We narrowed to 11,977 results for: pcDNA

1. pcDNA3.1 FLAG-SIV-RCM-vpx

   Plasmid

   #115844

   Purpose

   Encodes codon optimized FLAG tagged SIVRCM-Vpx

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   SIVRCM-Vpx
   

   Use

   Tags

   3x-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3puro-mGFP-ROCK2-C (941-1388)

   Plasmid

   #225471

   Purpose

   Express mEGFP-fusion protein of ROCK2 fragment (941-1388)

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   ROCK2 (ROCK2 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   C terminal fragment (941-1388)

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF

   Plasmid

   #201217

   Purpose

   pCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTF

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   Flag and SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNATM3.1/V5-His TOPO-PKCi-K274W

   Plasmid

   #55690

   Purpose

   expresses dominant negative human PKCi tagged with V5

   Depositor

   Pedro Salas

   Article

   Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

   Insert

   Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   A120E and K274W

   Promoter

   CMV

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1-CD81-(TEV)-FKBP-Myc

   Plasmid

   #177908

   Purpose

   human CD81-TEV cleavage site-FKBP-Myc tag

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

   Insert

   human CD81 (CD81 Human, Synthetic)
   

   Use

   Tags

   FKBP, Myc tag, and TEV protease cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 Flag MFF iso2 S172A

   Plasmid

   #74392

   Purpose

   Flag tagged human S172A MFF isoform 2 mutant

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser 172 Ala (numbering based on MFF isoform 1)

   Promoter

   Available since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-Flag-RNF168 delta RING

   Plasmid

   #133982

   Purpose

   mammalian expression vector of Flag tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deletion of RING

   Promoter

   CMV

   Available since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. YFP(1-158)-beta-1 in pcDNAI/Amp

   Plasmid

   #54468

   Purpose

   An amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

   Insert

   YFP (1-158)/beta-1 (GNB1 Aequorea victoria, Human)
   

   Use

   Tags

   YFP(1-158) was fused to the amino terminus of Gbe…

   Expression

   Mammalian

   Mutation

   GBeta-1 was amplified via PCR, which removed an i…

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 LIC 6A FLAG-TEV-DPP8

   Plasmid

   #166795

   Purpose

   Mammalian cell expression vector for FLAG-tagged DPP8

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP8 (DPP8 Human)
   

   Use

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Nano-lantern(cAMP-3.3)/pcDNA3

    Plasmid

    #53595

    Purpose

    Nano-lantern-based luminescent cAMP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern-based luminescent cAMP indicator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-GFP-IMP2-2_antisense-control

    Plasmid

    #42174

    Depositor

    Alexandra Kiemer

    Article

    Kessler et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Insulin-like growth factor 2 mRNA binding protein 2-2 antisense control (IGF2BP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

12. Nano-lantern(Ca2+)_150/pcDNA3

    Plasmid

    #51984

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=150nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM-2G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3 mClover LIC cloning vector (6G)

    Plasmid

    #86964

    Purpose

    Empty LIC vector; adds an mClover gene to the C-terminus of your protein of interest.

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Nano-lantern(Ca2+)_11/pcDNA3

    Plasmid

    #51987

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=11nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM-4GS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaPH

    Plasmid

    #22930

    Purpose

    Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PH domain

    Depositor

    Alexandra Newton

    Article

    Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

    Insert

    PHLPP1-deltaPH (PHLPP1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes 127-1205 aa. Deleted amino-terminal PH d…

    Promoter

    CMV

    Available since

    Feb. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

16. Nano-lantern(cAMP-0.4)/pcDNA3

    Plasmid

    #53593

    Purpose

    Nano-lantern-based luminescent cAMP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern-based luminescent cAMP indicator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNATM3.1/V5-His TOPO-PKCi-A120E

    Plasmid

    #55687

    Purpose

    expresses constitutively active human PKCi tagged with V5

    Depositor

    Pedro Salas

    Article

    Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

    Insert

    Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    A120E

    Promoter

    CMV

    Available since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

18. p5072 pcDNA4c hBrd4 (1224-1362)

    Plasmid

    #14451

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 (1224-1362) (BRD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    amino acids 1224-1362

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC

    Plasmid

    #22931

    Purpose

    Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C)

    Depositor

    Alexandra Newton

    Article

    Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

    Insert

    PHLPP1-deltaC (PHLPP1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PHLPP-deltaC, deletion of PDZ binding domain--the…

    Promoter

    CMV

    Available since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

20. 1354 pcDNA3 flag FKHR deltaDB

    Plasmid

    #10694

    Depositor

    William Sellers

    Insert

    FKHR deltaDB (FOXO1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    deletion of amino acids 208-220

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only