We narrowed to 9,384 results for: Coli

1. pMH-HT_RcCHAD

   Plasmid

   #127782

   Purpose

   Expresses RcCHAD in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   RcCHAD
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAtLCYbSK

   Plasmid

   #53588

   Purpose

   Produces an active Arabidopsis thaliana lycopene beta-cyclase enzyme in E. coli. Also known as pAt9SK.

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

   Insert

   lcyB (LYC Mustard Weed)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. MS2-ecDHFR

   Plasmid

   #65859

   Purpose

   Expresses RNA-binding protein MS2 with eDHFR fusion tag

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

   Inserts

   MS2
   E. coli Dihydrofolate Reductase (folA )
   

   Use

   Tags

   HA and NLS

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCDW115

   Plasmid

   #70062

   Purpose

   artificial transcription template "pause ablation"

   Depositor

   Jeff Gelles

   Article

   Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

   Insert

   lambda PR' A+2G T+6G - repeat cassette - E. coli rpoB - lambda TR'
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pGEX-4T1 mouse Tankyrase1 5ARC (171–961)

   Plasmid

   #132638

   Purpose

   expresses mouse Tankyrase1 5ARC (171–961) in E. coli

   Depositor

   Wenqing Xu

   Article

   DaRosa et al Protein Sci. 2018 Jun;27(6):1057-1067. d

   Insert

   Tankyrase1 (171–961)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSETA_crE6

   Plasmid

   #172928

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 1 helix and 2 GSG linkers

   Depositor

   Arnold Boersma

   Article

   Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

   Insert

   CrowdingSensorE6
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-P70a-Bottom-T

   Plasmid

   #87313

   Purpose

   Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli system "Bottom" transcript
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P70a

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTH07

   Plasmid

   #70063

   Purpose

   artificial transcription template "distal pause"

   Depositor

   Jeff Gelles

   Article

   Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

   Insert

   lambda PR' - repeat cassette - E. coli rpoB with inserted pause - lambda TR'
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

9. 36INS-J23100_GB

   Plasmid

   #78669

   Purpose

   36INS-J23100_GB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [G:36INS-J23100:B]

   Depositor

   Douglas Densmore

   Article

   Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

   Insert

   Insulated J23100 Promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 36INS-J23100_FB

    Plasmid

    #78668

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [F:36INS-J23100:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated J23100 Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 36INS-J23100_EB

    Plasmid

    #78667

    Purpose

    36INS-J23100_EB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [E:36INS-J23100:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated J23100 Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 36INS-J23100_AB

    Plasmid

    #78666

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_J23100. [A:36INS-J23100:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated J23100 Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHBFD1

    Plasmid

    #53565

    Purpose

    Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

    Insert

    hbfd1 cDNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRL1058

    Plasmid

    #70686

    Purpose

    Tn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.

    Depositor

    Peter Wolk

    Article

    Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88

    Insert

    Transposon Tn5-1058
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-28a-CylA

    Plasmid

    #48198

    Purpose

    Expresses CylA (fatty acyl-AMP ligase) in E. coli

    Depositor

    Emily Balskus

    Article

    Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

    Insert

    CylA
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. T7-AglC-His6

    Plasmid

    #89726

    Purpose

    Expresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

    Depositor

    Barbara Imperiali

    Article

    Zamora et al Methods Enzymol. 2017;597:145-186. doi:

    Insert

    AglC
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. T7-AglK-His

    Plasmid

    #89714

    Purpose

    Expresses AglK from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

    Depositor

    Barbara Imperiali

    Article

    Zamora et al Methods Enzymol. 2017;597:145-186. doi:

    Insert

    AglK
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC19-P70a-Top-T

    Plasmid

    #87312

    Purpose

    Expresses the "Top" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system "Top" transcript
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p70rg 1

    Plasmid

    #17828

    Depositor

    Jay Keasling

    Article

    Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

    Insert

    E. coli optimized red flourescent protein (RFPec), GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pET28-p53 (Dog 1-77)

    Plasmid

    #62060

    Purpose

    Expression of Dog p53 (residues 1-77) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-77

    Promoter

    T7

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only