127(1):15-21.

Type

Empty backbone

Use

Suicide plasmid

Available since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pU6d:sgRNA#5

Plasmid

#64249

Purpose

expresses sgRNA under U6d promoter

Depositor

Wenbiao Chen

Publication

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pORANGE Tubb3-GFP KI

Plasmid

#131497

Purpose

Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Tubb3, Tubb3 Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBABEpuro mTurquoise2 LC3B

Plasmid

#78518

Purpose

Fluorescent reporter for LC3B visualization

Depositor

David Chan

Publication

Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

Insert

LC3B (MAP1LC3B Human)

Use

Retroviral

Tags

mTurquoise2

Expression

Mammalian

Promoter

retroviral

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-Intersectin Long

Plasmid

#47395

Depositor

Peter McPherson

Publication

Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

Insert

intersectin long (ITSN1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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PH-Akt(R25C)-GFP

Plasmid

#51466

Purpose

Non-PIP3 binding control for PH-Btk-GFP

Depositor

Tamas Balla

Publication

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

AKT (aa 1–164)

Tags

EGFP

Expression

Mammalian

Mutation

R25C

Promoter

CMV

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
hRIP3 GFP RHIM mut

Plasmid

#41385

Depositor

Francis Chan

Publication

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Mutation

RHIM mutant (AAAA-459-462)

Promoter

CMV

Available since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGL3E-hIL-17prom(-1125)-Luc

Plasmid

#25627

Depositor

Sarah Gaffen

Publication

Liu et al J Biol Chem. 2004 Dec 10. 279(50):52762-

Insert

human IL-17 promoter (IL17A Human)

Use

Luciferase

Available since

June 25, 2010

Availability

Academic Institutions and Nonprofits only

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Flag p21 T145A

Plasmid

#16241

Depositor

Mien-Chie Hung

Publication

Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

Insert

p21 (CDKN1A Human)

Tags

Flag

Expression

Mammalian

Mutation

T145A

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pFRT_TO_DESTFLAGHA

Plasmid

#26361

Depositor

Thomas Tuschl

Publication

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Type

Empty backbone

Tags

FLAG/HA

Expression

Mammalian

Available since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
PB-TO-MYOD1-shOct4

Plasmid

#182309

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

MYOD1-shOct4 (MYOD1 Human)
puro-T2A-mycNLS-mTagBFP2

Tags

T2A-mycNLS-mTagBFP2

Expression

Mammalian

Mutation

shOct4

Promoter

EF1a and TRE3G

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Syn-FLEX-rc[Chronos-GFP]

Plasmid

#62722

Purpose

AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

human synapsin promoter

Available since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCS-H2B-cerulean

Plasmid

#53748

Purpose

Contains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.

Depositor

Sean Megason

Publication

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

Cerulean

Expression

Mammalian

Promoter

simian CMV IE94

Available since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mH2A1.1-CT-GFP

Plasmid

#45167

Depositor

Brian Chadwick, Hunt Willard

Publication

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy (MACROH2A1 Human)

Tags

GFP

Expression

Mammalian

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only

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