We narrowed to 10,197 results for: otos

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AfrLEA3m_mCh-2xFKBP (pBS1137)

Plasmid

#185321

Purpose

For the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AfrLEA3m

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_E4_47-300 (pBS0847)

Plasmid

#185279

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E4_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E4_47-300

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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myc-hRAD18 dC2

Plasmid

#68831

Purpose

Expresses c-myc-tagged human RAD18 deleting Polymerase eta binding domain

Depositor

Satoshi Tateishi

Article

Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

Insert

c-myc tagged human RAD18 deleting Polymerase eta binding domain (RAD18 Human)

Tags

c-myc

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
MAC-ACE2

Plasmid

#158381

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

ACE2 (ACE2 Human)

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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dmBACCS2NS-IRES-dOrai-IRES-mCherry

Plasmid

#72896

Purpose

Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2NS-IRES-dOrai-IRES-mCherry

Tags

IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-sbGLuc-CheRiff-EGFP

Plasmid

#114108

Purpose

fusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Ikefuama et al Int J Mol Sci. 2022 Oct 27;23(21):12994.

Insert

Gaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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myc-hRAD18 d6BD

Plasmid

#68832

Purpose

Expresses c-myc-tagged human RAD18 deleting hRAD6 binding domain

Depositor

Satoshi Tateishi

Article

Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

Insert

c-myc tagged human RAD18 deleting hRAD6 binding domain (RAD18 Human)

Tags

c-myc

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only
pCI-neo-mApg5(K130R)

Plasmid

#22957

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Expression

Mammalian

Mutation

130 Lysine to Arginine

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
CAHS2parrc-mCh-2xFKBP (pBS1109)

Plasmid

#185304

Purpose

For the mammalian expression of the tardigrade protein CAHS2parrc attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2parrc

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Pcdh15_mini_V7-CD2

Plasmid

#199186

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

mmPcdh15_mini_V7-CD2

Use

AAV

Expression

Mammalian

Mutation

EC4, EC5, EC6, EC9, EC10 are deleted from msPCDH1…

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Pcdh15_mini_V8-CD2

Plasmid

#199187

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

mmPcdh15_mini_V8-CD2

Use

AAV

Mutation

EC5, EC6, EC8, EC9, EC10 are deleted from msPCDH1…

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-RNF8-N-terminus(1-140)

Plasmid

#64675

Purpose

expresses only N terminus of RNF8, amino acids 1 to 140

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Ring finger protein 8 (RNF8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

only N terminus amino acids 1-140

Promoter

CMV

Available Since

May 20, 2015

Availability

Academic Institutions and Nonprofits only
CMV-REX-GECO0.9

Plasmid

#61247

Purpose

Expresses REX-GECO0.9 in mammalian cells

Depositor

Robert Campbell

Article

Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

Insert

REX-GECO0.9

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1: P60R, V61W, R6…

Promoter

CMV

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only
SFG.CNa22_opt.IRES.eGFP

Plasmid

#22491

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

Codon optimised Calcineurin A mutant 22 (PPP3CA Human)

Use

Retroviral

Mutation

V314R; Y341F

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pT2EFCRET(#324)

Plasmid

#184059

Purpose

cyclofen-inducible CRE activation in zebrafish transient transgenic

Depositor

Sophie Vriz

Article

Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

Insert

CRE-ERT2

Use

Zebrafish transient transgenesis

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Xl-EF1a

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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LRG-2.1T-neo-sgTAF12(human)#4.4

Plasmid

#105590

Purpose

lentivirally express gRNA targeting human TAF12 HFD with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

gRNA targeting human TAF12 HFD

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-WRAP53beta-DC-mutant-G435R

Plasmid

#64684

Purpose

expresses single mutated WRAP53beta at G435R, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutation at G435R

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-DC-mutant-R398W

Plasmid

#64683

Purpose

expresses single mutated WRAP53beta at R398W, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutation at R398W

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
px335-sgRNA-EF(Cas9wt)-Rif1

Plasmid

#122306

Purpose

Expresses sgRNA targeting mouse Rif1 and Cas9 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Rif1 (Rif1 Synthetic)

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-puro/hFOXH1 shRNA1

Plasmid

#59303

Purpose

Knockdown of human FOXH1

Depositor

Shinya Yamanaka

Article

Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

Insert

hFOXH1 shRNA (FOXH1 Human)

Use

RNAi and Retroviral

Promoter

hU6

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only