We narrowed to 6,522 results for: sas

1. pLV-CMV-EGFP-PolB-PAMmut-Hygro

   Plasmid

   #176086

   Purpose

   EGFP fused to the N-terminus of a POLB containing a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Mutation in the PAM site used by PolBKOg1

   Promoter

   CMV

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE-NLS-HA-TurboID-PCNA

   Plasmid

   #215074

   Purpose

   Expresses NLS-HA-TurboID-PCNA in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   PCNA (PCNA Human)
   

   Use

   Retroviral

   Tags

   NLS-HA-TurboID

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. miniTol2 x EF1a_EKAREN5 + PGK-blast

   Plasmid

   #167818

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5
   

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-TetOne-FFSS-Cyclin D1 (T286A)

   Plasmid

   #215147

   Purpose

   Expresses FFSS-Cyclin D1 (T286A) in mammalian cells from an inducible lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   FFSS (FLAG-FLAG-STREP-STREP)

   Expression

   Mammalian

   Mutation

   With T286A mutation.

   Promoter

   TRE3GS

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-basic-FTO promoter_WT

   Plasmid

   #108922

   Purpose

   The core promoter region of FTO was inserted into pGL3 basic vector

   Depositor

   Jianjun Chen

   Article

   Su et al Cell. 2018 Jan 11;172(1-2):90-105.e23. d

   Insert

   FTO (FTO Human)
   

   Tags

   NA

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3-basic-FTO promoter_Mut

   Plasmid

   #108923

   Purpose

   The core promoter region of FTO with mutant CEBPA binding sites was inserted into pGL3 basic vector

   Depositor

   Jianjun Chen

   Article

   Su et al Cell. 2018 Jan 11;172(1-2):90-105.e23. d

   Insert

   FTO (FTO Human)
   

   Tags

   NA

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-APTX

   Plasmid

   #215128

   Purpose

   Expresses mCherry-APTX in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   APTX (APTX Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p21-mCerulean-NeoR

   Plasmid

   #215080

   Purpose

   Expresses p21-mCerulean in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDKN1A (CDKN1A Human)
   

   Tags

   mCerulean

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-CMV-PdCO-EGFP

   Plasmid

   #198512

   Purpose

   Expresses optimized PdCO in frame with EGFP under control of CMV promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Insert

   PdCO
   

   Tags

   EGFP and Rho1D4

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVRC8400-SARS-CoV-2-S2P-B.1.617.2-AVI

    Plasmid

    #176334

    Purpose

    Expresses SARS-CoV-2 S2P protein from the B.1.617.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.617.2 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19R, G142D, del156-157, R158G, L452R, T478K, D61…

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. miniTol2 x EF1a_EKAREN5(TA) + PGK-puro

    Plasmid

    #167819

    Purpose

    control sensor for EKAREN5

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5(TA)
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHL-EF1a-SphcCas9(D10A)-iP-A

    Plasmid

    #60600

    Purpose

    Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Inserts

    CRISPR Cas9 D10A
    puromycin resistance gene
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Changed Asp 10 to Ala, Codon usage optimized for …

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVRC8400-SARS-CoV-2-RBD-BA.2-AVI

    Plasmid

    #184533

    Purpose

    Expresses SARS-CoV-2 RBD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-BA.2 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G339D, S371F, S373P, S375F, T376A, D405N, R408S, …

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVRC8400-SARS-CoV-2-RBD-E484K-AVI

    Plasmid

    #176444

    Purpose

    Expresses SARS-CoV-2 RBD domain containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-E484K (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    E484K

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. miniTol2 x EF1a_EKAREN4(TA) + PGK-blast

    Plasmid

    #167827

    Purpose

    Control sensor for EKAREN4

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4(TA)
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. miniTol2 x EF1a_EKAREN5(TA) + PGK-blast

    Plasmid

    #167820

    Purpose

    control sensor for EKAREN5

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5(TA)
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVX-CMV-XRCC1-gRNA res-Neo

    Plasmid

    #176149

    Purpose

    XRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2 & a neomycin/G418 resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    X-ray repair cross complementing 1 (XRCC1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Dual PAM resistance to XRCC1 gRNA 1 and XRCC1 gRN…

    Promoter

    CMV

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVRC8400-SARS-CoV-2-RBD-SD1-L452R-T478K-AVI

    Plasmid

    #176456

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain containing L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-L452R-T478K (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    L452R, T478K

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVRC8400-SARS-CoV-2-S2P-B.1.1.7-AVI

    Plasmid

    #176325

    Purpose

    Expresses SARS-CoV-2 S2P protein from the B.1.1.7 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.1.7 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    69-70 del, 144del, N501Y, A570D, D614G, P681H, T7…

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. miniTol2 x EF1a_EKAREN4 + PGK-puro

    Plasmid

    #167824

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge