We narrowed to 10,393 results for: plasmids 101

1. (SIM)5

   Plasmid

   #126947

   Purpose

   Bacterial expression plasmid for (SIM)5 client to assess SUMO-SIM scaffolds.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   5 repeats of SUMO interacting motif (SIM) from PIASx, each separated by (GGS)4 (PIAS2 Human)
   

   Expression

   Bacterial

   Promoter

   unknown

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLC133

   Plasmid

   #198639

   Purpose

   Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

   Depositor

   David Bikard

   Article

   Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

   Insert

   dCas9
   

   Tags

   3x-FLAG

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. anti-Notch2_B6-pBIOCAM5

   Plasmid

   #39347

   Depositor

   John McCafferty

   Article

   Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

   Insert

   scFv-Fc-fusion specific for Notch2 (clone B6) (NOTCH2 Human)
   

   Tags

   3xFLAG, 6xHis, and human Fc

   Expression

   Mammalian

   Available Since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV CAGG eGFP

   Plasmid

   #107707

   Purpose

   express eGFP in variety of cell types

   Depositor

   Troy Margrie

   Article

   Velez-Fort et al Neuron. 2018 Apr 4;98(1):179-191.e6. doi

   Insert

   eGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAGG

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUW-TetO-Nanog

   Plasmid

   #40800

   Depositor

   Rudolf Jaenisch

   Article

   Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

   Insert

   Nanog (Nanog Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Minimal CMV + TetO

   Available Since

   Oct. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

6. AAV8-cPE-N

   Plasmid

   #183538

   Purpose

   Deliver a split compact Prime Editor in vivo

   Depositor

   Wen Xue

   Article

   Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

   Insert

   N-terminal fragment of SpCas9 nickase (H840A)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-(SIM)3

   Plasmid

   #126955

   Purpose

   Bacterial expression plasmid for eGFP-(SIM)3 client to assess SUMO-SIM scaffolds.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   EGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
   

   Tags

   eGFP

   Expression

   Bacterial

   Promoter

   unknown

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_PGK_SNIPR_(GGS)6_SNIPR

   Plasmid

   #188344

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)6, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_(GGS)6_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLENTI CMV GFP TREX1 R128A R174A BLAST

   Plasmid

   #164234

   Purpose

   lentiviral plasmid for GFP-TREX1 R128A R174A

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R124A R174A

   Promoter

   CMV

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-GFAP(long)-FLEX-GCaMP7f

    Plasmid

    #189612

    Purpose

    Cre-dependent GCaMP7f under the GFAP promoter

    Depositor

    Martin Riccomagno

    Article

    Agnew-Svoboda et al Cell Rep Methods. 2022 Aug 22;2(8):10027

    Insert

    GCaMP7f
    

    Use

    AAV and Cre/Lox

    Tags

    GCaMP7f

    Expression

    Mammalian

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDuRCC-N

    Plasmid

    #81194

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please view depositor comments below and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. DIO-EF1a-FLEX-H2B-GFP-P2A-N2c(G)

    Plasmid

    #73476

    Purpose

    Encodes the G protein to enable transsynaptic RAV infection

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    H2B-GFP-P2A-N2c(G)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Gad65P-eGFP-3XNLS-WPRE-pA

    Plasmid

    #183804

    Purpose

    AAV vector to express nuclear localized eGFP from a Gad65 promoter

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

    Insert

    eGFP-NLS
    

    Use

    AAV

    Tags

    NLS

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-(SIM)1

    Plasmid

    #126954

    Purpose

    Bacterial expression plasmid for eGFP-(SIM)1 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    EGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
    

    Tags

    eGFP

    Expression

    Bacterial

    Promoter

    unknown

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-Cas13d-T2A-GFP(1-10)

    Plasmid

    #224566

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    Cas13d-T2A-GFP(1-10)
    

    Use

    CRISPR

    Tags

    T2A-split GFP(1-10)

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. gh171

    Plasmid

    #106832

    Purpose

    expression of gRNA targeting UGCG

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    UGCG (UGCG Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIC306

    Plasmid

    #52257

    Purpose

    Retroviral expression of EGFP-TEV-S-tag SKA3 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Article

    Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

    Insert

    Ska3 (SKA3 Human)
    

    Use

    Retroviral

    Tags

    EGFP, S tag, and TEV protease site

    Expression

    Mammalian

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)/CUG-nLuc-3XFLAG

    Plasmid

    #127300

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)/GUG-nLuc-3XFLAG

    Plasmid

    #127301

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-hSyn-Gβγ-BERKY1

    Plasmid

    #158429

    Purpose

    BRET biosensor for detection of free Gβγ in lentiviral vector pLenti-hSyn

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-YFP-GRK3ct
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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