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pcDNA3.2 mir-1-1 reporter dme-mir-2a-1

Plasmid

#46664

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

dme-mir-2a-1

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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ZE42 myc-Dapper-1 pCS2P+

Plasmid

#17083

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

Dapper-1 (dact1 Zebrafish)

Use

Zebrafish expression

Tags

myc

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pTTSTOP

Plasmid

#41751

Depositor

Jeff Gelles

Article

Thoresen et al Biochemistry. 2008 Sep 9;47(36):9514-21.

Insert

kinesin heavy chain (Khc Fly)

Expression

Bacterial

Mutation

residues 1-401

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
XE243 Mouse CK2beta CS2p+

Plasmid

#16729

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

Casein Kinase II beta-pCS2P+ (Csnk2b Mouse)

Use

Xenopus expression

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
Khc73(1-547)-GFP

Plasmid

#31749

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Khc73 (Khc-73 Fly)

Tags

GFP

Expression

Mammalian

Mutation

Contains only aa 1-547

Promoter

Metallothionein

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only
pFastBac SOX R139A

Plasmid

#192058

Purpose

Expression vector for SOX (ORF37) R139A

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37)

Expression

Insect

Mutation

R139A

Promoter

Polyhedrin

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac SOX Q129H

Plasmid

#192057

Purpose

Expression vector for SOX (ORF37) Q129H

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37) Q129H

Expression

Insect

Mutation

Q129H

Promoter

Polyhedrin

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM227

Plasmid

#226650

Purpose

MSS-His6-UFD1L

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

UFD1 (UFD1 Human)

Tags

His-thrombin

Expression

Bacterial

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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WNV NS2B-NS3 protease (catalytically active, self cleave); aliases: West Nile Virus NS2B-NS3 fusion protein

Plasmid

#204795

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B-NS3 fusion

Expression

Bacterial

Available Since

Aug. 29, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-TRBP-Y358A

Plasmid

#215890

Purpose

Expression of TRBP-Y358A

Depositor

Kumiko Ui-Tei

Article

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

TRBP-Y358A (TARBP2 Human)

Expression

Mammalian

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TRBP-L326A

Plasmid

#215889

Purpose

Expression of TRBP-L326A

Depositor

Kumiko Ui-Tei

Article

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

TRBP-L326A (TARBP2 Human)

Expression

Mammalian

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pB-Neo-mCherry-mDYNLL1

Plasmid

#210336

Purpose

Overexpression of mDYNLL1

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

mDYNLL1 (Dynll1 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Sars Cov 2 MPRO R188S mutantSARS-CoV-2 Mpro protease (R188S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204785

Purpose

Bacterial expression of Sars Cov 2 MPRO R188S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

R188S

Available Since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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ZIKV NS2B-NS3 protease (H115A- catalytically inactive); aliases: Zika Virus NS2B-NS3 fusion protein expressed with N-terminal Tw

Plasmid

#204791

Purpose

Bacterial expression of a fusion of Zika NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

Zika NS2B-NS3 fusion

Tags

6 His

Expression

Bacterial

Available Since

Jan. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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WNV NS2B-NS3 protease (based on PDB 2IJO, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion protein

Plasmid

#204799

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B-NS3 fusion

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (R188K mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204770

Purpose

Bacterial expression of Sars Cov 2 MPRO R188K mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

R188K col1

Available Since

Nov. 30, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-WDR5-P2A-SH2-P2A-Ras-CAAX

Plasmid

#209712

Purpose

Mammailan expression of WDR5, SH2, and HRas separated by P2A.

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

WDR5-P2A-SH2-P2A-Ras-CAAX

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-NLS-MBP-rATOM(WDR5)

Plasmid

#209710

Purpose

Mammalian expression of r-ATOM biosensor for WDR5 with nuclear localization signal

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

NLS-MBP-rATOM(WDR5)

Tags

NLS-MBP

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1_dAP1-2

Plasmid

#203837

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1 with both AP-1 sites knocked out

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dAP1-2

Expression

Mammalian

Mutation

KO of both AP1 sites

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCX:cIPAD-3 (ΔICD)

Plasmid

#208518

Purpose

ddGFP-A and ddGFP-B3-inserted Pcdhα4 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al iScience. 2023 Jul 17;26(7):107238. doi:

Insert

Pcdha4 (Pcdha4 Mouse)

Tags

HA tag

Expression

Mammalian

Promoter

Chicken β-actin

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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