We narrowed to 23,940 results for: nes

1. pSCL.95

   Plasmid

   #184986

   Purpose

   Express Cas9/-Eco1 RT fusion, using linker2

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Cas9-fusion_linker2-Eco1RT
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSCL.102

   Plasmid

   #184987

   Purpose

   Express -Eco1 RT-P2A-Cas9

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-P2A-Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSCL.94

   Plasmid

   #184985

   Purpose

   Express -Eco1 RT/Cas9 fusion, using linker2

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-fusion_linker2-Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Hygro-Bem1-mCherry-AID

   Plasmid

   #173925

   Purpose

   Plasmid designed for the genomic tagging of saccharomyces cerevisiae Bem1 with mCherry-AID

   Depositor

   Liedwij Laan

   Article

   Daalman et al Philos Trans R Soc Lond B Biol Sci. 2023

   Inserts

   Partial Bem1 C-terminus
   hygromycin marker
   Bem1 downstream sequence
   

   Use

   Tags

   mCherry- AID

   Expression

   Mutation

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSCL.005

   Plasmid

   #184971

   Purpose

   Express Cas9 in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Integrating inducible cassette: Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   Promoter

   Gal1-10

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSCL.71

   Plasmid

   #184983

   Purpose

   Express -Eco1 RT/Cas9 fusion, using linker1

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-fusion_linker1-Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pnYC-NpM-HsSMG7opt_732-1091_V

   Plasmid

   #147769

   Purpose

   Bacterial Expression of HsSMG7opt_732-1091

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsSMG7opt_732-1091 (SMG7 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. double_sgRNA targeting e1 and e4

   Plasmid

   #190688

   Purpose

   sgRNAs targeting enhancer 1 and 4 of MYC separately

   Depositor

   Stanley Qi

   Article

   Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

   Insert

   sgRNAs targeting enhancer 1 and 4 of MYC separately
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   BFP and Puromycin

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-BLZinCh-P20

   Plasmid

   #188055

   Purpose

   Expression of BLZinCh-P20, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

   Depositor

   Maarten Merkx

   Article

   Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

   Insert

   BLZinCh-P20
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promotor

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-BLZinCh-P30

    Plasmid

    #188056

    Purpose

    Expression of BLZinCh-P30, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

    Depositor

    Maarten Merkx

    Article

    Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

    Insert

    BLZinCh-P30
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. VAMP8

    Plasmid

    #186689

    Purpose

    Expresses VAMP8 in E. Coli

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    VAMP8
    

    Use

    Tags

    3xFLAG, GST, TEV, and Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMLS538

    Plasmid

    #188881

    Purpose

    Gateway Entry Vector [1-2]: 2xNLS::Cas9 (with 4 introns from smu-2)

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Cas9
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    C. elegans codom optimization and intron addition

    Promoter

    Available since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-ammB3

    Plasmid

    #167815

    Purpose

    Expresses His6-tagged AmmB3 in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

    Insert

    AmmB3
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-ammC1

    Plasmid

    #167816

    Purpose

    Expresses His6-tagged AmmC1 in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

    Insert

    AmmC1
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Stx3

    Plasmid

    #186685

    Purpose

    Expresses Syntaxin 3 in E. Coli

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    Syntaxin 3
    

    Use

    Tags

    6 his tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNCS-Clover4-LUMABS-HA

    Plasmid

    #183486

    Purpose

    To express the Clover4-LUMABS-HA sensor for Avian Influenza Virus antibody detection.

    Depositor

    Zongwen Jin, Jun Chu

    Article

    Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

    Insert

    Clover4-LUMABS-HA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNCS-Clover4-LUMABS-DEN1

    Plasmid

    #183487

    Purpose

    To express the Clover4-LUMABS-DEN1 sensor for Dengue Virus antibody dection.

    Depositor

    Zongwen Jin, Jun Chu

    Article

    Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

    Insert

    Clover4-LUMABS-DEN1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KBB394

    Plasmid

    #185086

    Purpose

    NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Nma111 KKR 9-11 AAA; KRK 28-30 AAA

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pgTS40

    Plasmid

    #169630

    Purpose

    pX330 derived vector for PCAG driven expression of SpCas9 and PU6 driven expression of guide RNA OGTS40 (5' GGGGCCACTAGGGACAGGAT 3') targeting position 55115755 of chromosome 19.

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    U6-driven gRNA expression and PCAG-driven SpCas9 expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 / PCAG

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2-foxd4l1.1-F>E

    Plasmid

    #185508

    Purpose

    Expression of Xenopus laevis foxd4

    Depositor

    Sally Moody

    Article

    Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

    Insert

    foxd4l1.1
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    F>E

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti_CMV:Flag-CeNL_SV40:PuroR

    Plasmid

    #183039

    Purpose

    Expresses the FLAG-tagged CeNL (Blue Nanolantern) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    CeNL Nanolantern
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti_CMV:Flag-YeNL_SV40:PuroR

    Plasmid

    #183041

    Purpose

    Expresses the FLAG-tagged YeNL (Yellow Nanolantern) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    YeNL Nanolantern
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti_CMV:Flag-OeNL_SV40:PuroR

    Plasmid

    #183042

    Purpose

    Expresses the FLAG-tagged OeNL (Orange Nanolantern) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    OeNL Nanolantern
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCS2-foxd4l1.1-A6

    Plasmid

    #185506

    Purpose

    Expression of Xenopus laevis foxd4

    Depositor

    Sally Moody

    Article

    Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

    Insert

    foxd4l1.1
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    FSIENIM to AAAAAAM

    Promoter

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJEP209-Lenti-1.3CaMKII P-Intron-Flag-GluN2A-WPRE-pA

    Plasmid

    #74714

    Purpose

    pLenti vector backbone designed to express Flag-GluN2A from a 1.3CaMKII promoter. Transgene in reverse orientation.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Flag-GluN2A
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    1.3CaMKIIa

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEUK028

    Plasmid

    #180826

    Purpose

    T7-inducible expression construct to produce SwGdmA (SWIT_RS16490) in E. coli

    Depositor

    Gregg Beckham

    Article

    Bleem et al Chem Catalysis, 2022

    Insert

    SwGdmA (SWIT_RS16490 Synthetic, Sphingomonas wittichii RW1)
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEUK037

    Plasmid

    #180829

    Purpose

    T7-inducible expression construct to produce CnGdmB (CNE_RS35790) in E. coli

    Depositor

    Gregg Beckham

    Article

    Bleem et al Chem Catalysis, 2022

    Insert

    CnGdmB (CNE_RS35790 Cupriavidus necator N-1)
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEUK066

    Plasmid

    #180830

    Purpose

    T7-inducible expression construct to produce NaGdmA (SARO_RS07455) in E. coli

    Depositor

    Gregg Beckham

    Article

    Bleem et al Chem Catalysis, 2022

    Insert

    NaGdmA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMC-9-35S-11

    Plasmid

    #176881

    Purpose

    MoClo Level 0 plasmid, contains 35S

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    35S terminator
    

    Use

    Tags

    Expression

    Plant

    Mutation

    WT

    Promoter

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SaCas9-mSA

    Plasmid

    #182953

    Purpose

    Express SaCas9-mSA

    Depositor

    Wen Xue

    Article

    Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

    Insert

    SaCas9-mSA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJEP206-AAV-1.3CaMKII-MCS-pA.

    Plasmid

    #78319

    Purpose

    AAV vector backbone containing a 1.3CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    1.3CaMKIIa

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJEP210-AAV-0.4CaMKII P-MCS-pA

    Plasmid

    #63084

    Purpose

    AAV2 vector backbone containing a 0.4CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    0.4αCaMKII

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJEP222-AAV-CMV-GFP-pA

    Plasmid

    #62916

    Purpose

    AAV vector backbone designed to express GFP from a CMV promoter. It also contains a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJEP220-AAV-0.5Synapsin-GFP-pA

    Plasmid

    #62920

    Purpose

    AAV vector backbone designed to express GFP from a 0.5Synapsin promoter. It also contains a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    0.5synapsin

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJEP218-AAV-1.3αCaMKII-GFP-pA

    Plasmid

    #62922

    Purpose

    AAV vector backbone designed to express GFP from a 1.3CaMKIIa promoter.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    1.3αCaMKII

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJEP219-AAV-1.1Synapsin-GFP-pA

    Plasmid

    #62921

    Purpose

    AAV vector backbone designed to express GFP from a 1.1Synapsin promoter. It also contains a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    1.1Synapsin

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRSFDuetMP-CeSmg7_1-395-G80E_S

    Plasmid

    #147476

    Purpose

    Bacterial Expression of CeSmg7_1-395-G80E

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

    Insert

    CeSmg7_1-395-G80E (smg-7 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Antares2-N1

    Plasmid

    #120869

    Purpose

    Piggybac transposon plasmid for live animal optical imaging

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    Akaluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PB-ReNL

    Plasmid

    #123138

    Purpose

    Piggybac transposon plasmid for live animal optical imaging of Red-enhanced nanolantern

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    Red-enhanced Nanolantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. BPK617

    Plasmid

    #179297

    Purpose

    Expresses dCas9 fused to VP64

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    dCas9-VP64
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    CAG

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge