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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,228 results for: gateway

Showing: 8851 - 8880 of 18228 results

  1. His8:MBP-tev-Arg-eΔK-Flv

    Plasmid
    #83396
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83396-plasmid-map-sequence-id-175052

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  2. His8:MBP-tev-Phe-eΔK-Flv

    Plasmid
    #83395
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83395-plasmid-map-sequence-id-175037

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  3. pVP16-tev-PRT1-C195A

    Plasmid
    #83408
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    C195A substitution
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83408-plasmid-map-sequence-id-175084

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  4. pVP16-tev-PRT1-C29A

    Plasmid
    #83407
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    C29A substitution
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83407-plasmid-map-sequence-id-175083

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  5. pVP16-tev-PRT1-V28A

    Plasmid
    #83406
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    V28A substitution
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83406-plasmid-map-sequence-id-175082

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  7. psicheck_ETF1_3UTR

    Plasmid
    #65884
    Purpose
    Luciferase assay vector, assessing regulation of ETF1 3' UTR
    Depositor
    Thomas Tuschl
    Insert
    ETF1 (ETF1 Human)
    Expression
    Mammalian
    Available Since
    July 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pUASp YFP Rac2 CA

    Plasmid
    #52238
    Purpose
    Drosophila expression of YFP-Rac2 constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Rac2 (Rac2 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q61L; CA mutation
    Promoter
    GAL4
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pUASp YFP Rac2 DN

    Plasmid
    #52237
    Purpose
    Drosophila expression of YFP-Rac2 dominant negative mutant
    Depositor
    Matthew Scott
    Insert
    Rac2 (Rac2 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    T17N; DN mutation
    Promoter
    GAL4
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. IDG_CNGA4_OE_1

    Plasmid
    #161675
    Purpose
    Overexpresses CNGA4
    Depositor
    Mike McManus
    Insert
    CNGA4 (CNGA4 Human)
    Tags
    V5
    Expression
    Mammalian
    Availability
    Academic Institutions and Nonprofits only
    161675-plasmid-map-sequence-id-319726

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  11. IDG_CHRND_OE_1

    Plasmid
    #161688
    Purpose
    Overexpresses CHRND
    Depositor
    Mike McManus
    Insert
    CHRND (CHRND Human)
    Tags
    V5
    Expression
    Mammalian
    Availability
    Academic Institutions and Nonprofits only
    161688-plasmid-map-sequence-id-334568

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  15. pSLIK_hygro_3xflag_NRF2(RR)

    Plasmid
    #136535
    Purpose
    Lentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)
    Depositor
    Kevin Janes
    Article
    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13
    Insert
    NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)
    Use
    Lentiviral; Destinatioin vector for gateway cloni…
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    This plasmid is developed by mutating 3 base pair…
    Available Since
    July 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    136535-plasmid-map-sequence-id-304676

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  19. pTripz NEO DEST

    Plasmid
    #209072
    Purpose
    Modified DOX-ON inducible destination vector with a c-terminal 3X flag tag to be used for LR recombination reactions to inducibly express cDNAs of interest
    Depositor
    Matthew Oser
    Article
    Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector to be used…
    Tags
    3X FLAG
    Promoter
    CMV
    Available Since
    Nov. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    209072-plasmid-map-sequence-id-412301

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Showing: 8851 - 8880 of 18228 results