We narrowed to 53,425 results for: plasmid

1. pLD-puro-Cc-IFIT5_WT-VA

   Plasmid

   #191221

   Purpose

   Lentiviral expression of human IFIT5_WT in mammalian cells

   Depositor

   Mohan Babu

   Insert

   interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
   

   Use

   Lentiviral

   Tags

   VA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLD-puro-Cc-SARS-CoV-2_NSP3-VA

   Plasmid

   #191214

   Purpose

   Lentiviral expression of SARS-CoV-2 NSP3 in mammalian cells

   Depositor

   Mohan Babu

   Insert

   Non structural protein 3 (ORF1ab SARS CoV-2)
   

   Use

   Lentiviral

   Tags

   VA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Rat SARE-Arc Min Promoter-Flag Arc-Arc Genomic 3'UTR

   Plasmid

   #233056

   Purpose

   To express Flag-tagged Rat Arc from a Rat SARE-Arc minimal promoter. The Arc gene contains the Rat Arc native Genomic 3'UTR containing two introns

   Depositor

   Jonathan Ploski

   Insert

   Rat Arc (Arc Rat)
   

   Use

   AAV

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJAT22

   Plasmid

   #204311

   Purpose

   For generating targeting inversions. N-terminal split GFP, expressed in eyes.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-GFPn
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   3XP3

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCJ1022

   Plasmid

   #228762

   Purpose

   Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.

   Depositor

   Bradley Yoder

   Insert

   Pkd2 gRNAs (Pkd2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SJZ6-HIS5

   Plasmid

   #228259

   Purpose

   Template plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   his5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 S.c.

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. SJZ2-TRP1

   Plasmid

   #228256

   Purpose

   Template plasmid for yeast gene knockout, uses auxotrophic marker TRP1 (Kluyveromyces lactis)

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   TRP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   BBC1 S.c.

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SJZ10-LEU2

   Plasmid

   #228262

   Purpose

   Template plasmid for yeast gene knockout, uses auxotrophic marker LEU2 (Kluyveromyces lactis)

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   LEU2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   LEU2 K.l.

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CMV-dSa VP64 Afp

   Plasmid

   #99697

   Purpose

   Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAV

   Depositor

   George Church

   Insert

   dCas9 and gRNA targeting Afp (Afp Synthetic)
   

   Use

   AAV and CRISPR

   Tags

   VP64

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS416 CUP1pro-Htt103QdeltaP125-134-YFP

    Plasmid

    #224882

    Purpose

    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 10 amino acids of the proline-rich region (125-134) tagged with YFP using copper.

    Depositor

    Paul Herman

    Insert

    Htt103QdeltaP125-134 (HTT Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    deltaP125-134

    Promoter

    CUP1

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS416 CUP1pro-Htt103QdeltaP135-170-YFP

    Plasmid

    #224878

    Purpose

    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 36 amino acids of the proline-rich region (135-170) tagged with YFP using copper.

    Depositor

    Paul Herman

    Insert

    Htt103QdeltaP135-170 (HTT Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    deltaP135-170

    Promoter

    CUP1

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS416 CUP1pro-Htt103QdeltaP141-160-YFP

    Plasmid

    #224886

    Purpose

    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the middle 20 amino acids of the proline-rich region (141-160) tagged with YFP using copper.

    Depositor

    Paul Herman

    Insert

    Htt103QdeltaP141-160 (HTT Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    deltaP141-160

    Promoter

    CUP1

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRS416 CUP1pro-Htt103QdeltaP125-160-YFP

    Plasmid

    #224883

    Purpose

    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 36 amino acids of the proline-rich region (125-160) tagged with YFP using copper.

    Depositor

    Paul Herman

    Insert

    Htt103QdeltaP125-160 (HTT Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    deltaP125-160

    Promoter

    CUP1

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS416 CUP1pro-Htt103QdeltaP141-170-YFP

    Plasmid

    #224880

    Purpose

    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 30 amino acids of the proline-rich region (141-170) tagged with YFP using copper.

    Depositor

    Paul Herman

    Insert

    Htt103QdeltaP141-170 (HTT Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    deltaP141-170

    Promoter

    CUP1

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS416 CUP1pro-Htt103QdeltaP161-170-YFP

    Plasmid

    #224876

    Purpose

    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 10 amino acids of the proline-rich region (161-170) tagged with YFP using copper.

    Depositor

    Paul Herman

    Insert

    HTT103QdeltaP161-170 (HTT Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    delta161-170

    Promoter

    CUP1

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. SOX2-3'gRNA

    Plasmid

    #215543

    Purpose

    gRNA targeting SOX2 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Insert

    SOX2 (SOX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. SOX2-P2A-NLS-mClover3-IRES-Puro

    Plasmid

    #215544

    Purpose

    Donor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus

    Depositor

    Kai Wang

    Insert

    SOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGU06

    Plasmid

    #212148

    Purpose

    Plasmid expressing alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase and tyrosine aminotransferase under constitutive promoter PgyrA control.

    Depositor

    Kang Zhou

    Insert

    alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase, tyrosine aminotransferase (AGX1, alaA, tyrB Budding Yeast, Agx1: S. cerevisiae; alaA, tyrB: E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PgyrA

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pnCas9-AID

    Plasmid

    #196242

    Purpose

    Plasmid expressing the nCas9-AID fusion

    Depositor

    Andrea Puhar

    Article

    Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

    Insert

    nickase Cas9 fused to AID
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYCTK010

    Plasmid

    #176714

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Kazachstania africana (sender part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    mfalpha1Ka
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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